Title:
A METHOD FOR MIXED MODE ADSORPTION AND MIXED MODE ADSORBENTS
Document Type and Number:
WIPO Patent Application WO2002053252
Kind Code:
A3
Abstract:
Method for the removal of a substance from an aqueous liquid by ion exchange comprising providing a liquid with said substance is present; providing an adsorption matrix which comprises at least two different ligands; contacting the liquid with the matrix under a period of time and conditions sufficient to allow adsorption of the substance to the matrix; and adding an eluent that desorbs the substance from the matrix. Each one of the ligands interacts with the substance during the adsorption step, and at least one of the ligands is charged and capable of ionic interaction with the substance. The method can be run as a cation or anion exchange. The substance desorption can be performed by adding an eluent comprising an increasing ionic strength.The invention also encompasses an adsorbent comprising at least two structurally different ligands, which interact with the same kind of substance when used in a separation procedure.
Inventors:
BELEW MAKONNEN (SE)
JOHANSSON BO-LENNART (SE)
MALOISEL JEAN-LUC (SE)
JOHANSSON BO-LENNART (SE)
MALOISEL JEAN-LUC (SE)
Application Number:
PCT/EP2001/014895
Publication Date:
September 26, 2002
Filing Date:
December 17, 2001
Export Citation:
Assignee:
AMERSHAM BIOSCIENCES AB (SE)
BELEW MAKONNEN (SE)
JOHANSSON BO-LENNART (SE)
MALOISEL JEAN-LUC (SE)
BELEW MAKONNEN (SE)
JOHANSSON BO-LENNART (SE)
MALOISEL JEAN-LUC (SE)
International Classes:
B01D15/04; B01D15/08; B01J20/26; B01J20/34; B01J39/04; B01D15/00; B01J39/26; B01J41/04; B01J41/20; B01J43/00; B01J47/00; B01J49/00; C07K1/18; (IPC1-7): B01J39/06; B01J41/06; B01J39/04; B01J41/04; B01J43/00
Domestic Patent References:
| WO2001038228A1 | 2001-05-31 | |||
| WO2000069872A2 | 2000-11-23 | |||
| WO1999065607A1 | 1999-12-23 | |||
| WO1996009116A1 | 1996-03-28 |
Foreign References:
| US6090288A | 2000-07-18 | |||
| EP0276138A2 | 1988-07-27 |
Other References:
BISCHOFF R ET AL: "ISOLATION OF SPECIFIC TRNAS USING AN IONIC-HYDROPHOBIC MIXED-MODE CHROMATOGRAPHIC MATRIX", ANALYTICAL BIOCHEMISTRY, ORLANDO, FL, US, vol. 151, 1985, pages 526 - 533, XP001000887, ISSN: 0003-2697
MCLAUGHLIN L W: "Mixed-mode chromatography of nucleic acids", CHEMICAL REVIEWS, AMERICAN CHEMICAL SOCIETY. EASTON, US, vol. 89, 1989, pages 309 - 319, XP002152137, ISSN: 0009-2665
MCLAUGHLIN L W: "Mixed-mode chromatography of nucleic acids", CHEMICAL REVIEWS, AMERICAN CHEMICAL SOCIETY. EASTON, US, vol. 89, 1989, pages 309 - 319, XP002152137, ISSN: 0009-2665
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