Title:
METHOD FOR LABELLING OF CLEAVAGE OF NUCLEIC ACID SEQUENCE
Document Type and Number:
WIPO Patent Application WO/2014/025001
Kind Code:
A1
Abstract:
[Problem] Probe sets which have been used in conventional FISH methods have a problem that the location of a labelled probe labelled with an orange fluorescent dye and the location of a labelled probe labelled with a green fluorescent dye is apart to some extent from each other and therefore both orange fluorescence and green fluorescence, rather than yellow fluorescence, are observed in some relative positions or angles between a fluorescence microscope and a target nucleic acid even when the target nucleic acid is not cleaved, which may lead to the false determination that the target nucleic acid is cleaved.
[Solution] The following steps are involved: a step of bringing a hybridization solution into contact with a target nucleic acid (1), wherein the hybridization solution contains a probe set (5) that is composed of at least two labelled probes (5a, 5b) respectively labelled with different identifying factors and can hybridize with almost the full length of the sequence for the target nucleic acid (1); and a step of causing the identifying factors to develop the functions thereof to produce signals.
Inventors:
TAKEUCHI KENGO (JP)
Application Number:
PCT/JP2013/071576
Publication Date:
February 13, 2014
Filing Date:
August 08, 2013
Export Citation:
Assignee:
JAPAN FOUND CANCER (JP)
International Classes:
G01N21/78; C12Q1/68; G01N33/53; G01N33/536
Foreign References:
| JP2007535966A | 2007-12-13 | |||
| JP2004157053A | 2004-06-03 | |||
| JP2002542793A | 2002-12-17 |
Other References:
See also references of EP 2883964A4
Attorney, Agent or Firm:
Takamatsu Takayuki (JP)
Takamatsu Dutiful (JP)
Takamatsu Dutiful (JP)
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