Title:
METHOD FOR MAKING PENTOSES AND PENTOSE-BASED SOLUBLE OLIGO/POLYSACCHARIDES FROM CEREALS
Document Type and Number:
WIPO Patent Application WO/2010/088744
Kind Code:
A3
Abstract:
The present invention provides a method for the extraction and isolation of solubilised arabinoxylan depolymerisation products, using arabinoxylan-containing plant material as a starting material. Preferably, such solubilised arabinoxylan depolymerisation product is any one of soluble arabinoxylan, arabinoxylan-oligosaccharides, xylose and arabinose. Said method comprises the mashing of said arabinoxylan-containing plant material in water and treating it with an appropriate concentration of an endoxylanase enzyme preparation in order to enzymatically depolymerise and solubilise a fraction of the arabinoxylans comprised in said plant material, wherein said endoxylanase treatment is followed or preceded by an acid treatment. This acid treatment comprising the incubation of said plant material in an acidified aqueous mash in order to obtain a depolymerisation and solubilisation of another fraction of the arabinoxylans comprised in said plant material. Preferably, said acidified mash is obtained by adding to said mash a strong acid to a final concentration between 0.02 and 0.4 N. The method further comprises the separation from said mashed plant material of a water-solubilised fraction, which comprises at least part of the solubilised arabinoxylan depolymerisation products.
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Inventors:
BROEKAERT WILLEM (BE)
Application Number:
PCT/BE2010/000008
Publication Date:
November 25, 2010
Filing Date:
February 09, 2010
Export Citation:
Assignee:
FUGEIA NV (BE)
BROEKAERT WILLEM (BE)
BROEKAERT WILLEM (BE)
International Classes:
C08B37/14; C13K13/00
Foreign References:
| JP2009013088A | 2009-01-22 |
Other References:
SANJUST E ET AL: "Note. Xylose production from durum wheat bran: Enzymic versus chemical methods.", FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL, vol. 10, no. 1, February 2004 (2004-02-01), pages 11 - 14, XP009138575, ISSN: 1082-0132
DATABASE WPI Week 197924, Derwent World Patents Index; AN 1979-45182B, XP002600494
AACHARY A A ET AL: "Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB LNKD- DOI:10.1016/J.BIORTECH.2008.06.050, vol. 100, no. 2, 1 January 2009 (2009-01-01), pages 991 - 995, XP025535095, ISSN: 0960-8524, [retrieved on 20080813]
SWENNEN KATRIEN ET AL: "Large-scale production and characterisation of wheat bran arabinoxylooligosaccharides", JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, WILEY & SONS, CHICHESTER, GB LNKD- DOI:10.1002/JSFA.2470, vol. 86, no. 11, 1 August 2006 (2006-08-01), pages 1722 - 1731, XP002457409, ISSN: 0022-5142
MAES C ET AL: "Relative activity of two endoxylanases towards water-unextractable arabinoxylans in wheat bran", JOURNAL OF CEREAL SCIENCE, ACADEMIC PRESS LTD, XX LNKD- DOI:10.1016/J.JCS.2003.08.001, vol. 39, no. 2, 1 March 2004 (2004-03-01), pages 181 - 186, XP002457408, ISSN: 0733-5210
DATABASE WPI Week 197924, Derwent World Patents Index; AN 1979-45182B, XP002600494
AACHARY A A ET AL: "Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB LNKD- DOI:10.1016/J.BIORTECH.2008.06.050, vol. 100, no. 2, 1 January 2009 (2009-01-01), pages 991 - 995, XP025535095, ISSN: 0960-8524, [retrieved on 20080813]
SWENNEN KATRIEN ET AL: "Large-scale production and characterisation of wheat bran arabinoxylooligosaccharides", JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, WILEY & SONS, CHICHESTER, GB LNKD- DOI:10.1002/JSFA.2470, vol. 86, no. 11, 1 August 2006 (2006-08-01), pages 1722 - 1731, XP002457409, ISSN: 0022-5142
MAES C ET AL: "Relative activity of two endoxylanases towards water-unextractable arabinoxylans in wheat bran", JOURNAL OF CEREAL SCIENCE, ACADEMIC PRESS LTD, XX LNKD- DOI:10.1016/J.JCS.2003.08.001, vol. 39, no. 2, 1 March 2004 (2004-03-01), pages 181 - 186, XP002457408, ISSN: 0733-5210
Attorney, Agent or Firm:
LESCROART, Olivier (Heverlee, BE)
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