"METHOD FOR MORPHOLOGICALLY ALTERING AND INHIBITING THE VIRAL FUNCTIONS"

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FIELD OF THE ART

The present invention relates to a method for morphologically altering and inhibiting the viral functions, with particular reference to the reproductive capability of the virus, specifically usable for studying and evaluating the viral behaviour in specific conditions.

In particular, the object of the method is the morphological alteration wherein a magnetic field at a suitable intensity industrial frequency, as will be described below, which generates a thermal effect is used.

In particular, the object of the method is the functional inhibition wherein a frequency-modulated radio wave wherein the modulating element is in ELF band is used or a radio wave coupled with a magnetic field in ELF band is used.

STATE OF THE ART

In the document by Francesca Pica et al "Effect of extremely low frequency electromagnetic fields (ELF-EMF) on Kaposi ' s sarcoma- associated herpes virus in BCBL-1 cells", preliminarily, the authors state that "the association between electromagnetic fields (ELF-EMF) and human tumours e controversial" .

This is a dated statement showing two factor orders; the authors do not know the interaction mechanism between ELF and living matter and ignore the so-called Libof f-Zhadin effect (the combination of DC and AC magnetic fields according to a certain ion cyclotron frequency: see the 4 articles in section A in Giuliani L. and Soffritti M. "Non thermal effects and mechanisms of interaction between electromagnetic fields and living matter", Eu J Of Oncology Library vol.5, Mattiolil885 S2010 Oct. Free download at www.icems.eu) , also because the effect was not fully explained before the document by Zhadin M. and Giuliani L. "Some problems in modern bioelectromagnetics , Electrom Biol Med 2006, 25 (4) :269" and still after the paper by Del Giudice E. et al "On the "unreasonable" effects of ELF magnetic fields upon a system of ions", Bioelectromagnetism 2002 October; 23 (7) :522-30. doi: 10.1002/bem.10046.

The effect, discovered in 1985 by Liboff A.R., regarding the ions, and by Zhadin MN and Novikov W regarding the zwitterions, published in Russian on Biofizika, then repeated at the University of Colorado together with Barnes F.S. and Pergola N. and published on Bioelectromagnetics 1998, 19 (l) :41-45 doi: 10.1002/ (sici) 1521- 186x (1998 ) 19 : 1<41 : : aid-bem4>3.0. co; 2-4, may be the technical effect that is assumed not to be found in the Italian research report, even if it is acknowledged by a lot of those skilled in the art anywhere in the world.

After discovering the Libof f-Zhadin effect, and the explanation thereof, the mentioned statement by Francesca Pica et al in the document mentioned as relevant in the Italian research report is no longer acceptable.

Indeed, in 2009 Novikov V.V. et al published an article showing the action of the Zhadin effect on the Ehrlich murine tumour, entitled "Effect of weak combined static and extreme-low- frequency alternating magnetic fields on tumor growth in mice inoculated with the Ehrlich ascites carcinoma" in Bioelectromagnetics 2009 Jul;30 (5) :343-51.doi: 10.1002/bem.20487.

Another article, wherein modulating the malignancy of a type of cancer is related to the Zhadin effect, is by Ledda M. et al, "Non ionising radiation as a non chemical strategy in regenerative medicine: Ca (2+)-ICR "In Vitro" effect on neuronal differentiation and tumorigenicity modulation in NT2 cells", in PLOS ONE 2013 Apr 9; 8 (4 ) : e61535. doi: 10.1371 / journal . pone .0061535.

Many other studies followed in the last ten years in addition to the authorization by FDA to the treatment of patients suffering from brain tumours by Optune, a medical device by Novocure Ltd (USA) irradiating non-thermal radio waves with a frequency comprised between 100 and 200 KHz .

A very important work in the field of clinical treatment against cancer is that by Costa F.P. et al "Treatment of advanced hepatocellular carcinoma with very low levels of amplitude- modulated electromagnetic fields" Br J Cancer 105, 640-648 (2011) . https : // doi . org/10.1038/bjc.2011.292, wherein it is stated that "A s ingle-group, open-label, phase I/II study was performed to assess the safety and effectiveness of the intrabuccal administration of very low levels of electromagnetic fields amplitude modulated at HCC-specific frequencies in 41 patients with advanced HCC and limited therapeutic options... median overall survival was 6. 7 months (95% CI 3.0-10.2) . There were three partial and one near complete responses" (Abstract) . The subministred carrier wave "frequency (27.12 MHz) was sinusoidally modulated at specific frequencies" , 194 frequency modulations every 60 ' in the ELF/ULF band (Main) .

