Title:
METHOD FOR MANUFACTURING CILIARY MARGINAL ZONE-LIKE STRUCTURE
Document Type and Number:
WIPO Patent Application WO/2015/087614
Kind Code:
A1
Abstract:
The present invention provides a method for manufacturing a cell aggregate containing a ciliary marginal zone-like structure, the method comprising a step for culturing a retinal tissue-containing cell aggregate, wherein the percentage of Chx10-positive cells present in the retinal tissue is 20% to 100%, in a serum-free medium or serum medium containing a Wnt signal pathway-affecting substance and a FGF signal pathway-inhibiting substance only in the period until cells expressing RPE65 gene appear, and then the obtained "cell aggregates in which RPE65 gene-expressing cells have not appeared" are cultured in a serum-free medium or serum medium that does not contain a Wnt signal pathway-affecting substance. The manufacturing method according to the present invention makes it possible to manufacture ciliary marginal zone-like structures with high efficiency.
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Inventors:
KUWAHARA ATSUSHI (JP)
SASAI YOSHIKI 笹井 芳
SASAI YOSHIKI 笹井 芳
Application Number:
PCT/JP2014/077602
Publication Date:
June 18, 2015
Filing Date:
October 16, 2014
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
RIKEN (JP)
RIKEN (JP)
International Classes:
C12N5/071; A61K35/44; A61L27/00; A61P27/02; A61P43/00
Domestic Patent References:
| WO2013183774A1 | 2013-12-12 | |||
| WO1998030679A1 | 1998-07-16 |
Other References:
F.KUBO ET AL.: "HAIRY1 ACTS AS A NODE DOWNSTREAM OF WNT SIGNALING TO MAINTAIN", DEVELOPMENT, vol. 136, RE, 2009, pages 1823 - 1833, XP055344393, DOI: TINAL STEM CELL-LIKE PROGENITOR CELLS IN THE CHICK
W.Z.STEPHENS ET AL.: "LOSS OF ADENOMATOUS POLYPOSIS COLI (APC) RESULTS IN AN", DEVELOPMENTAL DYNAMICS, vol. 239, no. 7, EX, 2010, pages 2066 - 2077, XP055344395, DOI: PANDED CILIARY MARGINAL ZONE IN THE ZEBRAFISH EYE
B.BHATIA ET AL.: "DISTRIBUTION OF MüLLER STEM CELLS WITHIN THE NEURAL RETINA:", EXPERIMENTAL EYE RESEARCH, vol. 89, EV, 2009, pages 373 - 382, XP026396015, DOI: IDENCE FOR THE EXISTENCE OF A CILIARY MARGIN-LIKE
T.NAKANO ET AL.: "SELF-FORMATION OF OPTIC CUPS AND STORABLE STRATIFIED NEURAL", CELL STEM CELL, vol. 10, no. 6, RE, 2012, pages 771 - 785, XP028492663, DOI: TINA FROM HUMAN ESCS
S.FUHRMANN: "WNT SIGNALING IN EYE ORGANOGENESIS", ORGANOGENESIS, vol. 42, no. 4, 2008, pages 60 - 67, XP055181039
T.INOUE ET AL., STEM CELLS, vol. 24, no. 1, 2006, pages 95 - 104, XP008155751
DEVELOPMENTAL DYNAMICS, vol. 239, 2010, pages 2066 - 2077
DEVELOPMENT, vol. 136, 2009, pages 1823 - 1833
CELL, vol. 131, no. 5, 2007, pages 861 - 872
CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI K; YAMANAKA S., CELL, vol. 126, no. 4, 2006, pages 663 - 676
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
NAKANO, T ET AL., CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 785
UENO, M ET AL., PNAS, vol. 103, no. 25, 2006, pages 9554 - 9559
WATANABE, K ET AL., NAT BIOTECH, vol. 25, 2007, pages 681 - 686
UENO, M. ET AL., PNAS, vol. 103, no. 25, 2006, pages 9554 - 9559
See also references of EP 3081637A4
W.Z.STEPHENS ET AL.: "LOSS OF ADENOMATOUS POLYPOSIS COLI (APC) RESULTS IN AN", DEVELOPMENTAL DYNAMICS, vol. 239, no. 7, EX, 2010, pages 2066 - 2077, XP055344395, DOI: PANDED CILIARY MARGINAL ZONE IN THE ZEBRAFISH EYE
B.BHATIA ET AL.: "DISTRIBUTION OF MüLLER STEM CELLS WITHIN THE NEURAL RETINA:", EXPERIMENTAL EYE RESEARCH, vol. 89, EV, 2009, pages 373 - 382, XP026396015, DOI: IDENCE FOR THE EXISTENCE OF A CILIARY MARGIN-LIKE
T.NAKANO ET AL.: "SELF-FORMATION OF OPTIC CUPS AND STORABLE STRATIFIED NEURAL", CELL STEM CELL, vol. 10, no. 6, RE, 2012, pages 771 - 785, XP028492663, DOI: TINA FROM HUMAN ESCS
S.FUHRMANN: "WNT SIGNALING IN EYE ORGANOGENESIS", ORGANOGENESIS, vol. 42, no. 4, 2008, pages 60 - 67, XP055181039
T.INOUE ET AL., STEM CELLS, vol. 24, no. 1, 2006, pages 95 - 104, XP008155751
DEVELOPMENTAL DYNAMICS, vol. 239, 2010, pages 2066 - 2077
DEVELOPMENT, vol. 136, 2009, pages 1823 - 1833
CELL, vol. 131, no. 5, 2007, pages 861 - 872
CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI K; YAMANAKA S., CELL, vol. 126, no. 4, 2006, pages 663 - 676
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
NAKANO, T ET AL., CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 785
UENO, M ET AL., PNAS, vol. 103, no. 25, 2006, pages 9554 - 9559
WATANABE, K ET AL., NAT BIOTECH, vol. 25, 2007, pages 681 - 686
UENO, M. ET AL., PNAS, vol. 103, no. 25, 2006, pages 9554 - 9559
See also references of EP 3081637A4
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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