Title:
METHOD FOR MAINTAINING AND AMPLIFYING COLON CANCER STEM CELLS AND METHOD FOR INDUCING COLON CANCER ORGANOID
Document Type and Number:
WIPO Patent Application WO/2018/225751
Kind Code:
A1
Abstract:
The present invention provides a method for maintaining and amplifying colon cancer stem cells or for inducing a colon cancer organoid. The present invention further provides a drug screening system using colon cancer stem cells maintained and amplified by said method or a colon cancer organoid induced by said method.
Inventors:
AOI TAKASHI (JP)
ISHIDA RYO (JP)
AOI MICHIYO (JP)
KAKEJI YOSHIHIRO (JP)
ISHIDA RYO (JP)
AOI MICHIYO (JP)
KAKEJI YOSHIHIRO (JP)
Application Number:
PCT/JP2018/021624
Publication Date:
December 13, 2018
Filing Date:
June 05, 2018
Export Citation:
Assignee:
UNIV KOBE NAT UNIV CORP (JP)
International Classes:
C12N5/095; C12N5/10; C12Q1/02
Other References:
PEUKER, K. ET AL.: "Cell -specific roles of calcineurin in intestinal tumor development", UNITED EUROPEAN GASTROENTEROLOGY JOURNAL, vol. 4, no. 5, October 2016 (2016-10-01), pages A17
PEUKER, K. ET AL.: "Epithelial calcineurin controls microbiota-dependent intestinal tumor development", NATURE MEDICINE, vol. 22, no. 5, May 2016 (2016-05-01), pages 506 - 515, XP055562492
WERNECK, M. B. F. ET AL.: "Cyclosporin A inhibits colon cancer cell growth independently of the Calcineurin pathway", CELL CYCLE, vol. 11, no. 21, 1 November 2012 (2012-11-01), pages 3997 - 4008, XP055562499
NIITSU, H. ET AL.: "KRAS mutation leads to decreased expression of regulator of Calcineurin 2, resulting in tumor proliferation in colorectal cancer", ONCOGENESIS, vol. 5, no. 8, August 2016 (2016-08-01), pages e253, XP055562502
DOTTO, G. ET AL.: "Calcineurin Signaling as a Negative Determinant of Keratinocyte Cancer Stem Cell Potential and Carcinogenesis", CANCER RESEARCH, vol. 71, no. 6, 15 March 2011 (2011-03-15), pages 2029 - 2033, XP055562506
ISHIDA, R. ET AL.: "The Tissue-Reconstructing Ability of Colon CSCs Is Enhanced by FK506 and Suppressed by GSK3 Inhibition", MOLECULAR CANCER RESEARCH, vol. 15, no. 10, 14 July 2017 (2017-07-14), pages 1455 - 1466, XP055562510
PEUKER, K. ET AL.: "Epithelial calcineurin controls microbiota-dependent intestinal tumor development", NATURE MEDICINE, vol. 22, no. 5, May 2016 (2016-05-01), pages 506 - 515, XP055562492
WERNECK, M. B. F. ET AL.: "Cyclosporin A inhibits colon cancer cell growth independently of the Calcineurin pathway", CELL CYCLE, vol. 11, no. 21, 1 November 2012 (2012-11-01), pages 3997 - 4008, XP055562499
NIITSU, H. ET AL.: "KRAS mutation leads to decreased expression of regulator of Calcineurin 2, resulting in tumor proliferation in colorectal cancer", ONCOGENESIS, vol. 5, no. 8, August 2016 (2016-08-01), pages e253, XP055562502
DOTTO, G. ET AL.: "Calcineurin Signaling as a Negative Determinant of Keratinocyte Cancer Stem Cell Potential and Carcinogenesis", CANCER RESEARCH, vol. 71, no. 6, 15 March 2011 (2011-03-15), pages 2029 - 2033, XP055562506
ISHIDA, R. ET AL.: "The Tissue-Reconstructing Ability of Colon CSCs Is Enhanced by FK506 and Suppressed by GSK3 Inhibition", MOLECULAR CANCER RESEARCH, vol. 15, no. 10, 14 July 2017 (2017-07-14), pages 1455 - 1466, XP055562510
Attorney, Agent or Firm:
TAKASHIMA, Hajime et al. (JP)
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