Title:
METHOD FOR MEASUREMENT OF FLUORESCENCE INTENSITY OF VOLTAGE-SENSITIVE FLUORESCENT DYE
Document Type and Number:
WIPO Patent Application WO/2011/052801
Kind Code:
A1
Abstract:
Disclosed are: a method for increasing the change in voltage or ionic strength of a voltage-sensitive fluorescent dye; and the measurement of the change in action potential of a cardiomuscular cell derived from an ES cell or an iPS cell, which is never before impossible to achieve.
The screening of various substances is carried out. As a result, it is found that vitamin E has an activity of increasing the sensitivity of a voltage-sensitive fluorescent dye and cholesterol has an activity of increasing the fluorescence intensity of a voltage-sensitive fluorescent dye. It is also found that these substances can be used in combination to increase the sensitivity of a voltage-sensitive fluorescent dye with vitamin E while increasing the absolute fluorescence intensity of the voltage-sensitive fluorescent dye with cholesterol.
Inventors:
HATTORI FUMIYUKI (JP)
FUKUDA KEIICHI (JP)
SATOH YU-SUKE (JP)
FUKUDA KEIICHI (JP)
SATOH YU-SUKE (JP)
Application Number:
PCT/JP2010/069763
Publication Date:
May 05, 2011
Filing Date:
October 29, 2010
Export Citation:
Assignee:
DAIICHI SANKYO CO LTD (JP)
UNIV KEIO (JP)
HATTORI FUMIYUKI (JP)
FUKUDA KEIICHI (JP)
SATOH YU-SUKE (JP)
UNIV KEIO (JP)
HATTORI FUMIYUKI (JP)
FUKUDA KEIICHI (JP)
SATOH YU-SUKE (JP)
International Classes:
G01N21/78; G01N21/64; G01N27/416
Domestic Patent References:
| WO2006022377A1 | 2006-03-02 |
Foreign References:
| JP2003515163A | 2003-04-22 | |||
| JP2003254973A | 2003-09-10 |
Other References:
STEVEN S.-S. WANG ET AL.: "Membrane dipole potential of interaction between amyloid protein and phospholipid membranes is dependent on protein aggregation state", JOURNAL OF THE CHINESE INSTITUTE OF CHEMICAL ENGINEERS, vol. 39, 2008, pages 321 - 328, XP022682448
MARK F. VITHAA ET AL.: "Comparison of excitation and emission ratiometric fluorescence methods for quantifying the membrane dipole potential", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - BIOMEMBRANES, vol. 1768, no. 1, 2007, pages 107 - 114, XP005806225
BIOCHEM BIOPHYS RES COMMUN., vol. 385, 2009, pages 497 - 502
ADVANCES IN DRUG RESEARCH, vol. 28, 1996, pages 65 - 138
TOXICOLOGY, vol. 180, 2002, pages 151 - 167
MARK F. VITHAA ET AL.: "Comparison of excitation and emission ratiometric fluorescence methods for quantifying the membrane dipole potential", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - BIOMEMBRANES, vol. 1768, no. 1, 2007, pages 107 - 114, XP005806225
BIOCHEM BIOPHYS RES COMMUN., vol. 385, 2009, pages 497 - 502
ADVANCES IN DRUG RESEARCH, vol. 28, 1996, pages 65 - 138
TOXICOLOGY, vol. 180, 2002, pages 151 - 167
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Ono Shinjiro (JP)
Ono Shinjiro (JP)
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