Title:
METHOD OF MEASURING CELL MOTILITY AND SYSTEM THEREFOR
Document Type and Number:
WIPO Patent Application WO/2007/066684
Kind Code:
A1
Abstract:
It is intended to provide a technique whereby quantitative data of cellular chemotactic
activity can be obtained. Namely, a method of measuring cell motility whereby
the motility state of cells is measured. Vital cells are located in an observation
space (100) in such a state as allowing the movements of the cells on a plane. A stimulant
affecting the cell motility is administered to the observation space (110) and
then at least one of the following factors is measured: (a) the reaction time required
from the administration of the stimulant until the movement starts for each cell;
and (b) the movement-duration time for each cell that has started the movement.
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Inventors:
NITTA NAO (JP)
Application Number:
PCT/JP2006/324350
Publication Date:
June 14, 2007
Filing Date:
December 06, 2006
Export Citation:
Assignee:
EFFECTOR CELL INST INC (JP)
NITTA NAO (JP)
NITTA NAO (JP)
International Classes:
C12Q1/02; C12M1/34
Domestic Patent References:
| WO2005104755A2 | 2005-11-10 |
Other References:
NITTA NAO ET AL.: "Quantitative analysis of eosinophil chemotaxis tracked using a novel optical device - TAXIScan", JOURNAL OF IMMUNOLOGICAL METHODS, 2007, XP003014216
TAMATANI T. ET AL.: "Atarashii Saibo Dotai Kansatsu Sochi - TAXIScan System", MEDICAL SCIENCE DIGEST, vol. 32, no. 11, 2006, pages 510 - 513, XP003014217
KANEGASAKI S. ET AL.: "A novel optical assay system for the quantitative measurement of chemotaxis", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 282, 2003, pages 1 - 11, XP004471797
LUSTER A.D. ET AL.: "Immune cell migration in inflammation: present and future therapeutic targets", NATURE IMMUNOLOGY, vol. 6, November 2005 (2005-11-01), pages 1182 - 1190, XP003014218
HAKANSSON L. ET AL.: "Migratory responses of eosinophil and neutrophil granulocytes from patients with asthma", J. ALLERGY CLIN. IMMUNOL., vol. 85, 1990, pages 743 - 750, XP003014219
FALK W. ET AL.: "Chemotaxis of purified human monocytes in vitro: Lack of accessory cell requirement", INFECTION AND IMMUNITY, vol. 36, no. 2, 1982, pages 591 - 597, XP003014220
ABRAHAM V.C. ET AL.: "High content screening applied to large-scale cell biology", TRENDS IN BIOTECHNOLOGY, vol. 22, no. 1, 2004, pages 15 - 22, XP004481953
KROOSHOOP D.J.E.B. ET AL.: "An automated multi well cell track system to study leukocyte migration", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 280, 2003, pages 89 - 102, XP004455339
TAMATANI T.: "Zoki Tokuisei - Gan no Ten'i - ni Okeru Chemokine no Yakuwari", CANCER THERAPY & HOST, vol. 17, no. 1, 2005, pages 33 - 38, XP003014221
YAMAUCHI A. ET AL.: "Aratana Saibo Sokasei Assay Method ni yori Wakatta Kochukyu no Yuso Pattern no Chigai", JAPANESE JOURNAL OF BACTERIOLOGY, vol. 60, no. 1, 2005, pages 149, XP003014222
See also references of EP 1964926A4
ABRAHAM VC; TAYLOR DL; HASKINS JR: "High content screening applied to large-scale cell biology", TRENDS BIOTECHNOL., vol. 22, no. 1, January 2004 (2004-01-01), pages 15 - 22
ANDREW D LUSTER; RONEN ALON; ULRICH H VON ANDRIAN: "Immune cell migration in inflammation: present and future therapeutic targets", NATURE IMMUNOL, vol. 6, no. 12, December 2005 (2005-12-01), pages 1182 - 1190
BOYDEN, S.: "Chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes", J. EXP. MED., vol. 115, 1962, pages 453
CUTLER, J.E.; MUNOZ, J. J.: "A simple in vitro method for studies on chemotaxis", PROC. SOC. EXP. BIOL. MED., vol. 147, 1974, pages 471
KANEGASAKI S; NOMURA Y; NITTA N; AKIYAMA S; TAMATANI T; GOSHOH Y; YOSHIDA T; SATO T; KIKUCHI Y: "A novel optical assay system for the quantitative measurement of chemotaxis", J IMMUNOL METHODS, vol. 282, no. 1-2, November 2003 (2003-11-01), pages 1 - 11
JOHN, T.J.; SIEBER, 0. F, JR.: "Chemotactic migration of neutrophils under agarose", LIFE SCI., vol. 18, 1976, pages 177
KROOSHOOP, D. J.; TORENSMA, R; VAN DEN BOSCH, G. J.; NELISSEN, J. M.; FIGDOR, C. G.; RAYMAKERS, R. A.; BOEZEMAN, J. B.: "An automated multi well cell track system to study leukocyte migration", J. IMMUNOL. METHODS, vol. 280, no. 1-2, September 2003 (2003-09-01), pages 89 - 102
NELSON, F. D.; QUIE, P. G.; SIMMONS, R. L.: "Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes", J. IMMUNOL., vol. 115, 1975, pages 1650
