Title:
METHOD FOR MEASURING BINDING AFFINITY BETWEEN TARGET AND LIGAND, AND REAGENT ASSISTANT AND KIT FOR USE IN SAID METHOD
Document Type and Number:
WIPO Patent Application WO/2015/025856
Kind Code:
A1
Abstract:
Disclosed is a method for measuring the binding affinity between a target and a ligand, and a reagent assistant and kit for use in said method. This measurement method includes a step for mixing a target and a substance capable of constituting a lipid aggregate to form a lipid aggregate containing said target, a step for bringing the target-containing lipid aggregate together with a ligand to prepare a measurement sample, and a step for measuring the amount of physical variation in the measurement sample.
Inventors:
MATSUOKA SHIGERU (JP)
MURATA MICHIO (JP)
MURATA MICHIO (JP)
Application Number:
PCT/JP2014/071682
Publication Date:
February 26, 2015
Filing Date:
August 19, 2014
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
G01N33/566; G01N21/41; G01N33/53
Foreign References:
| JP2008111713A | 2008-05-15 |
Other References:
KOHEI OTSUKA ET AL.: "Suiyosei Shibosan Mimic no Bunshi Sekkei to FABP Ketsugo Kassei", CSJ: THE CHEMICAL SOCIETY OF JAPAN KOEN YOKOSHU, vol. 93 RD, no. 3, 8 March 2013 (2013-03-08), pages 973
HIROO SASAKURA ET AL.: "Shibosan Donyu Liposome no Chosei to Bussei", JAPAN OIL CHEMISTS, vol. 41 ST, 19 September 2002 (2002-09-19), pages 172
DAISUKE MATSUOKA ET AL.: "Difference of Fatty Acid Binding Modes between Wild-type FABP3 and Its Mutants", PROTEIN SCIENCE SOCIETY OF JAPAN, vol. 13 TH, 31 May 2013 (2013-05-31), pages 65
PETER BRECHER ET AL.: "Fatty acid transfer between multilamellar liposomes and fatty acid- binding proteins", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 259, no. 21, pages 13395 - 13401
MARIA CARMEN TAN ET AL.: "Interaction kinetics of liposome-incorporated unsaturated fatty acids with fatty acid-binding protein 3 by surface plasmon resonance", BIOORG. MED. CHEM., vol. 22, no. 6, 15 March 2014 (2014-03-15), pages 1804 - 1808, XP028627442, DOI: doi:10.1016/j.bmc.2014.02.001
HIROO SASAKURA ET AL.: "Shibosan Donyu Liposome no Chosei to Bussei", JAPAN OIL CHEMISTS, vol. 41 ST, 19 September 2002 (2002-09-19), pages 172
DAISUKE MATSUOKA ET AL.: "Difference of Fatty Acid Binding Modes between Wild-type FABP3 and Its Mutants", PROTEIN SCIENCE SOCIETY OF JAPAN, vol. 13 TH, 31 May 2013 (2013-05-31), pages 65
PETER BRECHER ET AL.: "Fatty acid transfer between multilamellar liposomes and fatty acid- binding proteins", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 259, no. 21, pages 13395 - 13401
MARIA CARMEN TAN ET AL.: "Interaction kinetics of liposome-incorporated unsaturated fatty acids with fatty acid-binding protein 3 by surface plasmon resonance", BIOORG. MED. CHEM., vol. 22, no. 6, 15 March 2014 (2014-03-15), pages 1804 - 1808, XP028627442, DOI: doi:10.1016/j.bmc.2014.02.001
Attorney, Agent or Firm:
SHINDO, Takuya et al. (JP)
Takuya Shindo (JP)
Takuya Shindo (JP)
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