Title:
METHOD AND KIT FOR DETECTING TRICHOPHYTON
Document Type and Number:
WIPO Patent Application WO/2017/061618
Kind Code:
A1
Abstract:
The objective of the present invention is to provide a method and a kit which are capable of easily detecting whether trichophyton is present or not in a target region of the skin of a subject. In order to achieve this objective, the present invention provides a method for detecting whether trichophyton is present or not in a target region of the skin of a subject, the method comprising the steps of: (a) preparing a first reagent, which includes a first support having a polar surface and a release substance which is derived from the target region and adhered to the polar surface, and a second reagent which includes a second support and a keratinase substrate bound to the surface of the second support; (b) after step (a), bringing the release substance in the first reagent into contact with the substrate in the second reagent under a condition in which the enzymatic reaction of keratinase proceeds; and (c) after step (b), detecting whether the substrate in the second reagent decomposes or not.
Inventors:
TAKEHARA KIMIE (JP)
MINEMATSU TAKEO (JP)
SANADA HIROMI (JP)
MINEMATSU TAKEO (JP)
SANADA HIROMI (JP)
Application Number:
PCT/JP2016/080006
Publication Date:
April 13, 2017
Filing Date:
October 07, 2016
Export Citation:
Assignee:
UNIV TOKYO (JP)
International Classes:
C12Q1/04; C12Q1/37; G01N33/50
Domestic Patent References:
| WO2006123789A1 | 2006-11-23 |
Foreign References:
| JP2003319796A | 2003-11-11 |
Other References:
WIRTH D. F. ET AL.: "Rapid identification of Leishmania species by specific hybridization of kinetoplast DNA in cutaneous lesions", PROC. NATL ACAD. SCI. USA, vol. 79, 1 November 1982 (1982-11-01), pages 6999 - 7003, XP002082053, ISSN: 1091-6490
EL-REFAI H. A. ET AL.: "Improvement of the newly isolated Bacillus pumilus FH9 keratinolytic activity", PROCESS BIOCHEMISTRY, vol. 40, 2005, pages 2325 - 2332, XP025306749, ISSN: 1359-5113
EL-REFAI H. A. ET AL.: "Improvement of the newly isolated Bacillus pumilus FH9 keratinolytic activity", PROCESS BIOCHEMISTRY, vol. 40, 2005, pages 2325 - 2332, XP025306749, ISSN: 1359-5113
Attorney, Agent or Firm:
NAGAI Hiroshi et al. (JP)
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