Title:
METHOD FOR MEASURING INTRACELLULAR POTENTIAL BY CAPACITANCE TYPE POTENTIAL MEASUREMENT DEVICE
Document Type and Number:
WIPO Patent Application WO/2019/208828
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for accurately measuring and controlling intracellular potential by a simple method that is less invasive to the cell and does not require a skilled technique. The method according to the present invention comprises: by using magnetic force from a magnetic electrode adhered to the surface of a target cell, into which conductive nanoparticles have been preliminarily introduced, or a magnet being in contact with a conductive plate electrode, attracting the conductive nanoparticles inside the cell to the side of the cell surface adhering to the electrode, and then allowing the conductive nanoparticles to pass through the cell membrane to thereby bring the end outside the cell into contact with the magnetic electrode or the conductive plate electrode; or alternatively, adhering the conductive nanoparticles adsorbed on the magnetic electrode surface to the upper side of the target cell, and allowing the conductive nanoparticles to pass through the cell membrane by attracting the conductive nanoparticles to an iron plate disposed on the lower side of the cell to thereby leave the end outside the cell being in contact with the magnetic electrode. A capacitor is fabricated by using the conductive plate in contact with the conductive nanoparticles together with a conductive plate (an aluminum foil). Alternatively, a capacitor is fabricated by disposing a magnetic body via an insulating body on the upper side of the magnetic electrode. This capacitor detects, as a sensor, a change in charge inside the target cell and converts the same into a voltage change to thereby enable the measurement of the intracellular potential. The present invention also makes it possible to monitor the effect of a test reagent, said test reagent being administered to an extracellular solution, on a cell.
Inventors:
SAITO MITSUYOSHI (JP)
Application Number:
PCT/JP2019/018158
Publication Date:
October 31, 2019
Filing Date:
April 26, 2019
Export Citation:
Assignee:
ION CHAT RES CORPORATE (JP)
SAITO MITSUYOSHI (JP)
SAITO MITSUYOSHI (JP)
International Classes:
C12M1/42; C12M1/34; C12Q1/06; G01N27/00; G01N27/28; G01N33/15; G01N33/483; G01N33/50
Domestic Patent References:
| WO2018199334A1 | 2018-11-01 | |||
| WO2012043820A1 | 2012-04-05 | |||
| WO2013061849A1 | 2013-05-02 | |||
| WO2014098182A1 | 2014-06-26 | |||
| WO2014192312A1 | 2014-12-04 |
Foreign References:
| US20100330612A1 | 2010-12-30 | |||
| JP2005505761W | ||||
| US9663764B2 | 2017-05-30 | |||
| JP2006217866A | 2006-08-24 |
Other References:
ANNA FENDYUR ET AL., FRONTIERS IN NEUROENGINEERING, vol. 4, December 2011 (2011-12-01), pages 1 - 14
RAPHAEL LEVY ET AL., NANOREVIEWS, vol. 1, 2010, pages 4889
MICHA E. SPIRA ET AL., NATURE NANOTECHNOLOGY, vol. 8, February 2013 (2013-02-01), pages 83 - 94
KHUDHAIR D. ET AL., EMERGING TRENDS IN NEURO ENGINEERING AND NEURAL COMPUTATION, 2017, pages 41 - 59
YAMADA ET AL., WIRES NANOMED NANOBIOTECHNOL, vol. 7, 2015, pages 428 - 445
RAMESANJAYAKRISHNAN, SPE PLASTIC RESEARCH, 2017
OO OTELAJAD.-H. HAT. LYH. ZHANGRD ROBINSON: "Highly Conductive Cu2-xS Nanoparticle Films, which have been conventionally used for staining of biological imaging through Room Temperature Processing and an Order of Magnitude Enhancement of Conductivity via Electrophoretic Deposition", ACS APPLIED MATERIALS AND INTERFACES, vol. 6, 2014, pages 18911 - 18920
MOSCHO ET AL., PNAS, vol. 93, 1996, pages 11443 - 11447
SCHLESINGER SAITO, CELL DEATH. AND DIFFERENTIATION, vol. 13, 2006, pages 1403 - 1408
AIMON ET AL., PLOS ONE, vol. 6, no. 10, 2011, pages e25529
LEVY ET AL.: "Gold nanoparticles delivery in mammalian live cells: a critical review", NANO REVIEWS, vol. 1, 2010, pages 4889
WALEV ET AL., PNAS, vol. 98, no. 6, 2001, pages 3185 - 3190
T. TOMITA., BIO.SOCI. JAPAN GENERAL INCORPORATED. ASSOCIATION, vol. 34, no. 6, 1994, pages 6 - 11
ROSABELOTI: "Effect of cpTi Surface Roughness on Human Bone Marrow Cell Attachment, Proliferation, and Differentiation", BRAZ DENT J, vol. 14, no. 1, 2003, pages 16 - 21
WANG ET AL., J. OF BIOL. CHEM., vol. 284, no. 9, 2009, pages 5685 - 5696
See also references of EP 3786279A4
RAPHAEL LEVY ET AL., NANOREVIEWS, vol. 1, 2010, pages 4889
MICHA E. SPIRA ET AL., NATURE NANOTECHNOLOGY, vol. 8, February 2013 (2013-02-01), pages 83 - 94
KHUDHAIR D. ET AL., EMERGING TRENDS IN NEURO ENGINEERING AND NEURAL COMPUTATION, 2017, pages 41 - 59
YAMADA ET AL., WIRES NANOMED NANOBIOTECHNOL, vol. 7, 2015, pages 428 - 445
RAMESANJAYAKRISHNAN, SPE PLASTIC RESEARCH, 2017
OO OTELAJAD.-H. HAT. LYH. ZHANGRD ROBINSON: "Highly Conductive Cu2-xS Nanoparticle Films, which have been conventionally used for staining of biological imaging through Room Temperature Processing and an Order of Magnitude Enhancement of Conductivity via Electrophoretic Deposition", ACS APPLIED MATERIALS AND INTERFACES, vol. 6, 2014, pages 18911 - 18920
MOSCHO ET AL., PNAS, vol. 93, 1996, pages 11443 - 11447
SCHLESINGER SAITO, CELL DEATH. AND DIFFERENTIATION, vol. 13, 2006, pages 1403 - 1408
AIMON ET AL., PLOS ONE, vol. 6, no. 10, 2011, pages e25529
LEVY ET AL.: "Gold nanoparticles delivery in mammalian live cells: a critical review", NANO REVIEWS, vol. 1, 2010, pages 4889
WALEV ET AL., PNAS, vol. 98, no. 6, 2001, pages 3185 - 3190
T. TOMITA., BIO.SOCI. JAPAN GENERAL INCORPORATED. ASSOCIATION, vol. 34, no. 6, 1994, pages 6 - 11
ROSABELOTI: "Effect of cpTi Surface Roughness on Human Bone Marrow Cell Attachment, Proliferation, and Differentiation", BRAZ DENT J, vol. 14, no. 1, 2003, pages 16 - 21
WANG ET AL., J. OF BIOL. CHEM., vol. 284, no. 9, 2009, pages 5685 - 5696
See also references of EP 3786279A4
Attorney, Agent or Firm:
SAEKI, Takuro et al. (JP)
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