The selection of the modulating frequencies is bypassed by scanning all the frequencies in the range above, probably because at the time the authors were not able to select single frequencies as effective as the ion cyclotron frequencies of efficient ions and zwitterions in the treatment, i.e., that suggested in the present patent application.

In conclusion, it can be stated that the above document, mentioned in the Italian research report, is not relevant nor useful in the field of application of the present invention, and that the present patent application contains a significant and surprising progress with respect to the mentioned existing literature.

The same conclusion about the article by Pica could be anticipated, starting from the article mentioned in the Italian research report by Staczek John et al "Low frequency electromagnetic field alter the replication cycle of MS2 bacterio f age" , Current microbiology 1998, 36 ( 5) : 298-301.

It is reported, the effect of the magnetic field of 60 Hz 0.5 and 2.5 microteslas on the bacteriophage MS2, concluding: "It is the simplest biological system wherein an effect induced by the electromagnetic fields has been demonstrated" . Actually, also this statement was quite ambitious: the effects induced by the electromagnetic fields have been demonstrated by W. Ross Adey already in the early 1970s (see "'Biological Effects of Radio Frequency Electromagnetic Radiation" , chapter in W. Ross Adey. Electromagnetic Interaction with Biological Systems, 1989, ISBN: 978-1-4684-8061-0, a periodical) .

Surprisingly, even in the old article by Staczek J. et al the discriminating factor of the intensity of the industrial magnetic field at 0.5 microteslas is reported, i.e., "the EMF has not altered the final phage concentration", while experiments at 2.5 microteslas "suggested that the strongest field resulted in both the obstacle to the phage replication and the increase of the phage yield" .

This is the reason why our invention, our method, requires an intensity of more than 2 microteslas in view of peeling the viruses of the pseudo-villi thereof (spike protein in the case of SARS-2) , because an intensity of 1 microtesla (as in the document by Pica) is not sufficient for morphological effects on cells and viruses .

RU 2 414 931 Cl is not relevant as the intensities of the electromagnetic fields used in the reported work are not specified. As specified by Mikhail Zhadin there is a very wide difference between the intensities required to move zwitterions (such as glutamic acid) , of the order of 10 nanoTeslas and to move alkalineS or alkaline-earthy ions, of the order of 1 microtesla. Furthermore, in altering the morphology of complex systems such as viruses, macrophages or cells, intensities of about 2-3 microteslas are required. Therefore, RU 2 414 931 Cl is not citable as a document describing the same subject being worked on. In conclusion, both the article by Staczek and that by Pica do not use the entire range 0-100 Hz, which is necessary wishing to use the Libof f-Zhadin effect, because for each ion or zwitterion there is a difference regarding the cyclotron frequency, proportional to the ratio q/m, where q is the charge and m the mass of the ion or zwitterion. On the other hand, in the present invention the industrial frequency (50 Hz) is used to achieve a thermal effect on the target to cause a specific morphological damage (peeling) not a mix of functional and morphological damages, being unexpected because the authors of the mentioned article do not seem to know the unitary theory on the basis of their electromagnetic effects.

One more time, the phenomenological knowledge of an effect - a first time on the cells of the Kaposi' s sarcoma, a second time on the bacteriophage MS2 - does not involve that there is a theory and a method for facing different viruses or bacteria, as in the object of the method of the invention.

Thus Pica and Staczek, using an industrial magnetic field, have nothing to do with the present invention based on the Liboff- Zhadin effect and on the ion cyclotron resonance, wishing to use the ELF thermal effect; the present invention, based on the industrial magnetic field when a thermal effect is required, teaches how to achieve a specific object (target peeling) and not an unexpected effect such as that in the article by Pica, i.e., "The viral progeny produced under exposure to ELF-EMF mainly consisted of defective viral particles" .