WICK N; THURNER S; PAIHA K; SEDIVY R; VIETOR I; HUBER LA: "Quantitative measurement of cell migration using time-lapse videomicroscopy and non-linear system analysis", HISTOCHEM CELL BIOL., vol. 119, no. 1, January 2003 (2003-01-01), pages 15 - 20
ZIGMOND S. H.: "Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors", J. CELL BIOL., vol. 75, 1977, pages 606
ZICHIA, D.; DUNN, G. A.; BROWN, G. E.: "A new direct viewing chemotaxis chamber", J. CELL SCI., vol. 99, 1991, pages 769
GRINNELL FREDERICK ET AL.: "Nested collagen matrices: A new model to study migration of human fibroblast populations in three dimensions", EXPERIMENTAL CELL RESEARCH, vol. 312, no. 1, October 2005 (2005-10-01), pages 86 - 94
TAMATANI T. ET AL.: "Atarashii Saibo Dotai Kansatsu Sochi - TAXIScan System", MEDICAL SCIENCE DIGEST, vol. 32, no. 11, 2006, pages 510 - 513, XP003014217
KANEGASAKI S. ET AL.: "A novel optical assay system for the quantitative measurement of chemotaxis", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 282, 2003, pages 1 - 11, XP004471797
LUSTER A.D. ET AL.: "Immune cell migration in inflammation: present and future therapeutic targets", NATURE IMMUNOLOGY, vol. 6, November 2005 (2005-11-01), pages 1182 - 1190, XP003014218
HAKANSSON L. ET AL.: "Migratory responses of eosinophil and neutrophil granulocytes from patients with asthma", J. ALLERGY CLIN. IMMUNOL., vol. 85, 1990, pages 743 - 750, XP003014219
FALK W. ET AL.: "Chemotaxis of purified human monocytes in vitro: Lack of accessory cell requirement", INFECTION AND IMMUNITY, vol. 36, no. 2, 1982, pages 591 - 597, XP003014220
ABRAHAM V.C. ET AL.: "High content screening applied to large-scale cell biology", TRENDS IN BIOTECHNOLOGY, vol. 22, no. 1, 2004, pages 15 - 22, XP004481953
KROOSHOOP D.J.E.B. ET AL.: "An automated multi well cell track system to study leukocyte migration", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 280, 2003, pages 89 - 102, XP004455339
TAMATANI T.: "Zoki Tokuisei - Gan no Ten'i - ni Okeru Chemokine no Yakuwari", CANCER THERAPY & HOST, vol. 17, no. 1, 2005, pages 33 - 38, XP003014221
YAMAUCHI A. ET AL.: "Aratana Saibo Sokasei Assay Method ni yori Wakatta Kochukyu no Yuso Pattern no Chigai", JAPANESE JOURNAL OF BACTERIOLOGY, vol. 60, no. 1, 2005, pages 149, XP003014222
See also references of EP 1964926A4
ABRAHAM VC; TAYLOR DL; HASKINS JR: "High content screening applied to large-scale cell biology", TRENDS BIOTECHNOL., vol. 22, no. 1, January 2004 (2004-01-01), pages 15 - 22
ANDREW D LUSTER; RONEN ALON; ULRICH H VON ANDRIAN: "Immune cell migration in inflammation: present and future therapeutic targets", NATURE IMMUNOL, vol. 6, no. 12, December 2005 (2005-12-01), pages 1182 - 1190
BOYDEN, S.: "Chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes", J. EXP. MED., vol. 115, 1962, pages 453
CUTLER, J.E.; MUNOZ, J. J.: "A simple in vitro method for studies on chemotaxis", PROC. SOC. EXP. BIOL. MED., vol. 147, 1974, pages 471
KANEGASAKI S; NOMURA Y; NITTA N; AKIYAMA S; TAMATANI T; GOSHOH Y; YOSHIDA T; SATO T; KIKUCHI Y: "A novel optical assay system for the quantitative measurement of chemotaxis", J IMMUNOL METHODS, vol. 282, no. 1-2, November 2003 (2003-11-01), pages 1 - 11
JOHN, T.J.; SIEBER, 0. F, JR.: "Chemotactic migration of neutrophils under agarose", LIFE SCI., vol. 18, 1976, pages 177
KROOSHOOP, D. J.; TORENSMA, R; VAN DEN BOSCH, G. J.; NELISSEN, J. M.; FIGDOR, C. G.; RAYMAKERS, R. A.; BOEZEMAN, J. B.: "An automated multi well cell track system to study leukocyte migration", J. IMMUNOL. METHODS, vol. 280, no. 1-2, September 2003 (2003-09-01), pages 89 - 102
NELSON, F. D.; QUIE, P. G.; SIMMONS, R. L.: "Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes", J. IMMUNOL., vol. 115, 1975, pages 1650
WICK N; THURNER S; PAIHA K; SEDIVY R; VIETOR I; HUBER LA: "Quantitative measurement of cell migration using time-lapse videomicroscopy and non-linear system analysis", HISTOCHEM CELL BIOL., vol. 119, no. 1, January 2003 (2003-01-01), pages 15 - 20
ZIGMOND S. H.: "Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors", J. CELL BIOL., vol. 75, 1977, pages 606
ZICHIA, D.; DUNN, G. A.; BROWN, G. E.: "A new direct viewing chemotaxis chamber", J. CELL SCI., vol. 99, 1991, pages 769
GRINNELL FREDERICK ET AL.: "Nested collagen matrices: A new model to study migration of human fibroblast populations in three dimensions", EXPERIMENTAL CELL RESEARCH, vol. 312, no. 1, October 2005 (2005-10-01), pages 86 - 94
Attorney, Agent or Firm:
THE PATENT CORPORATE BODY SHOWYOU INTERNATIONAL (9-10 Kitasaiwai 2-chome, Nishi-k, Yokohama-shi Kanagawa, JP)
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