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Title:
METHOD FOR MEASURING PHYSIOLOGICALLY ACTIVE SUBSTANCE OF BIOLOGICAL ORIGIN
Document Type and Number:
WIPO Patent Application WO/2013/069749
Kind Code:
A1
Abstract:
Provided is a technique which enables the detection of a physiologically active substance of biological origin or the measurement of the concentration of the physiologically active substance with higher accuracy and also enables the visual observation of the process of the aggregation or gelatinization of a protein, which is caused by the reaction between an AL and the physiologically active substance in a method for measuring the physiologically active substance. A method for measuring a specific physiologically active substance of biological origin, comprising preparing a mixed solution of an AL reagent and a sample containing the physiologically active substance and detecting the aggregation or gelatinization of a protein in the mixed solution while agitating the mixed solution, thereby detecting the physiologically active substance or measuring the concentration of the physiologically active substance in the sample. A function of emitting a predetermined fluorescence is imparted to a substance involved in the aggregation or gelatinization in the sample to be measured and/or the AL reagent, and the fluorescence emitted from the substance involved in the aggregation or gelatinization is measured or observed in the mixed solution. In this manner, the aggregation or gelatinization in the mixed solution can be detected.

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Inventors:
YABUSAKI KATSUMI (JP)
Application Number:
PCT/JP2012/079037
Publication Date:
May 16, 2013
Filing Date:
November 08, 2012
Export Citation:
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Assignee:
KOWA CO (JP)
International Classes:
G01N33/579; G01N21/64; G01N21/82
Domestic Patent References:
WO2008038329A12008-04-03
Foreign References:
JP2009150723A2009-07-09
JP2009521221A2009-06-04
JP2006512077A2006-04-13
JP2009150723A2009-07-09
JP2009118854A2009-06-04
Other References:
See also references of EP 2778682A4
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
Yoshiyuki Kawaguchi (JP)
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Claims:



 
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