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Title:
METHOD FOR MEASURING DEGREE OF DEACETYLATION OF CHITOSAN OLIGOSACCHARIDE BY USING FIRST-ORDER DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY
Document Type and Number:
WIPO Patent Application WO/2018/040821
Kind Code:
A1
Abstract:
A method for measuring the degree of deacetylation of a chitosan oligosaccharide by using first-order derivative ultraviolet spectrophotometry, comprising the steps of 1) with a hydrochloric acid solution having a concentration of 0.3-1.0 mol/L as a blank solvent, preparing standard solutions of N-acetyl-D-glucosamine at a series of concentrations, measuring the ultraviolet absorbance values A of the standard solutions of N-acetyl-D-glucosamine within a wavelength range of 200-206 nm, and plotting a standard curve according to the concentrations of N-acetyl-D-glucosamine and ΔA/Δλ; and 2) dissolving a chitosan oligosaccharide sample with the blank solvent, with the blank solvent as a reference, measuring the absorbance value of the chitosan oligosaccharide sample at a wavelength of 200-206 nm, and calculating the degree of deacetylation of the chitosan oligosaccharide sample according to the standard curve.

Inventors:
SU, Zhengquan (No. 40, Guanghanzhi Street Baogang Avenue, Haizhu Distric, Guangzhou Guangdong 4, 510224, CN)
GUO, Jiao (No. 40, Guanghanzhi Street Baogang Avenue, Haizhu Distric, Guangzhou Guangdong 4, 510224, CN)
JIANG, Yao (No. 40, Guanghanzhi Street Baogang Avenue, Haizhu Distric, Guangzhou Guangdong 4, 510224, CN)
Application Number:
CN2017/095114
Publication Date:
March 08, 2018
Filing Date:
July 31, 2017
Export Citation:
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Assignee:
GUANGDONG PHARMACEUTICAL UNIVERSITY (No. 40, Guanghanzhi Street Baogang Avenue, Haizhu Distric, Guangzhou Guangdong 4, 510224, CN)
International Classes:
G01N21/33
Foreign References:
CN106248607A2016-12-21
CN103983593A2014-08-13
Other References:
ZHU, XIAOJING: "Study the Methods on Determination of the Degree )f Deacetylation of Chitosan", CMFD ENGINEERING TECHNOLOGY I., 15 March 2012 (2012-03-15)
LIU, CHANGXIA: "An Improved Preparation Method for Chitosan, Carboxymethylchitosan and Chitosan Salts and Their Quality Analysis", CMFD ENGINEERING TECHNOLOGY I., 15 September 2003 (2003-09-15), pages 32 - 38
LIU, CHANGXIA ET AL.: "First Derivative UV-Spectrophotometric Determination of the Degree of Deacetylation of Chitosan", PHYSICAL TESTING AND CHEMICAL ANALYSIS PART B CHEMICAL ANALYSIS, vol. 39, no. 4, 30 April 2003 (2003-04-30), pages 195 - 197
RICARDO M. P. DA SILVA ET AL.: "Straightforward Determination of the Degree of N-Acetylation of Chitosan by Means of First-Derivative UV Spectrophotome-try", MACROMOLECULAR CHEMISTRY AND PHYSICS, vol. 209, no. 14, 31 December 2008 (2008-12-31), pages 1463 - 1472, XP055471396, Retrieved from the Internet
Attorney, Agent or Firm:
JJS PATENT ATTORNEYS (LIU Ye, Room 1510 Xueyuan International Tower, No. 1 Zhichun Road, Haidian District, Beijing 1, 100191, CN)
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