Title:
METHOD FOR MANUFACTURING CILIARY MARGIN STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2015/107738
Kind Code:
A1
Abstract:
The present invention provides a method for manufacturing ciliary margin stem cells differentiated from pluripotent stem cells, the method comprising step (1) and/or step (2) below:
(1) a step for obtaining retinospheres by suspension culturing of cells obtained from cell aggregates comprising ciliary margin-like structures differentiated from pluripotent stem cells; and
(2) a step for isolating stage specific embryonic antigen-1-positive cells from cells obtained from cell aggregates comprising ciliary margin-like structures differentiated from pluripotent stem cells.
The present invention makes it possible to manufacture efficiently and with high purity ciliary margin stem cells with the ability to differentiate to retinal cells comprising retinal layer-specific neurons and the ability to self-replicate.
Inventors:
KUWAHARA ATSUSHI (JP)
SASAI YOSHIKI 笹井 芳
SASAI YOSHIKI 笹井 芳
Application Number:
PCT/JP2014/077603
Publication Date:
July 23, 2015
Filing Date:
October 16, 2014
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C12N5/071; A61K35/44; A61L27/00; A61P27/02; A61P43/00; C12N5/074; C12N5/10; C12Q1/02
Domestic Patent References:
| WO2013183774A1 | 2013-12-12 | |||
| WO2012173207A1 | 2012-12-20 | |||
| WO2013183774A1 | 2013-12-12 | |||
| WO1998030679A1 | 1998-07-16 |
Foreign References:
| JP2012245007A | 2012-12-13 | |||
| JP2013128477A | 2013-07-04 | |||
| JP2013128476A | 2013-07-04 |
Other References:
CENTANIN L. ET AL.: "Fate Restriction and Multipotency in Retinal Stem Cells.", CELL STEM CELL, vol. 9, 2011, pages 553 - 562, XP028335474
YAKOUBI W.E. ET AL.: "Hes4 Controls Proliferative Properties of Neural Stem Cells During Retinal Ontogenesis.", STEM CELLS, vol. 30, 2012, pages 2784 - 2795, XP055213168
KOSO H. ET AL.: "CD 138/syndecan-1 and SSEA-1 mark distinct populations of developing ciliary epithelium that are regulated differentially by Wnt signal.", STEM CELLS, vol. 26, no. 12, 11 December 2008 (2008-12-11), pages 3162 - 3171, XP055213170
KOSO H. ET AL.: "SSEA-1 marks regionally restricted immature subpopulations of embryonic retinal progenitor cells that are regulated by the Wnt signaling pathway.", DEVELOPMENTAL BIOLOGY, vol. 292, no. 1, 2006, AMSTERDAM,NL, pages 265 - 276, XP024943911
BAHRAMI S.B. ET AL.: "Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells.", METHODS IN MOLECULAR BIOLOGY, vol. 916, 2012, pages 81 - 96, XP055213174
HUANG X. ET AL.: "Isolation and Functional Characterization of Pluripotent Stem Cell Derived Cardiac Progenitor Cells.", CURRENT PROTOCOLS IN STEM CELL BIOLOGY, vol. 14, 2010, HOBOKEN, NJ, USA, pages 1F.10.1 - 14, XP055213178
PANCHISION D.M ET AL.: "Optimized Flow Cytometric Analysis of Central Nervous System Tissue Reveals Novel Functional Relationships Among Cells Expressing CD 133, CD 15, and CD 24", STEM CELLS, vol. 25, 2007, pages 1560 - 1570, XP002496165
DEVELOPMENTAL DYNAMICS, vol. 239, 2010, pages 2066 - 2077
PROC. NATL. ACAD. SCI. USA, vol. 101, 2004, pages 15772 - 15777
DEVELOPMENT, vol. 136, 2009, pages 1823 - 1833
CELL, vol. 131, no. 5, 2007, pages 861 - 872
CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI K; YAMANAKA S., CELL, vol. 126, no. 4, 2006, pages 663 - 676
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
"Manipulating the Mouse Embryo, A Laboratory Manual Second Edition,", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Gene Targeting, A Practical Approach", 1993, IRL PRESS AT OXFORD UNIVERSITY PRESS
"Biomanual Series 8", 1995, YODOSHA CO., LTD., article "Gene Targeting, Making of Mutant Mouse using ES cell"
"Molecular Cloning, A Laboratory Manual, Second Edition,", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
"Biomanual Series 8, Gene Targeting, Making of Mutant Mouse using ES cell", 1995, YODOSHA CO., LTD.
