Title:
METHOD FOR MEASURING IMMUNOGENICITY OF PROTEIN AGENT
Document Type and Number:
WIPO Patent Application WO/2018/044086
Kind Code:
A2
Abstract:
The present invention relates to a method for measuring immunogenicity of a protein agent, the method comprising the steps of: constructing a library of peripheral blood mononuclear cells having various HLA-DRB1 genotypes; culturing peripheral blood mononuclear cell CD14+ monocyte-derived immature dendritic cells by genotype in a medium containing a protein to be measured, GM-CSF, IL-4, TNF-α, IL-1β, IL-6, and PGE2 to induce mature dendritic cells; removing CD8+ T cells from peripheral blood mononuclear cells according to genotypes to prepare CD8+ T cell-removed peripheral blood mononuclear cells; co-culturing the mature dendritic cells and the CD8+ T cell-removed peripheral blood mononuclear cells at a cell count ratio of 1:5 to 1:20; and quantitatively analyzing the CD4+ T cells proliferated by co-cultivation according to genotypes, whereby the immunogenicity of a protein agent under development can be predicted with high accuracy before a clinical phase, thus increasing the development efficiency of protein agents.
Inventors:
LIM OK JAE (KR)
SHIN DUCK HYANG (KR)
SHIN DUCK HYANG (KR)
Application Number:
PCT/KR2017/009522
Publication Date:
March 08, 2018
Filing Date:
August 31, 2017
Export Citation:
Assignee:
MOGAM INST BIOMEDICAL RES (KR)
Attorney, Agent or Firm:
DOOHO IP LAW FIRM (KR)
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