Title:
METHOD FOR MONOMERIZING MATRIX METALLOPROTEINASE-7 (MMP-7) AGGREGATE
Document Type and Number:
WIPO Patent Application WO/2015/178414
Kind Code:
A1
Abstract:
Provided is a method for monomerizing an MMP-7 aggregate. An MMP-7 monomerization method comprising treating an MMP-7 aggregate with a buffer solution including a low concentration of a monovalent cation chloride (such as sodium chloride, potassium chloride, etc.), or with said buffer solution not including a monovalent cation chloride. An MMP-7 preparation method incorporating said monomerization method, and a (pharmaceutical) composition containing MMP-7 dissolved in said buffer solution. When preparing a (pharmaceutical) composition containing a low concentration of MMP-7, said buffer solution is used with a sugar alcohol or sugar added.
More Like This:
Inventors:
NAKATAKE HIROSHI (JP)
HIRASHIMA MASAKI (JP)
TAKEO HIDEKI (JP)
MATSUYAMA REIKO (JP)
MORIKAWA WATARU (JP)
HIRASHIMA MASAKI (JP)
TAKEO HIDEKI (JP)
MATSUYAMA REIKO (JP)
MORIKAWA WATARU (JP)
Application Number:
PCT/JP2015/064451
Publication Date:
November 26, 2015
Filing Date:
May 20, 2015
Export Citation:
Assignee:
CHEMO SERO THERAPEUT RES INST (JP)
International Classes:
C12N9/64; A61K38/46; A61K47/02; A61K47/10; A61K47/16; A61K47/26; A61P19/00; A61P25/00; C12N9/96
Domestic Patent References:
| WO2012167271A1 | 2012-12-06 | |||
| WO2010047347A1 | 2010-04-29 |
Foreign References:
| JP2008206491A | 2008-09-11 | |||
| JP2009517086A | 2009-04-30 | |||
| JP2013518893A | 2013-05-23 | |||
| JP2005006509A | 2005-01-13 | |||
| JP2005528109A | 2005-09-22 | |||
| JPH10276774A | 1998-10-20 | |||
| JP2000344672A | 2000-12-12 |
Other References:
LOPEZ DE TURISO J ET AL.: "Expression and purification of human matrilysin produced in baculovirus-infected insect cells", J. BIOTECHNOL., vol. 46, no. 3, 1996, pages 235 - 241, XP004036792, ISSN: 0168-1656
HARO H: "Translational research of herniated discs: current status of diagnosis and treatment", J. ORTHOP. SCI., vol. 19, no. 4, July 2014 (2014-07-01), pages 515 - 520, XP055238066, ISSN: 0949-2658
OU L ET AL.: "The expression and refolding of isotopically labeled recombinant Matrilysin for NMR studies", PROTEIN EXPR. PURIF., vol. 47, no. 2, 2006, pages 367 - 373, XP024908753, ISSN: 1046-5928
KANNAN R ET AL.: "Purification of active matrix metalloproteinase catalytic domains and its use for screening of specific stromelysin-3 inhibitors", PROTEIN EXPR. PURIF., vol. 16, no. 1, 1999, pages 76 - 83, XP004441684, ISSN: 1046-5928
BROWNER MF ET AL.: "Matrilysin-inhibitor complexes: common themes among metalloproteases", BIOCHEMISTRY, vol. 34, no. 20, 1995, pages 6602 - 6610, XP000983457
BARNETT J ET AL.: "Production, purification, and characterization of human matrilysin (PUMP) from recombinant Chinese hamster ovary cells", PROTEIN EXPR. PURIF., vol. 5, no. 1, 1994, pages 27 - 36, XP024799933, ISSN: 1046-5928
KIHIRA Y ET AL.: "Production of recombinant human matrix metalloproteinase 7 (Matrilysin) with potential role in tumor invasion by refolding from Escherichia coli inclusion bodies and development of sandwich ELISA of MMP-7", UROL. ONCOL., vol. 2, no. 1, 1996, pages 20 - 26, XP055238069
ZHOU JH ET AL.: "Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma", WORLD J. GASTROENTEROL., vol. 17, no. 10, 2011, pages 1373 - 1378, XP055238070, ISSN: 1007-9327
