Title:
METHOD FOR MEASURING SINGLE-MOLECULE RNA FORCE SPECTRUM AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/067478
Kind Code:
A1
Abstract:
The present invention provides a method for measuring a single-molecule RNA force spectrum and a use thereof. The method comprises the following steps: 1) obtaining a handle chain: designing and synthesizing a specially modified handle chain primer, and obtaining a specially modified handle chain according to the handle chain primer, the handle chain comprising a handle chain 1 and a handle chain 2, and the handle chain 1 and the handle chain 2 having cohesive ends; and 2) obtaining a single-molecule RNA-handle composite chain: obtaining a single-molecule RNA, the RNA having a fragment complementarily paired with the cohesive end, and annealing the handle chain and the RNA to obtain a single-molecule RNA-handle composite chain. The present method can rapidly, efficiently and accurately carry out force spectrum measurement on a single RNA molecule. The single-molecule RNA composite chain prepared by the method can bear greater acting force, can be used for measurement of higher acting forces, and can also be used for RNA structure analysis.
Inventors:
SHI HANG (CN)
KANG YAFENG (CN)
KANG YAFENG (CN)
Application Number:
PCT/CN2023/121087
Publication Date:
April 04, 2024
Filing Date:
September 25, 2023
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
C12Q1/6806; C12N15/11; C12Q1/6876
Foreign References:
| CN116287109A | 2023-06-23 | |||
| US20220148683A1 | 2022-05-12 |
Other References:
XINYAO HU: "Optical Tweezers Study of RpoS RNA Auto-inhibitory Stem-loop", CHINESE DOCTORAL DISSERTATIONS FULL-TEXT DATABASE (BASIC SCIENCES), UNIVERSITY OF SCIENCE AND TECHOLOGY OF CHINA, 15 August 2019 (2019-08-15), University of Science and Techology of China, XP093151440, [retrieved on 20240415]
MANGEOL P., CÔTE D., BIZEBARD T., LEGRAND O., BOCKELMANN U.: "Probing DNA and RNA single molecules with a double optical tweezer", THE EUROPEAN PHYSICAL JOURNAL E, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 19, no. 3, 17 February 2006 (2006-02-17), Berlin/Heidelberg, pages 311 - 317, XP093151443, ISSN: 1292-8941, DOI: 10.1140/epje/i2005-10060-4
YIN-MEI LI, WANG HAO-WEI, GONG LEI: "Current applied researches of optical tweezers in biology", JOURNAL OF BIOLOGY, vol. 36, no. 3, 13 May 2019 (2019-05-13), pages 1 - 8, XP093151448
BUSTAMANTE CARLOS J., CHEMLA YANN R., LIU SHIXIN, WANG MICHELLE D.: "Optical tweezers in single-molecule biophysics", NATURE REVIEWS METHODS PRIMERS, vol. 1, no. 1, 25 March 2021 (2021-03-25), pages 25, XP093151433, ISSN: 2662-8449, DOI: 10.1038/s43586-021-00021-6
MANGEOL P., CÔTE D., BIZEBARD T., LEGRAND O., BOCKELMANN U.: "Probing DNA and RNA single molecules with a double optical tweezer", THE EUROPEAN PHYSICAL JOURNAL E, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 19, no. 3, 17 February 2006 (2006-02-17), Berlin/Heidelberg, pages 311 - 317, XP093151443, ISSN: 1292-8941, DOI: 10.1140/epje/i2005-10060-4
YIN-MEI LI, WANG HAO-WEI, GONG LEI: "Current applied researches of optical tweezers in biology", JOURNAL OF BIOLOGY, vol. 36, no. 3, 13 May 2019 (2019-05-13), pages 1 - 8, XP093151448
BUSTAMANTE CARLOS J., CHEMLA YANN R., LIU SHIXIN, WANG MICHELLE D.: "Optical tweezers in single-molecule biophysics", NATURE REVIEWS METHODS PRIMERS, vol. 1, no. 1, 25 March 2021 (2021-03-25), pages 25, XP093151433, ISSN: 2662-8449, DOI: 10.1038/s43586-021-00021-6
Attorney, Agent or Firm:
BEIJING SANYOU INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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