The specific object is achieved because the ion cyclotron resonance has modified the distribution of the electric charge of the virions, as taught by the case of the SARS-2 spike protein (see Kocherova A et al, "Computational modelling of protein conformational changes - Application to the opening SARS-CoV-2 spike." J Computational Physics 2021 Nov, volume 444, 110591 https : / /www . science di re ct . com/ science/article/pii/S002199912100486 1) •

This is the same effect being used in our invention.

The thermal effect of the magnetic field at an industrial frequency has been connected to the passage of electric current which would be harmful above the density threshold of 100 mA/m ^Λ 2 (ICNIRP Guidelines 1998, 2020) . However, at 2 mA/m ^Λ 2, which would result from exposing the human body to 0.1 mT (exposure limit for population in Italy and reference level in the EU) , the field would be tolerated. And so it would be, by the workers, for exposures to 6 mT, on equipotential surfaces (Directive 2013/35/EU) . The thermal effect of the low-frequency current, induced by the variable magnetic field at a low frequency, is however responsible also for other phenomena including certain morphological alterations of the cytoskeleton of the human cells, which arise when exposing the body to a number of milliteslas of the industrial frequency.

Such effect is one of the three effects intended to be included here in the method for stemming the viral proliferation in the case of viruses wherein the morphological perfection plays a decisive role for proliferation, such as coronaviruses or adenoviruses, or HIV and HIV2.

The second effect is the interference in distributing the electrostatic charges of their spike proteins. In the case of coronaviruses, the spike proteins are articulated outside in two protein complexes SI and S2, disposed facing each other, where the same sign or opposite sign electric charges are distributed on the opposed surfaces of the two complexes so as to repel or attract each other depending on the functional requirements of the infective attack. Due to this, the virus attacks the host cell by a pincer movement of the two protein complexes, which can be advantageously interfered by an electromagnetic field in radiofrequency, especially in the upper longwave radio band.

The third effect is the ion cyclotron effect which may concern the essential amino acids and enzymes, regarding the antioxidant effect, and the cytokines, in particular interleukin-6 and interleukin-10, regarding the effect inhibiting the cytokine storm and generally anti-inflammatory, and any other biomolecule involved in the infection progression.

In order to operate on the first effect, it is expected to administer to a viral sample a magnetic field at an industrial frequency or slightly more, of such an intensity to cause a thermal effect but less than that involved by the safety standards for workers; in order to operate on the second effect, it is expected to use a long wave of the radio with intensities still related to a non- thermal effect and therefore compatible with the safety standards; in order to achieve the third effect, it is expected to operate with cyclotron frequencies, i.e., generally less than 100 Hz, in the presence of the geomagnetic field detectable at our latitude, selected according to the ion resonance effect to the biomolecule to be put in resonance. Such effect is amplified when exposing the hydronium ion to the ion resonance frequency, which increases the conductivity of the intracellular water.

The last two effects may be achieved separately, as described above, or simultaneously by the exposure to a long radio wave amplitude modulated with two modulating elements at a cyclotron frequency, one accorded to the hydronium ion, the other to the biomolecule selected for treating the virus .

The ion cyclotron resonance has been identified as an interaction between electromagnetic fields at a low frequency and living matter following the reproduction in different Countries of the so-called Libof f-Zhadin effect.

In particular, an important biological interaction has been acknowledged with reference to the action of the electromagnetic fields at a very low frequency on the capillary ion and transmembrane motion.

Applying this chemical-physical effect to biology has lead to surprising results in regenerative medicine by stimulating the metabolism and differentiation and cell maturation in neuronal and pseudo-neuronal cells, normal or cancerous epithelial cells, cardiac cells and even in bone and cartilage cells.

The ion cyclotron resonance appears to play an important role also in preventing and treating degenerative diseases such as sporadic and hereditary Alzheimer, showing an ability to reduce the brain p-amyloid plaques and also an anti-bacterial action, being possible to modulate the production of biofilm by the antibiotic- resistant bacteria.

However, the known devices are still affected by defects and disadvantages which do not allow such technology to be efficiently used, despite the huge rising interest of the countless possible applications in the medical field.

The main problem of the known devices is that they are not generally constructed to use a known bioelectromagnetic interaction, but they are constructed on an empirical basis, and they are generally closed systems, with a preset list of operative conditions to be presented to the therapist or experimenter, which thus cannot freely select frequencies, wave forms or intensities to be administered, but can only select a set of preset values.

