Title:
METHOD FOR MANUFACTURING HUMAN EPIDERMAL CELL GROWTH FACTOR
Document Type and Number:
WIPO Patent Application WO/2014/097733
Kind Code:
A1
Abstract:
Disclosed is a novel means making possible the manufacture of an epidermal growth factor (EGF) with good efficiency and lower cost than conventional techniques. As a result of thoroughgoing research aimed at developing a technique for producing human EGF using plants, the inventors of the present application succeeded in finding a modified EGF coding sequence and a signal peptide coding sequence that allow production of EGF with high efficiency using a transient expression system. Using these sequences allows EGF to be produced with good efficiency even when the transient expression system of a viral vector is used.
Inventors:
SATO SEIYA (JP)
OKAWA SHOUICHIROU (JP)
YOSHINAKA KYOUJI (JP)
OKAWA SHOUICHIROU (JP)
YOSHINAKA KYOUJI (JP)
Application Number:
PCT/JP2013/078663
Publication Date:
June 26, 2014
Filing Date:
October 23, 2013
Export Citation:
Assignee:
UNIBIO CORP (JP)
International Classes:
C12N15/09; C12P21/02
Domestic Patent References:
| WO2006015057A2 | 2006-02-09 | |||
| WO2006042327A2 | 2006-04-20 |
Foreign References:
| US20030228612A1 | 2003-12-11 | |||
| US6080560A | 2000-06-27 | |||
| JP2002542828A | 2002-12-17 |
Other References:
BAI, JIE-YING ET AL.: "Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants", BIOTECHNOL. LETT., vol. 29, 2007, pages 2007 - 2012
WIRTH, SONIA ET AL.: "Expression of active human epidermal growth factor (hEGF) in tobacco plants by integrative and non-integrative systems", MOL. BREED., vol. 13, 2004, pages 23 - 35
MURRAY, ELIZABETH ET AL.: "Codon usage in plant genes", NUCLEIC ACIDS RES., vol. 17, no. 2, 1989, pages 477 - 498
WIRTH, SONIA ET AL.: "Expression of active human epidermal growth factor (hEGF) in tobacco plants by integrative and non-integrative systems", MOL. BREED., vol. 13, 2004, pages 23 - 35
MURRAY, ELIZABETH ET AL.: "Codon usage in plant genes", NUCLEIC ACIDS RES., vol. 17, no. 2, 1989, pages 477 - 498
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
Patent business corporation mountain stream international patent firm (JP)
Patent business corporation mountain stream international patent firm (JP)
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