INOUYE K ET AL.: "States of tryptophyl residues and stability of recombinant human matrix metalloproteinase 7 (matrilysin) as examined by fluorescence", J. BIOCHEM., vol. 128, no. 3, 2000, pages 363 - 369, XP055238071
SAMUKANGE V ET AL.: "Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 78, no. 1, 2014, pages 41 - 48, XP055238072, ISSN: 0916-8451
CRABBE T ET AL.: "Biochemical characterization of matrilysin. Activation conforms to the stepwise mechanisms proposed for other matrix metalloproteinases", BIOCHEMISTRY, vol. 31, no. 36, 1992, pages 8500 - 8507, XP055238073
ONEDA H ET AL.: "Refolding and recovery of recombinant human matrix metalloproteinase 7 (matrilysin) from inclusion bodies expressed by Escherichia coli", J. BIOCHEM., vol. 126, no. 5, 1999, pages 905 - 911, XP055238074, ISSN: 0021-924x
See also references of EP 3147358A4
HARO H: "Translational research of herniated discs: current status of diagnosis and treatment", J. ORTHOP. SCI., vol. 19, no. 4, July 2014 (2014-07-01), pages 515 - 520, XP055238066, ISSN: 0949-2658
OU L ET AL.: "The expression and refolding of isotopically labeled recombinant Matrilysin for NMR studies", PROTEIN EXPR. PURIF., vol. 47, no. 2, 2006, pages 367 - 373, XP024908753, ISSN: 1046-5928
KANNAN R ET AL.: "Purification of active matrix metalloproteinase catalytic domains and its use for screening of specific stromelysin-3 inhibitors", PROTEIN EXPR. PURIF., vol. 16, no. 1, 1999, pages 76 - 83, XP004441684, ISSN: 1046-5928
BROWNER MF ET AL.: "Matrilysin-inhibitor complexes: common themes among metalloproteases", BIOCHEMISTRY, vol. 34, no. 20, 1995, pages 6602 - 6610, XP000983457
BARNETT J ET AL.: "Production, purification, and characterization of human matrilysin (PUMP) from recombinant Chinese hamster ovary cells", PROTEIN EXPR. PURIF., vol. 5, no. 1, 1994, pages 27 - 36, XP024799933, ISSN: 1046-5928
KIHIRA Y ET AL.: "Production of recombinant human matrix metalloproteinase 7 (Matrilysin) with potential role in tumor invasion by refolding from Escherichia coli inclusion bodies and development of sandwich ELISA of MMP-7", UROL. ONCOL., vol. 2, no. 1, 1996, pages 20 - 26, XP055238069
ZHOU JH ET AL.: "Autoantibodies against MMP-7 as a novel diagnostic biomarker in esophageal squamous cell carcinoma", WORLD J. GASTROENTEROL., vol. 17, no. 10, 2011, pages 1373 - 1378, XP055238070, ISSN: 1007-9327
INOUYE K ET AL.: "States of tryptophyl residues and stability of recombinant human matrix metalloproteinase 7 (matrilysin) as examined by fluorescence", J. BIOCHEM., vol. 128, no. 3, 2000, pages 363 - 369, XP055238071
SAMUKANGE V ET AL.: "Effects of heparin and cholesterol sulfate on the activity and stability of human matrix metalloproteinase 7", BIOSCIENCE, BIOTECHNOLOGY, AND BIOCHEMISTRY, vol. 78, no. 1, 2014, pages 41 - 48, XP055238072, ISSN: 0916-8451
CRABBE T ET AL.: "Biochemical characterization of matrilysin. Activation conforms to the stepwise mechanisms proposed for other matrix metalloproteinases", BIOCHEMISTRY, vol. 31, no. 36, 1992, pages 8500 - 8507, XP055238073
ONEDA H ET AL.: "Refolding and recovery of recombinant human matrix metalloproteinase 7 (matrilysin) from inclusion bodies expressed by Escherichia coli", J. BIOCHEM., vol. 126, no. 5, 1999, pages 905 - 911, XP055238074, ISSN: 0021-924x
See also references of EP 3147358A4
Attorney, Agent or Firm:
SAMEJIMA, Mutsumi et al. (JP)
Mutsumi Sameshima (JP)
Mutsumi Sameshima (JP)
Download PDF: