Title:
METHOD AND KIT FOR DETECTING MULTIPLE TYPES OF TARGET NUCLEIC ACID FRAGMENTS
Document Type and Number:
WIPO Patent Application WO/2023/195542
Kind Code:
A1
Abstract:
A method for detecting multiple types of target nucleic acid fragments in a sample, said method comprising: step (a) for contacting the sample with gRNAs, Cas and a substrate nucleic acid fragment, wherein gRNA forms a binary complex with Cas and is fixed to the surface of a particle, there are multiple identifiable types of particles, a specific type of gRNA is fixed to a specific type of particle, each particle is placed within a minute reaction space, the contact is performed within the reaction space, and, as a result, when a target nucleic acid fragment is present in the reaction space, a ternary complex is formed and the substrate nucleic acid fragment is cleaved to emit fluorescence; step (b) for identifying the type of particle placed in the reaction space; and step (c) for detecting the fluorescence in the reaction space. In this method, the detection of fluorescence indicates the presence of a target nucleic acid fragment in the reaction space, and the type of the particle corresponds to the type of the target nucleic acid fragment.
Inventors:
WATANABE RIKIYA (JP)
SHINODA HAJIME (JP)
MAKINO ASAMI (JP)
IIDA TATSUYA (JP)
YOSHIMURA MAMI (JP)
SHINODA HAJIME (JP)
MAKINO ASAMI (JP)
IIDA TATSUYA (JP)
YOSHIMURA MAMI (JP)
Application Number:
PCT/JP2023/014520
Publication Date:
October 12, 2023
Filing Date:
April 10, 2023
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C12Q1/34; C12M1/34; C12N9/16; C12N9/22; C12N15/09; C12Q1/68; C12Q1/6876; G01N37/00
Domestic Patent References:
| WO2021188734A1 | 2021-09-23 | |||
| WO2021002476A1 | 2021-01-07 | |||
| WO2022145354A1 | 2022-07-07 | |||
| WO2022075313A1 | 2022-04-14 |
Other References:
SHINODA HAJIME, TAGUCHI YUYA, NAKAGAWA RYOYA, MAKINO ASAMI, OKAZAKI SAE, NAKANO MASAHIRO, MURAMOTO YUKIKO, TAKAHASHI CHIHARU, TAKA: "Amplification-free RNA detection with CRISPR–Cas13", COMMUNICATIONS BIOLOGY, vol. 4, no. 1, 1 December 2021 (2021-12-01), pages 1 - 7, XP055948638, DOI: 10.1038/s42003-021-02001-8
SHINODA HAJIME, IIDA TATSUYA, MAKINO ASAMI, YOSHIMURA MAMI, ISHIKAWA JUNICHIRO, ANDO JUN, MURAI KAZUE, SUGIYAMA KATSUMI, MURAMOTO : "Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis", COMMUNICATIONS BIOLOGY, vol. 5, no. 1, XP093097195, DOI: 10.1038/s42003-022-03433-6
SHINODA HAJIME, IIDA TATSUYA, MAKINO ASAMI, YOSHIMURA MAMI, ISHIKAWA JUNICHIRO, ANDO JUN, MURAI KAZUE, SUGIYAMA KATSUMI, MURAMOTO : "Automated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosis", COMMUNICATIONS BIOLOGY, vol. 5, no. 1, XP093097195, DOI: 10.1038/s42003-022-03433-6
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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