METHOD OF MEASURING LUMINESCENCE DOSE AT DEFINITE SITE, APPARATUS FOR MEASURING LUMINESCENCE DOSE AT DEFINITE SITE, METHOD OF MEASURING EXPRESSION AMOUNT AND MEASUREMENT APPARATUS THEREFOR

Title:

METHOD OF MEASURING LUMINESCENCE DOSE AT DEFINITE SITE, APPARATUS FOR MEASURING LUMINESCENCE DOSE AT DEFINITE SITE, METHOD OF MEASURING EXPRESSION AMOUNT AND MEASUREMENT APPARATUS THEREFOR

Document Type and Number:

WIPO Patent Application WO/2006/106882

Kind Code:

A1

Abstract:

It is intended to provide a method of measuring a luminescence dose at a definite site, which makes it possible, in measuring a luminescence dose from a definite site in a vital sample, to confirm whether or not a luminescent protein at the definite site is localized in the same sample as the above-described one, an apparatus for measuring a luminescence dose at a definite site and so on. An apparatus (100) for measuring a luminescence dose at a definite site comprises a sample (102) having a fused gene, which has a fluorescence-related gene expressing a fluorescent protein in addition to a shift base sequence and a luminescence-related gene fused together, transferred thereinto, a container (103) in which the sample (102) is contained, a stage (104) on which the container (103) is located, a fluorescent image pick-up unit (106) (an objective lens (106a) to a CCD camera (106c), and an image-forming lens (106f)) for picking up a luminescent image of the sample (102), a fluorescent image pick-up unit (108) (an objective lens (108a) to a shutter (108f)) for picking up a fluorescent image of the sample (102), and an information and communication terminal (110).

Inventors:

SUZUKI HIROBUMI (JP)
OHASHI YOKO (JP)
KAWASAKI KENJI (JP)
KOJIMA KIYOTSUGU (JP)
KOYAMA KENICHI (JP)
YOSHIKAWA AKIHIKO (JP)

Application Number:

PCT/JP2006/306755

Publication Date:

October 12, 2006

Filing Date:

March 30, 2006

Export Citation:

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Assignee:

OLYMPUS CORP (JP)
SUZUKI HIROBUMI (JP)
OHASHI YOKO (JP)
KAWASAKI KENJI (JP)
KOJIMA KIYOTSUGU (JP)
KOYAMA KENICHI (JP)
YOSHIKAWA AKIHIKO (JP)

International Classes:

C12Q1/68; C12N15/00; G02B21/18; G02B21/36

Foreign References:

JP2001501100A	2001-01-30
JP2003180393A	2003-07-02
FR2840320A1	2003-12-05

Other References:

KENNEDY H.J. ET AL.: "Glucose Generates Sub-plasma Membrane ATP Microdomains in Single Islet beta-Cells. POTENTIAL ROLE FOR STRATEGICALLY LOCATED MITOCHONDRIA", J. BIOL. CHEM., vol. 274, 1999, pages 13281 - 13291, XP003006106
MULLANEY J. M. ET AL.: "Activity of foreign proteins targeted within the bacteriophage T4 head and prohead: implications for packaged DNA structure", JOURNAL OF MOLECULAR BIOLOGY, vol. 283, no. 5, 13 November 1998 (1998-11-13), pages 913 - 929, XP004462337, DOI: doi:10.1006/jmbi.1998.2126
MOLINA, A. ET AL.: "A transformed fish cell line expressing a green fluorescent protein- luciferase fusion gene responding to cellular stress", TOXICOLOGY IN VITRO, vol. 16, no. 2, April 2002 (2002-04-01), pages 201 - 207, XP002557950, DOI: doi:10.1016/S0887-2333(01)00106-0
PNONMAREV, VLADIMIR ET AL.: "A novel triple-modality reporter gene for whole-body fluorescent, bioluminescent, and nuclear non-invasive imaging", EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING, May 2004 (2004-05-01)
See also references of EP 1865073A4

Attorney, Agent or Firm:

Sakai, Hiroaki (Kasumigaseki Building 2-5, Kasumigaseki 3-chome, Chiyoda-k, Tokyo 19, JP)

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