Title:
METHOD FOR MANUFACTURING ANTIBODY-DRUG CONJUGATE AND ENZYME USED FOR SAME
Document Type and Number:
WIPO Patent Application WO/2022/211075
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a simpler ADC manufacturing method. The present invention provides a method for manufacturing an antibody-drug conjugate comprising a step for conjugating a sugar-chain homogenization antibody and a sugar-chain-bound drug, the sugar-chain-bound drug being one in which a drug is bound, via a linker, to a sugar chain in which a reducing terminal is oxazolinized. The sugar-chain homogenization antibody and the sugar-chain-bound drug can be handled using an endo-β-N-acetyl glucosaminidase mutant enzyme.
Inventors:
HIBINO KAZUHIRO (JP)
NAGAHARA TAKASHI (JP)
KANAMORI SATOSHI (JP)
BABA YUTARO (JP)
NARUMI HIDEKI (JP)
OHARA KEIICHIRO (JP)
NISHIMURA YUTAKA (JP)
KAWASAKI MAYU (JP)
NAKANO SHOGO (JP)
ITO SOHEI (JP)
NAGAHARA TAKASHI (JP)
KANAMORI SATOSHI (JP)
BABA YUTARO (JP)
NARUMI HIDEKI (JP)
OHARA KEIICHIRO (JP)
NISHIMURA YUTAKA (JP)
KAWASAKI MAYU (JP)
NAKANO SHOGO (JP)
ITO SOHEI (JP)
Application Number:
PCT/JP2022/016786
Publication Date:
October 06, 2022
Filing Date:
March 31, 2022
Export Citation:
Assignee:
MICROBIOPHARM JAPAN CO LTD (JP)
International Classes:
A61K47/68; A61K39/395; A61P35/00; C12N9/24; C12N15/56; C12P19/44; C07K16/30
Domestic Patent References:
| WO2017010559A1 | 2017-01-19 | |||
| WO2020196475A1 | 2020-10-01 | |||
| WO2013120066A1 | 2013-08-15 | |||
| WO2017124084A1 | 2017-07-20 | |||
| WO2004010957A2 | 2004-02-05 | |||
| WO2014145090A1 | 2014-09-18 |
Foreign References:
| JP2019206491A | 2019-12-05 | |||
| CN107778372A | 2018-03-09 | |||
| JP2021145548A | 2021-09-27 | |||
| US20120226024A1 | 2012-09-06 | |||
| US5821337A | 1998-10-13 |
Other References:
GIDDENS JOHN P., LOMINO JOSEPH V., AMIN MOHAMMED N., WANG LAI-XI: "Endo-F3 Glycosynthase Mutants Enable Chemoenzymatic Synthesis of Core-fucosylated Triantennary Complex Type Glycopeptides and Glycoproteins", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, US, vol. 291, no. 17, 10 March 2016 (2016-03-10), US , pages 9356 - 9370, XP055907121, ISSN: 0021-9258, DOI: 10.1074/jbc.M116.721597
DATABASE UNIPROTKB 1 June 1994 (1994-06-01), ANONYMOUS : "P36913 · EBA3_ELIME", XP055973264, retrieved from UNIPROT Database accession no. P36913
DATABASE UNIPROTKB 7 June 2017 (2017-06-07), ANONYMOUS : "A0A1V3U698 · A0A1V3U698_ELIME", XP055973262, retrieved from UNIPROT Database accession no. A0A1V3U698
MIZUNO, MAMORU ET AL.: "3B-06 Synthesis of antibody-drug conjugate (ADC) precursor using PEGylated disaccharide oxazoline derivative", 40TH ANNUAL MEETING OF JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH; OCTOBER 27-29, 2021, JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH, JP, vol. 40, 15 October 2021 (2021-10-15) - 29 October 2021 (2021-10-29), JP, pages 114, XP009540124
NAT. BIOTECHNOL., vol. 23, 2005, pages 1137 - 1146
CANCER SCI., vol. 100, 2009, pages 1566 - 1572
CLIN. CANCER RES., vol. 7, 2001, pages 1490 - 1496
MABS, vol. 4, 2012, pages 458 - 465
NAT. BIOTECHNOL., vol. 26, 2008, pages 952 - 932
CHEM. SOC. REV., vol. 46, 2017, pages 5128 - 5146
J. AM. CHEM. SOC., vol. 134, 2012, pages 12308 - 12318
J. BIOL. CHEM., vol. 291, 2016, pages 16508 - 16518
NAT. PROTOC., vol. 6, 2011, pages 1183 - 1191
BIOCONJUG. CHEM., vol. 30, 2019, pages 1343 - 1355
CHEM, REV., vol. 113, 2013, pages 4905 - 4979
METHOD MOL. BIOL., vol. 2078, 2020, pages 83 - 97
CHEM. SOC. REV., vol. 48, 2019, pages 4361 - 4374
PROTEIN CELL, vol. 9, no. 1, 2018, pages 33 - 46
BIOORG. MED. CHEM., vol. 20, 2012, pages 571 - 582
J. CONTROL. RELEASE, vol. 99, 2004, pages 423 - 434
BIOCONJUGATE CHEM., vol. 28, 2017, pages 1906 - 1915
ALTSCHU ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
DATABASE UNIPROTKB 1 June 1994 (1994-06-01), ANONYMOUS : "P36913 · EBA3_ELIME", XP055973264, retrieved from UNIPROT Database accession no. P36913
DATABASE UNIPROTKB 7 June 2017 (2017-06-07), ANONYMOUS : "A0A1V3U698 · A0A1V3U698_ELIME", XP055973262, retrieved from UNIPROT Database accession no. A0A1V3U698
MIZUNO, MAMORU ET AL.: "3B-06 Synthesis of antibody-drug conjugate (ADC) precursor using PEGylated disaccharide oxazoline derivative", 40TH ANNUAL MEETING OF JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH; OCTOBER 27-29, 2021, JAPANESE SOCIETY OF CARBOHYDRATE RESEARCH, JP, vol. 40, 15 October 2021 (2021-10-15) - 29 October 2021 (2021-10-29), JP, pages 114, XP009540124
NAT. BIOTECHNOL., vol. 23, 2005, pages 1137 - 1146
CANCER SCI., vol. 100, 2009, pages 1566 - 1572
CLIN. CANCER RES., vol. 7, 2001, pages 1490 - 1496
MABS, vol. 4, 2012, pages 458 - 465
NAT. BIOTECHNOL., vol. 26, 2008, pages 952 - 932
CHEM. SOC. REV., vol. 46, 2017, pages 5128 - 5146
J. AM. CHEM. SOC., vol. 134, 2012, pages 12308 - 12318
J. BIOL. CHEM., vol. 291, 2016, pages 16508 - 16518
NAT. PROTOC., vol. 6, 2011, pages 1183 - 1191
BIOCONJUG. CHEM., vol. 30, 2019, pages 1343 - 1355
CHEM, REV., vol. 113, 2013, pages 4905 - 4979
METHOD MOL. BIOL., vol. 2078, 2020, pages 83 - 97
CHEM. SOC. REV., vol. 48, 2019, pages 4361 - 4374
PROTEIN CELL, vol. 9, no. 1, 2018, pages 33 - 46
BIOORG. MED. CHEM., vol. 20, 2012, pages 571 - 582
J. CONTROL. RELEASE, vol. 99, 2004, pages 423 - 434
BIOCONJUGATE CHEM., vol. 28, 2017, pages 1906 - 1915
ALTSCHU ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
Attorney, Agent or Firm:
SIKS & CO. (JP)
Download PDF: