Title:
METHOD FOR MASS PROLIFERATION OF ACTIVATED NATURAL KILLER CELLS, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/191559
Kind Code:
A1
Abstract:
The present invention relates to a method for mass proliferation of activated natural killer cells, and a use thereof. More specifically, provided is a method for inducing, differentiating, and mass-proliferating activated natural killer cells with high efficiency, by using a combination of irradiated feeder cells and specific cytokines in mononuclear cells isolated from cord blood (cord blood mononuclear cells, CBMC). According to the present invention, the method of the present invention stably provides activated-NK cells proliferated in large quantities with high purity and high cytotoxicity (increased anti-tumor activity), and thus can be useful in the treatment of diseases such as anticancer treatment.
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Inventors:
KIM JI YOUNG (KR)
LEE SU JEONG (KR)
KANG HYUN JUNG (KR)
MUN JI YEONG (KR)
LEE SU JEONG (KR)
KANG HYUN JUNG (KR)
MUN JI YEONG (KR)
Application Number:
PCT/KR2023/004314
Publication Date:
October 05, 2023
Filing Date:
March 30, 2023
Export Citation:
Assignee:
CURE THERAPEUTICS INC (KR)
International Classes:
C12N5/0783; C12N13/00
Foreign References:
| CN111172115A | 2020-05-19 | |||
| CN113403273A | 2021-09-17 |
Other References:
HARADA HIDEKI, WATANABE SATORU, SAIJO KAORU, ISHIWATA ISAMU, OHNO TADAO: "A Wilms tumor cell line, HFWT, can greatly stimulate proliferation of CD56+ human natural killer cells and their novel precursors in blood mononuclear cells", EXPERIMENTAL HEMATOLOGY, ELSEVIER INC., US, vol. 32, no. 7, 1 July 2004 (2004-07-01), US , pages 614 - 621, XP093097015, ISSN: 0301-472X, DOI: 10.1016/j.exphem.2004.03.011
TOMCHUCK SUZANNE L.; LEUNG WING H.; DALLAS MARI H.: "Enhanced Cytotoxic Function of Natural Killer and CD3+CD56+Cells in Cord Blood after Culture", BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, KLUGE CARDEN JENNINGS PUBLISHING, CHARLOTTESVILLE, VA, US, vol. 21, no. 1, 18 October 2014 (2014-10-18), US , pages 39 - 49, XP029119353, ISSN: 1083-8791, DOI: 10.1016/j.bbmt.2014.10.014
BUI KHAC CUONG, HO VIET HOANH, NGUYEN HIEN HANH, DANG THANH CHUNG, NGO THU HANG, NGUYEN THI MAI LY, NGUYEN LINH TOAN, DANG THUY LI: "X-ray-irradiated K562 feeder cells for expansion of functional CAR-T cells", BIOCHEMISTRY AND BIOPHYSICS REPORTS, ELSEVIER, vol. 33, 1 March 2023 (2023-03-01), pages 101399, XP093097016, ISSN: 2405-5808, DOI: 10.1016/j.bbrep.2022.101399
TOMCHUCK SUZANNE L.; LEUNG WING H.; DALLAS MARI H.: "Enhanced Cytotoxic Function of Natural Killer and CD3+CD56+Cells in Cord Blood after Culture", BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, KLUGE CARDEN JENNINGS PUBLISHING, CHARLOTTESVILLE, VA, US, vol. 21, no. 1, 18 October 2014 (2014-10-18), US , pages 39 - 49, XP029119353, ISSN: 1083-8791, DOI: 10.1016/j.bbmt.2014.10.014
BUI KHAC CUONG, HO VIET HOANH, NGUYEN HIEN HANH, DANG THANH CHUNG, NGO THU HANG, NGUYEN THI MAI LY, NGUYEN LINH TOAN, DANG THUY LI: "X-ray-irradiated K562 feeder cells for expansion of functional CAR-T cells", BIOCHEMISTRY AND BIOPHYSICS REPORTS, ELSEVIER, vol. 33, 1 March 2023 (2023-03-01), pages 101399, XP093097016, ISSN: 2405-5808, DOI: 10.1016/j.bbrep.2022.101399
Attorney, Agent or Firm:
JUNGJIN IP LAW FIRM (KR)
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