Even the most advanced devices, such as those mentioned for treating tumours, do not allow the single operating parameters to be precisely and accurately analysed and selected in order to optimize the oncostatic effect.

Furthermore, the known systems are not even configured to actually operate in an ion cyclotron resonance condition as they are calibrated, designed and controlled as a function of the module of the geomagnetic field, without considering the magnetic inclination of the latter. In order to better understand the difference, it should be considered the formula which allows to determine the frequency of the variable field, once the static field is known, for the ion cyclotron resonance : where v is the frequency of the variable field and Bo the module of the static field parallel to the variable field.

Consider the divalent calcium ion. Assuming Bo=47.8 ml - the value of the module of the geomagnetic field measured by the Observatory of Castel Telesino (TN) , on 29/12/2019 - the ion cyclotron resonance frequency of calcium will be equal to 36.37 Hz.

However, considering the magnetic inclination Io for determining the horizontal or vertical component, i.e., the one that will be coupled to the variable magnetic field to be administered, then Bo=47.8*cos (62.38° ) or Bo=47.8* sin ( 62.38 ° ) , leading to an ion cyclotron resonance frequency of calcium equal to 16.86 Hz or 42.35 Hz, respectively.

With such discordances of values of the variable magnetic field, no Libof f-Zhadin effect is determinable for no ion, thus making the ion cyclotron resonance actually inapplicable in known devices .

OBJECT OF THE INVENTION

In this context, the technical task being the basis of the present invention is to propose a method using the thermal effect of the electromagnetic fields at a low frequency (first described effect) and the consequent effect on actin or similar proteins forming pseudo-villi and the interfering or coupling effect of the long radio waves on the surface charge distributions and on the conductivity in cells and tissues (for the muscle described in the publication of WHO Environmentale Health Criteria 137, Geneva

1992) and, thirdly, the ion cyclotron resonance in an efficient and versatile manner in order to amplify and accelerate the metabolism and the consequent functional contribution of different species of biomolecules, allowing the application thereof in a wide variety of situations, among which there is in particular the scope of the biotechnological development and research in order to study the viral behaviour. To this end, it is useful that the used device, as well as being able to emit long radio waves and magnetic fields at a low frequency, with the said intensities, is also suitable for selecting the ion cyclotron resonance frequencies relative to ions and zwitterions of the inorganic and organic chemistry, including the hydronium hydrates.

The clarified technical task and the specified objects are substantially achieved by a method, comprising the technical characteristics set forth in one or more of the attached claims . According to the present invention a method for morphologically altering and inhibiting the viral functions is shown.

The method is carried out by generating at least one of: i) an electromagnetic field in the longwave radio band, for interference with the electrostatic configurations of the viral surface proteins, and at least one magnetic field in the extremely/ultra-low frequency -ELF/ULF- band: ii) an electromagnetic field at an industrial frequency or greater, up to 3 KHz and with an intensity in the order of milliteslas, so as to generate a thermal effect suitable for inducing a morphological alteration of a virus, in particular of the envelope or the pseudo-villi as far as external thereto; iii) an electromagnetic field at a frequency lower than 100 Hz, with an intensity in the order of microtesla suitable for inducing an ion cyclotron resonance which controls the movement of a specific molecule within the virus surface protein complex; iv) an electromagnetic field at a frequency lower than 30 Hz, with an intensity in the order of microtesla or however in the range of 0, 01-10 microteslas, suitable for mobilising the hydrates of the hydronium ion in the same protein complex and in the virus nucleus, increasing the conductance in modulus thereof .

In a preferred embodiment of the invention, the method is carried out by generating i) an electromagnetic field in the longwave radio band, for interference with the electrostatic configurations of the viral surface proteins, and at least three magnetic fields in the extremely/ultra-low frequency -ELF/ULF band; and ii) an electromagnetic field at an industrial frequency or greater, up to 3 KHz and with an intensity in the order of milliteslas, so as to generate a thermal effect suitable for inducing a morphological alteration of a virus, in particular of the envelope or the pseudo-villi as far as external thereto .

