Title:
METHOD FOR MEASURING DEGREE OF DEACETYLATION OF CHITOSAN OLIGOSACCHARIDE BY USING FIRST-ORDER DERIVATIVE ULTRAVIOLET SPECTROPHOTOMETRY
Document Type and Number:
WIPO Patent Application WO/2018/040821
Kind Code:
A1
Abstract:
A method for measuring the degree of deacetylation of a chitosan oligosaccharide by using first-order derivative ultraviolet spectrophotometry, comprising the steps of 1) with a hydrochloric acid solution having a concentration of 0.3-1.0 mol/L as a blank solvent, preparing standard solutions of N-acetyl-D-glucosamine at a series of concentrations, measuring the ultraviolet absorbance values A of the standard solutions of N-acetyl-D-glucosamine within a wavelength range of 200-206 nm, and plotting a standard curve according to the concentrations of N-acetyl-D-glucosamine and ΔA/Δλ; and 2) dissolving a chitosan oligosaccharide sample with the blank solvent, with the blank solvent as a reference, measuring the absorbance value of the chitosan oligosaccharide sample at a wavelength of 200-206 nm, and calculating the degree of deacetylation of the chitosan oligosaccharide sample according to the standard curve.
Inventors:
SU ZHENGQUAN (CN)
GUO JIAO (CN)
JIANG YAO (CN)
GUO JIAO (CN)
JIANG YAO (CN)
Application Number:
PCT/CN2017/095114
Publication Date:
March 08, 2018
Filing Date:
July 31, 2017
Export Citation:
Assignee:
UNIV GUANGDONG PHARM (CN)
International Classes:
G01N21/33
Foreign References:
| CN106248607A | 2016-12-21 | |||
| CN103983593A | 2014-08-13 |
Other References:
ZHU, XIAOJING: "Study the Methods on Determination of the Degree )f Deacetylation of Chitosan", CMFD ENGINEERING TECHNOLOGY I., 15 March 2012 (2012-03-15)
LIU, CHANGXIA: "An Improved Preparation Method for Chitosan, Carboxymethylchitosan and Chitosan Salts and Their Quality Analysis", CMFD ENGINEERING TECHNOLOGY I., 15 September 2003 (2003-09-15), pages 32 - 38
LIU, CHANGXIA ET AL.: "First Derivative UV-Spectrophotometric Determination of the Degree of Deacetylation of Chitosan", PHYSICAL TESTING AND CHEMICAL ANALYSIS PART B CHEMICAL ANALYSIS, vol. 39, no. 4, 30 April 2003 (2003-04-30), pages 195 - 197
RICARDO M. P. DA SILVA ET AL.: "Straightforward Determination of the Degree of N-Acetylation of Chitosan by Means of First-Derivative UV Spectrophotome-try", MACROMOLECULAR CHEMISTRY AND PHYSICS, vol. 209, no. 14, 31 December 2008 (2008-12-31), pages 1463 - 1472, XP055471396, Retrieved from the Internet
LIU, CHANGXIA: "An Improved Preparation Method for Chitosan, Carboxymethylchitosan and Chitosan Salts and Their Quality Analysis", CMFD ENGINEERING TECHNOLOGY I., 15 September 2003 (2003-09-15), pages 32 - 38
LIU, CHANGXIA ET AL.: "First Derivative UV-Spectrophotometric Determination of the Degree of Deacetylation of Chitosan", PHYSICAL TESTING AND CHEMICAL ANALYSIS PART B CHEMICAL ANALYSIS, vol. 39, no. 4, 30 April 2003 (2003-04-30), pages 195 - 197
RICARDO M. P. DA SILVA ET AL.: "Straightforward Determination of the Degree of N-Acetylation of Chitosan by Means of First-Derivative UV Spectrophotome-try", MACROMOLECULAR CHEMISTRY AND PHYSICS, vol. 209, no. 14, 31 December 2008 (2008-12-31), pages 1463 - 1472, XP055471396, Retrieved from the Internet
Attorney, Agent or Firm:
JJS PATENT ATTORNEYS (CN)
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