Title:
METHOD FOR MANUFACTURING CELL AGGREGATES OF PLURIPOTENT STEM CELLS AND CELL AGGREGATE MANUFACTURING SYSTEM
Document Type and Number:
WIPO Patent Application WO/2015/159950
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for manufacturing cell aggregates of pluripotent stem cells. In order to solve said problem, a method for manufacturing cell aggregates of pluripotent stem cells is provided, the method comprising: (A) the thawing of pluripotent stem cells that have been separated into single cells and frozen; and (B) the formation of the thawed pluripotent stem cells into cell aggregates.
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Inventors:
GOMI SHINICHI (JP)
HARA KEISUKE (GB)
NISHISHITA NAOKI (JP)
MURAMATSU MARIE (JP)
HARA KEISUKE (GB)
NISHISHITA NAOKI (JP)
MURAMATSU MARIE (JP)
Application Number:
PCT/JP2015/061716
Publication Date:
October 22, 2015
Filing Date:
April 16, 2015
Export Citation:
Assignee:
TOKYO ELECTRON LTD (JP)
International Classes:
C12N5/0735; C12M1/00; C12N1/04; C12N5/10
Domestic Patent References:
| WO2010103748A1 | 2010-09-16 | |||
| WO2014115799A1 | 2014-07-31 | |||
| WO2013187077A1 | 2013-12-19 |
Foreign References:
| JP2013240308A | 2013-12-05 |
Other References:
IMAIZUMI K. ET AL.: "A Simple and Highly Effective Method for Slow-Freezing Human Pluripotent Stem Cells Using Dimethyl Sulfoxide, Hydroxyethyl Starch and Ethylene Glycol", PLOS ONE, vol. 9, no. Issue 2, February 2014 (2014-02-01), pages 1 - 11, XP055231301
MIYAZAKI T. ET AL.: "Optimization of Slow Cooling Cryopreservation for Human Pluripotent Stem Cells", GENESIS, vol. 52, no. 1, January 2014 (2014-01-01), pages 49 - 55, XP055231316, [retrieved on 20131119]
LI Y. ET AL.: "Bioprocessing of Cryopreservation for Large-Scale Banking of Human Pluripotent Stem Cells", BIORESEARCH OPEN ACCESS, vol. 1, no. 5, October 2012 (2012-10-01), pages 205 - 214, XP055231320
KANJI YAHIRO ET AL.: "Introduction of stem cell culture, differentiation and assay tools", THE CELL, vol. 44, no. 9, 2012, pages 393 - 397
"Hito Kansaibo Sangyo Oyo Sokushin Kiban Gijutsu Kaihatsu/Hito Kansaibo Jitsuyoka ni Muketa Hyoka Kiban Gijutsu no Kaihatsu, 'Hito Kansaibo Jitsuyoka ni Muketa Hyoka Kiban Gijutsu no Kaihatsu' Dai 1 Kai Bunkakai (Chukan Hyoka) Shiryo 5-2, New Energy and Industrial Technology Development Organization", IRYO GIJUTSUBU, 26 June 2013 (2013-06-26), pages III-2-18 - III-2-25, Retrieved from the Internet
NISHISHITA N. ET AL.: "An effective freezing/ thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions", AM J STEM CELLS., vol. 4, no. 1, March 2015 (2015-03-01), pages 38 - 49
STOVER, A.E. ET AL.: "The Generation of Embryoid Bodies from Feeder-Based or Feeder- Free Human Pluripotent Stem Cell Cultu", METHODS IN MOLECULAR BIOLOGY, vol. 767, 2011, pages 391 - 398
MIYAZAKI T. ET AL.: "Optimization of Slow Cooling Cryopreservation for Human Pluripotent Stem Cells", GENESIS, vol. 52, no. 1, January 2014 (2014-01-01), pages 49 - 55, XP055231316, [retrieved on 20131119]
LI Y. ET AL.: "Bioprocessing of Cryopreservation for Large-Scale Banking of Human Pluripotent Stem Cells", BIORESEARCH OPEN ACCESS, vol. 1, no. 5, October 2012 (2012-10-01), pages 205 - 214, XP055231320
KANJI YAHIRO ET AL.: "Introduction of stem cell culture, differentiation and assay tools", THE CELL, vol. 44, no. 9, 2012, pages 393 - 397
"Hito Kansaibo Sangyo Oyo Sokushin Kiban Gijutsu Kaihatsu/Hito Kansaibo Jitsuyoka ni Muketa Hyoka Kiban Gijutsu no Kaihatsu, 'Hito Kansaibo Jitsuyoka ni Muketa Hyoka Kiban Gijutsu no Kaihatsu' Dai 1 Kai Bunkakai (Chukan Hyoka) Shiryo 5-2, New Energy and Industrial Technology Development Organization", IRYO GIJUTSUBU, 26 June 2013 (2013-06-26), pages III-2-18 - III-2-25, Retrieved from the Internet
NISHISHITA N. ET AL.: "An effective freezing/ thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions", AM J STEM CELLS., vol. 4, no. 1, March 2015 (2015-03-01), pages 38 - 49
STOVER, A.E. ET AL.: "The Generation of Embryoid Bodies from Feeder-Based or Feeder- Free Human Pluripotent Stem Cell Cultu", METHODS IN MOLECULAR BIOLOGY, vol. 767, 2011, pages 391 - 398
Attorney, Agent or Firm:
KATSUNUMA Hirohito et al. (JP)
Katsunuma Hirohito (JP)
Katsunuma Hirohito (JP)
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