Thus, it proceeds to generate an operating signal (preferably controlling a biconical radio antenna and/or a plurality of coils) at a long radio wave frequency, between 30 kHz and 300 kHz, and two at a frequency in the ELF/ULF band (up to 3000 Hz) , one for increasing the water conductivity by administering the cyclotron frequency of a hydronium hydrate, the other for administering the cyclotron frequency of a variable ion or zwitterion (through the progressive scanning of the other cyclotron frequencies) but interfering with a virus surface protein, that is emitting a carrier wave modulated in amplitude having a frequency comprised between 30 kHz and 300 kHz, to alter the charge distribution of a protein -s (or spike protein) of a virus with two modulations at cyclotron frequencies in the ELF band, so as to increase the water conductivity in the experimental sample and to mobilise a specific ion or zwitterion able to mobilise a viral surface protein in addition to a signal at an industrial frequency or however in the Extremely Low Frequencies (ELF) band aimed at morphologically altering the virus, envelope and spike proteins, causing a viral reproduction to be inhibited.

Preferably the operating signal is generated at a frequency comprised between 100 kHz and 200 kHz and accompanied by two signals at a frequency lower than 100 Hz for the ion cyclotron resonance of a variable molecule interfering with the function of a virus surface protein and a hydronium hydrate, that is, consists of a carrier wave modulated in amplitude having a frequency comprised between 100 kHz and 200 kHz and a double modulation, at the frequency selected for the ion cyclotron resonance of a molecule (better ion or zwitterion) , variable as above, and for that of a hydronium hydrate, in both cases together with an operating signal at a frequency comprised between 50 and 100 Hz. In order to carry out the method, it is possible to use in particular devices which separately generate: i) the interfering long radio wave, ii) the magnetic field at an industrial frequency for the thermal effect, iii) the ion cyclotron resonance for selected ions and zwitterions and for the main polymers of the hydronium (e.g., the Zundel cation or the eigen cation) . The intensities of the different variable and electromagnetic magnetic fields used in the method remain within the exposure limits for workers .

Advantageously, the method according to the present invention allows a particularly efficient treatment to be performed with reference to morphologically altering and applicably inhibiting the viral functions even and in particular for studying and checking the behaviour of viruses in biological tissues and/or cell cultures .

The dependent claims, integrated here for reference, correspond to different embodiments of the invention. BRIEF DESCRIPTION OF THE DRAWINGS

Further characteristics and advantages of the present invention will appear clearer from the description indicative, and therefore not limiting, of a preferred, but not exclusive, embodiment of a method for the ion cyclotron resonance, as illustrated in the attached drawings wherein:

- figure 1 shows a diagram representing the main constitutive elements of a device usable for carrying out the method according to the present invention;

- figure 2 shows a picture of a coronavirus pre and post treatment of the invention, explaining the inhibiting effect of the method according to the invention.

DETAILED DESCRIPTION OF THE EMBODIMENTS

In the attached figures the reference number 1 generically denotes a device for the ion cyclotron resonance and the emission of ELF waves of intensity higher than millitesla, coupled to a biconical antenna for long waves, which will be referred to in the following part of the present disclosure simply as device 1.

Such device represents an exemplary model particularly suitable for carrying out the method according to the present invention. However, the method may be advantageously carried out also through devices for the ion cyclotron resonance having a different structure, as long as comprising a plurality of coils operable in the operating modes provided and required by the method independent claim.

Generally, it is observed that the device 1 can be specifically configured to operate in the ELF band, both for generating and using magnetic fields at an industrial frequency or greater and for generating and using ion cyclotron resonances, with frequencies belonging to the range of the extremely low frequencies .

In particular, the device 1 is configured or can be configured to generate at least one of the following electromagnetic fields : i) an electromagnetic field in the longwave radio band, for interference with the electrostatic configurations of the viral surface, and at least three magnetic fields in the extremely/ultra-low frequency -ELF/ULF- band: ii) an electromagnetic field at an industrial frequency or greater, up to 3 KHz and with an intensity in the order of milliteslas, so as to generate a thermal effect suitable for inducing a morphological alteration of a virus; iii) an electromagnetic field at a frequency lower than 100 Hz, with an intensity in the order of microtesla suitable for inducing an ion cyclotron resonance which controls the movement of a specific molecule within the virus surface protein complex; iv) an electromagnetic field at a frequency lower than 15 Hz, with an intensity in the order of microtesla, suitable for mobilising the hydrates of the hydronium ion in the same protein complex and in the virus nucleus, increasing the conductance in modulus thereof .

