Title:
METHOD FOR MINUTELY REGULATING GENE EXPRESSION USING SYNTHESIS-REGULATING SRNA
Document Type and Number:
WIPO Patent Application WO/2015/190627
Kind Code:
A1
Abstract:
The present invention relates to a method for minutely regulating gene expression using a synthesis-regulating sRNA in a prokaryotic cell and, more specifically, to a synthesis-regulating sRNA capable of minutely regulating the expression of a target gene by regulating the level of sRNA expression or the binding force between the sRNA and Hfq so as to minutely regulate the expression of the target gene. The method for minutely regulating gene expression using a synthesis-regulating sRNA, according to the present invention, can minutely regulate the degree of inhibiting the expression of a target gene by regulating the level of sRNA expression or the binding force between the sRNA and Hfq and thus can simultaneously, easily, and quickly apply various target gene combinations to various strains without gene deletion through the synthesis-regulating sRNA for regulating gene expression and is therefore very suitable for measuring the metabolizability of each strain and selecting an optimum strain. In addition, the method for minutely regulating gene expression using a synthesis-regulating sRNA has the advantages of easily and quickly selecting a target gene for the inhibition of gene expression and expressing the gene thus selected to a desired degree and thus can be used in producing recombinant strains for the efficient production of various metabolites and establishing a method for the efficient production and is therefore very useful.
Inventors:
LEE SANG YUP (KR)
ROH MINHO (KR)
YOO SEUNG MIN (KR)
ROH MINHO (KR)
YOO SEUNG MIN (KR)
Application Number:
PCT/KR2014/005120
Publication Date:
December 17, 2015
Filing Date:
June 11, 2014
Export Citation:
Assignee:
KOREA ADVANCED INST SCI & TECH (KR)
International Classes:
C12N15/09; C12N1/20
Foreign References:
| KR20130082474A | 2013-07-19 | |||
| US20100311137A1 | 2010-12-09 | |||
| US20060057607A1 | 2006-03-16 | |||
| KR20120140185A | 2012-12-28 |
Other References:
SO, LOK YAN ET AL.: "pZM07-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis", BMC MICROBIOL., vol. 14, no. 68, 15 March 2014 (2014-03-15), pages 1 - 16, XP021182848
Attorney, Agent or Firm:
LEE, Cheo Young et al. (KR)
이처영 (KR)
이처영 (KR)
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