METHOD OF LABELING IMMUNOGLOBULIN USING QUANTUM DOTS - SHENZHEN DAAI MEDICAL TECHNOLOGY CO LTD

Title:

METHOD OF LABELING IMMUNOGLOBULIN USING QUANTUM DOTS

Document Type and Number:

WIPO Patent Application WO/2015/054939

Kind Code:

A1

Abstract:

Method of labeling immunoglobulin using quantum dots comprises steps of cleaning quantum dots, performing activation, cleaning the quantum dots a second time, cleaning immunoglobulin, performing coupling, and performing separation. By means of structural characteristics of water-soluble quantum dots, characteristics of reactions of activation of carboxyl groups with EDC and characteristics of a condensation reaction, and through optimization of a condition of each step, carboxyl groups on surfaces of quantum dots are completely activated and are in a suitable labeling environment. Through the optimization of a condition of a coupling step, all quantum dots can be bonded to the surface of immunoglobulin through a condensation reaction, so that labeling efficiency is high. By using a reaction solution obtained after steps of exclusion, chromatography, separation, purification, and coupling, immunoglobulin that has not undergone reaction can be recycled for continued labeling, thereby improving utilization of immunoglobulin and saving cost. A manufactured protein-quantum dot conjugate has high activity and simple operations and used devices, and can achieve industrial scaled-up production.

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Inventors:

ZHANG ERYING (CN)
ZHANG GUOJIAN (CN)

Application Number:

PCT/CN2013/086883

Publication Date:

April 23, 2015

Filing Date:

November 11, 2013

Export Citation:

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Assignee:

SHENZHEN DAAI MEDICAL TECHNOLOGY CO LTD (CN)

International Classes:

G01N33/533

Foreign References:

CN103487578A	2014-01-01
CN102262157A	2011-11-30
CN101907625A	2010-12-08
CN103048460A	2013-04-17
CN103063828A	2013-04-24
CN101441212A	2009-05-27
CN103149360A	2013-06-12
CN102279265A	2011-12-14
US20070249064A1	2007-10-25

Attorney, Agent or Firm:

SHENZHEN TALENT PATENT SERVICE (CN)
深圳市精英专利事务所 (CN)

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