In a preferred embodiment of the invention, the method is carried out by generating i) an electromagnetic field in the longwave radio band, for interference with the electrostatic configurations of the viral surface proteins, and at least three magnetic fields in the extremely/ultra-low frequency -ELF/ULF band; and ii) an electromagnetic field at an industrial frequency or greater, up to 3 KHz and with an intensity in the order of milliteslas, so as to generate a thermal effect suitable for inducing a morphological alteration of a virus, in particular of the envelope or the pseudo-villi as far as external thereto .

The ion cyclotron resonance is a phenomenon related to the movement of ions in a magnetic field and of a voltage signal delivered at a suitable frequency. In distilled water, such phenomenon also relates to zwitterions (in this regard, see Zhadin M., Giuliani L. 2006. Some problems in modern bioelectromagnetics. Electrom. Biol. Med. 25 (4) : 227-43. doi : 10.1080/15368370601066195) .

Under the action of the magnetic field and of an applied voltage, the charged particles are accelerated according to a spiral movement .

The ion cyclotron resonance is not limited to inducing cell ion currents of inorganic ions but may be also applied in the health sector as it may be also applied to organic molecules and is based on a precise mechanism, by which it is possible to induce in the cells, also in vivo, ion currents, also originating from heavy ions and zwitterions, using for their formation and persistence the energy released by water molecule clusters, present in the cells, which appears to be confirmed by the different mentioned experimental biological applications, from the modulation of the cell maturation, to the modulation of the production of film by antibiotic-resistant bacteria, etc.

Generally, the method described herein is aimed at inhibiting the reproductive capability of a virus, without wishing to exclude thereby a wider applicability thereof for analysing and studying prokaryotic and eukaryotic cells.

In particular, the inhibition of the reproductive capabilities of the virus is achieved by two distinct approaches: a first approach of a morphological type and a second approach of a functional type .

As will be examined below, in accordance with the first approach, the device is operated so as to generate an operating signal "C" at such a frequency to thermally interact with the virus by morphologically altering it until inhibiting the reproductive capability thereof.

In this context the operating signal "C" determines a thermal stimulation at an industrial frequency or however ELF/ULF comprised in the range of 30-3000 Hz, preferably comprised in the range of 50-100 Hz. On the other hand, the second approach involves generating an operating signal "C" at such a frequency to generate a long or medium wave frequency radio interference thus comprised for example in the range of 30-3000 KHz) , preferably comprised between 100 and 300 KHz, which functionally alters the virus deactivating the reproductive capability thereof .

When acting as above described on the virus, the method involves, as already mentioned, acting on the host, i.e., on the potential biological tissue/cell culture the virus is in, in order to neutralize the virus through the contribution of specific molecules, controlled by the ion cyclotron resonance and aimed at containing in the host the infection and also the immune reaction leading to the cytokine storm and thereby promoting the mobility of both ions or zwitterions (pairs of ions bonded by an ionic bond) with anti-inflammatory properties and regulatory ions such as interleukins, in particular IL-6 and IL-10.

By way of example and not limitation, express reference will be made in the following description to a situation wherein the device is configured to operate so as to inhibit the in vitro viral reproduction in a sample which is subjected to one or more cycles of ion cyclotron resonance, of exposure to an industrial frequency, of exposure to long radio wave, with intensities lower than the current exposure limits for workers.

In accordance with a possible applicative scope, the device 1 comprises an exposure system 2, a signal generator 3, an impedance meter 4 and at least one gaussmeter 5.

The exposure system 2 comprises a plurality of coils 2a configured to generate an electromagnetic field within a treatment volume "T" and a support 2b for a sample, in addition to a biconical antenna configured to emit long radio waves and signal generators thereof . In particular, the coils 2a have respective inner surfaces defining the treatment volume "T" suitable for accommodating the sample (or however in more general terms the biological tissue and/or the cell culture having the viral agent to be morphologically altered or functionally deactivated) therein.

