Title:
A METHOD AND KIT FOR DETECTING THE EARLY ONSET OF RENAL TUBULAR CELL INJURY
Document Type and Number:
WIPO Patent Application WO2004088276
Kind Code:
A3
Abstract:
A method and kit for detecting the early onset of renal tubular cell injury, utilizing NGAL as an early urinary biomarker. NGAL is a small secreted polypeptide that is protease resistant and consequently readily detected in the urine following renal tubule cell injury. NGAL protein expression is detected predominantly in proximal tubule cells, in a punctate cytoplasmic distribution reminiscent of a secreted protein. The appearance NGAL in the urine is related to the dose and duration of renal ischemia and nephrotoxemia, and is diagnostic of renal tubule cell injury and renal failure. NGAL detection is also a useful marker for monitoring the nephrotoxic side effects of drugs or other therapeutic agents.
Inventors:
DEVARAJAN PRASED (US)
BARASCH JONATHAN M (US)
BARASCH JONATHAN M (US)
Application Number:
PCT/US2004/009191
Publication Date:
November 25, 2004
Filing Date:
March 26, 2004
Export Citation:
Assignee:
CHILDRENS HOSP MEDICAL CENTER (US)
UNIV COLUMBIA (US)
DEVARAJAN PRASED (US)
BARASCH JONATHAN M (US)
UNIV COLUMBIA (US)
DEVARAJAN PRASED (US)
BARASCH JONATHAN M (US)
International Classes:
A61K31/675; G01N33/53; G01N; (IPC1-7): G01N33/53
Foreign References:
| US6447989B1 | 2002-09-10 |
Other References:
YAN L. ET AL: "The high molecular weight urinary matrix metalloproteinase (MMP) activity is a complex of gelatinase B/MMP-9 and neutrophil gelatinase-associated lipocalin (NGAL).", J. BIOL. CHEM., vol. 276, no. 40, 5 October 2001 (2001-10-05), pages 37258 - 37265, XP002226670
MONIER ET AL: "Gelatinase isoforms in urine from bladder cancer patients", CLINICA CHIMICA ACTA, vol. 299, no. 1-2, September 2000 (2000-09-01), pages 11 - 23, XP002229630
ELNEIHOUM ET AL: "Leukocyte activation detected by increased plasma levels of inflammatory mediators in patients with ischemic cerebrovascular diseases", STROKE, vol. 27, 1996, pages 1734 - 1738, XP002982102
MONIER ET AL: "Gelatinase isoforms in urine from bladder cancer patients", CLINICA CHIMICA ACTA, vol. 299, no. 1-2, September 2000 (2000-09-01), pages 11 - 23, XP002229630
ELNEIHOUM ET AL: "Leukocyte activation detected by increased plasma levels of inflammatory mediators in patients with ischemic cerebrovascular diseases", STROKE, vol. 27, 1996, pages 1734 - 1738, XP002982102
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