Title:
METHOD FOR MONITORING POST-TRANSLATIONAL MODIFICATION OF PROTEINS
Document Type and Number:
WIPO Patent Application WO/2018/143531
Kind Code:
A1
Abstract:
A disclosed method for monitoring post-translational modification of proteins comprises the following steps: preparing a first micro bead by binding a protein antibody to the surface of a base bead; preparing a second micro bead by binding a protein of interest having a first post-translational modification or a second post-translational modification, which are inversely proportional to each other, to the protein antibody of the first micro bead; preparing a third micro bead by binding the second micro bead to a first post-translationally modified antibody that selectively binds to the first post-translational modification of the protein of interest; preparing a fourth micro bead by binding the second micro bead to a second post-translationally modified antibody that selectively binds to the second post-translational modification of the protein of interest; measuring impedances of the third micro bead and the fourth micro bead, respectively; and obtaining a ratio of a first difference between the impedance of the third micro bead and a reference impedance to a second difference between the impedance of the fourth micro bead and the reference impedance.
Inventors:
LEE SOO-HYUN (KR)
KANG JI-YOON (KR)
KIM MIN-HO (KR)
KIM YUN-KYUNG (KR)
KANG JI-YOON (KR)
KIM MIN-HO (KR)
KIM YUN-KYUNG (KR)
Application Number:
PCT/KR2017/009793
Publication Date:
August 09, 2018
Filing Date:
September 07, 2017
Export Citation:
Assignee:
KOREA INST SCI & TECH (KR)
International Classes:
G01N33/68; G01N27/02; G01N33/543
Domestic Patent References:
| WO2016193980A1 | 2016-12-08 |
Foreign References:
| US20100331200A1 | 2010-12-30 | |||
| US20060223130A1 | 2006-10-05 |
Other References:
DOLL, S.: "Mass spectrometry-based detection and assignment of protein posttranslational modifications", ACS CHEMICAL BIOLOGY, vol. 10, no. 1, 16 January 2015 (2015-01-16), pages 63 - 71, XP055530845
ESTEVES-VILLANUEVA, J. 0.: "Electrochemical detection of anti-tau antibodies binding to tau protein and inhibition of GSK-3β-catalyzed phosphorylation", ANALYTICAL BIOCHEMISTRY, vol. 496, March 2016 (2016-03-01), pages 55 - 62, XP029407009
HUNDELT MSHAHANI N: "Tau alteration and neuronal degeneration in tauopathies: mechanisms and models", BIOCHIMICA. ET BIOPHYSICA. ACTA, vol. 1739, 2005, pages 331 - 354, XP025329114, doi:10.1016/j.bbadis.2004.06.018
GENDRON TFPETRUCELLI L: "The role of tau in neurodegeneration", MOL. NEURODEGENER., vol. 4, 2009, pages 13, XP021052326, doi:10.1186/1750-1326-4-13
See also references of EP 3578986A4
ESTEVES-VILLANUEVA, J. 0.: "Electrochemical detection of anti-tau antibodies binding to tau protein and inhibition of GSK-3β-catalyzed phosphorylation", ANALYTICAL BIOCHEMISTRY, vol. 496, March 2016 (2016-03-01), pages 55 - 62, XP029407009
HUNDELT MSHAHANI N: "Tau alteration and neuronal degeneration in tauopathies: mechanisms and models", BIOCHIMICA. ET BIOPHYSICA. ACTA, vol. 1739, 2005, pages 331 - 354, XP025329114, doi:10.1016/j.bbadis.2004.06.018
GENDRON TFPETRUCELLI L: "The role of tau in neurodegeneration", MOL. NEURODEGENER., vol. 4, 2009, pages 13, XP021052326, doi:10.1186/1750-1326-4-13
See also references of EP 3578986A4
Attorney, Agent or Firm:
PARK, Young-woo (KR)
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