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Title:
METHOD FOR MEASURING COPY NUMBER OF EXOGENOUS ANTIBODY GENES AND KIT
Document Type and Number:
WIPO Patent Application WO/2019/128857
Kind Code:
A1
Abstract:
Provided are a method for measuring the copy number of exogenous antibody genes, a kit, and a primer pair. Specifically, provided is a primer pair, comprising: primer pair 1, and a primer pair as shown in SEQ ID NO:1 and SEQ ID NO:2, and preferably, further comprising primer pair 2, and a primer pair as shown in SEQ ID NO:4 and SEQ ID NO:5. Also provided is a method for measuring the copy number of exogenous antibody genes, so as to provide a more accurate technical support for the evaluation of transduction efficiency, quality control, and in vitro monitoring for the exogenous antibody genes. The detection of the exogenous antibody genes containing an IgG4 heavy chain constant region is universal.

Inventors:
QIAN QIJUN (CN)
JIN HUAJUN (CN)
HAO FANGYUAN (CN)
WANG CHAO (CN)
SUN JUANJUAN (CN)
Application Number:
PCT/CN2018/122561
Publication Date:
July 04, 2019
Filing Date:
December 21, 2018
Export Citation:
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Assignee:
SHANGHAI CELL THERAPY RES INST (CN)
SHANGHAI CELL THERAPY GROUP CO LTD (CN)
International Classes:
C12Q1/6851
Foreign References:
CN105331585A2016-02-17
CN104780939A2015-07-15
Other References:
TAYLOR, L. ET AL.: "In Vitro and in Vivo Activities of OX40 ( CD 134)-IgG Fusion Protein Isoforms with Different Levels of Immune-Effector Functions", JOURNAL OF LEUKOCYTE BIOLOGY., vol. 72, no. 3, 30 September 2002 (2002-09-30), pages 522 - 529, XP009040357
SUZUKI, T. ET AL.: "Signals Mediated by FcyRIIA Suppress the Growth of B-Lineage Acute Lymphoblastic Leukemia Cells", LEUKEMIA., vol. 16, no. 7, July 2002 (2002-07-01), pages 1276 - 1284, XP055622420
AHMAD, M. ET AL.: "Individuals with IgG4-Related Disease do not Have an Increased Frequency of the K409 Variant of IgG4 that Compromises Fab-Arm Exchange", J RHEUMATOL., vol. 41, no. 1, 31 January 2014 (2014-01-31), pages 185 - 187, XP055622422
Attorney, Agent or Firm:
CCPIT PATENT AND TRADEMARK LAW OFFICE (CN)
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