NAKANO, T. ET AL., CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 785
PROC. NATL. ACAD. SCI. USA, vol. 103, no. 25, 2006, pages 9554 - 9559
NAT. BIOTECH., vol. 25, 2007, pages 681 - 686
YAKOUBI W.E. ET AL.: "Hes4 Controls Proliferative Properties of Neural Stem Cells During Retinal Ontogenesis.", STEM CELLS, vol. 30, 2012, pages 2784 - 2795, XP055213168
KOSO H. ET AL.: "CD 138/syndecan-1 and SSEA-1 mark distinct populations of developing ciliary epithelium that are regulated differentially by Wnt signal.", STEM CELLS, vol. 26, no. 12, 11 December 2008 (2008-12-11), pages 3162 - 3171, XP055213170
KOSO H. ET AL.: "SSEA-1 marks regionally restricted immature subpopulations of embryonic retinal progenitor cells that are regulated by the Wnt signaling pathway.", DEVELOPMENTAL BIOLOGY, vol. 292, no. 1, 2006, AMSTERDAM,NL, pages 265 - 276, XP024943911
BAHRAMI S.B. ET AL.: "Isolation and Expansion of Endothelial Progenitor Cells Derived from Mouse Embryonic Stem Cells.", METHODS IN MOLECULAR BIOLOGY, vol. 916, 2012, pages 81 - 96, XP055213174
HUANG X. ET AL.: "Isolation and Functional Characterization of Pluripotent Stem Cell Derived Cardiac Progenitor Cells.", CURRENT PROTOCOLS IN STEM CELL BIOLOGY, vol. 14, 2010, HOBOKEN, NJ, USA, pages 1F.10.1 - 14, XP055213178
PANCHISION D.M ET AL.: "Optimized Flow Cytometric Analysis of Central Nervous System Tissue Reveals Novel Functional Relationships Among Cells Expressing CD 133, CD 15, and CD 24", STEM CELLS, vol. 25, 2007, pages 1560 - 1570, XP002496165
DEVELOPMENTAL DYNAMICS, vol. 239, 2010, pages 2066 - 2077
PROC. NATL. ACAD. SCI. USA, vol. 101, 2004, pages 15772 - 15777
DEVELOPMENT, vol. 136, 2009, pages 1823 - 1833
CELL, vol. 131, no. 5, 2007, pages 861 - 872
CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI K; YAMANAKA S., CELL, vol. 126, no. 4, 2006, pages 663 - 676
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
"Manipulating the Mouse Embryo, A Laboratory Manual Second Edition,", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Gene Targeting, A Practical Approach", 1993, IRL PRESS AT OXFORD UNIVERSITY PRESS
"Biomanual Series 8", 1995, YODOSHA CO., LTD., article "Gene Targeting, Making of Mutant Mouse using ES cell"
"Molecular Cloning, A Laboratory Manual, Second Edition,", 1989, COLD SPRING HARBOR LABORATORY PRESS
"Current Protocols in Molecular Biology", 1987, JOHN WILEY & SONS
"Biomanual Series 8, Gene Targeting, Making of Mutant Mouse using ES cell", 1995, YODOSHA CO., LTD.
NAKANO, T. ET AL., CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 785
PROC. NATL. ACAD. SCI. USA, vol. 103, no. 25, 2006, pages 9554 - 9559
NAT. BIOTECH., vol. 25, 2007, pages 681 - 686
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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