In other words, the coils 2a have a substantially annular conformation and size such that the inner diameter thereof is sufficiently large to allow a sample to be positioned therein, therefore the inner surface of the coils makes a portion of the wall of the treatment volume "T".

A biconical antenna positioned in axis with the coils allows the sample to be irradiated to the long radio waves even simultaneously with the exposure to the variable magnetic fields of the coils .

In this context, the plurality of coils 2a comprises a plurality of Helmholtz coils, preferably four Helmholtz coils.

Generally, the Helmholtz coils consist of identical circular magnetic coils which are placed symmetrically along a common axis and equidistant from each other so as to be smoothly distributed with respect to the treatment volume "T" they delimit.

Preferably, the Helmholtz coils are separated by a distance equal to the radius of the coils themselves, thereby minimizing the non- uniformity of the electromagnetic field at the centre of the coils themselves .

In particular, using the Helmholtz coils, suitably inserted in a high mu metal tube, allows the interference of the geomagnetic field to be minimized, producing a region with a magnetic field intensity much closer to zero.

Further details with regard to the specific structure and functionality of the Helmholtz coils are specified in the following article:

- Beiranvand, R. Analyzing the uniformity of the generated magnetic field by a practical one-dimensional Helmholtz coils system. Rev. Sci. Instrum. 2013, 84, 075109.

Operatively, the Helmholtz coils are disposed coaxially along a horizontally disposed main axis extending along a lying plane of the sample 2b.

In this way, the sample 2b may be inserted or insertable within the treatment volume "T" by sliding the same along the main axis.

In other words, the Helmholtz coils define a substantially cylindrical treatment volume "T" within which the sample may be disposed in a fixed or removable manner.

For example, a cage or a bed may be movable on horizontal guides which allow it to slide entering or exiting the treatment volume V ip

Preferably, in this context, the device 1 further comprises a compensation coil, also makeable in the form of a Helmholtz coil, configured for compensating the vertical component of the geomagnetic field.

In particular, the compensation coil is disposed perpendicularly with respect to the other coils of the device 1, therefore being the Helmholtz coils usually aligned with their horizontally disposed central axis, the compensation coil is disposed so as to have a vertically disposed central axis.

In use, it may be further advantageous to place the cage or bed 2b according to a north-south alignment which further allows the interference due to the horizontal component of the geomagnetic field to be eliminated.

In accordance with a further possible embodiment, the support 2b is placed on a support made of insulating material within a plurality of Merritt coils when using the device 1.

In this context, the plurality of coils 2a comprises a plurality of Merritt coils, preferably comprises three Merritt coils, or Cao coils .

Further details with regard to the specific structure and functionality of the Merritt coils are indicated in the following article :

- Merritt R, Purcell C and G Stroink (USA 1983) . Uniform magnetic field produced by three, four, and five square coils. Review of Scientific Instruments 1983 March, 54:879; Epub Nov 4th 1998; DOI :10.1063/1.1137480.

In particular, the Merritt coils are disposed coaxially along a preferably vertical main axis, that is the Merritt coils are overlapped to define a vertically developing stack of coils .

The sample may be located on the support within the treatment volume "T" ; that is within the space delimited by the Merritt or Cao coils .

In particular, the Cao coils represent a specific construction conformation of the Merritt coils, the specific structure and functionality thereof are indicated in the following article: Qinjie Cao, Donghua Pan, Ji Li, Yinxi Jin, Zhiyin Sun, Shengxin Lin, Guijie Yang and Liyi Li (CHN 2018) . Optimization of a Coil System for Generating Uniform Magnetic Fields inside a Cubic Magnetic Shield. Energies 2018, 11, 608

Generally, and regardless of the specific structural conformation of the exposure system 2, the device 1 further comprises at least one gaussmeter 4 configured to measure an intensity value of a spatial distribution of the electromagnetic field, in particular within the treatment volume "T" .

Preferably, the at least one gaussmeter 4 provides the three spatial coordinates of the magnetic field in the treatment volume and is a precision gaussmeter, that is it has a high sensitivity preferably equal to or less than 25 nanoTeslas (nT) , that is, it is able to identify and distinguish variations of the magnetic field equal to or less than 25 nT .

The signal generator 3 is instead configured to control the plurality of coils 2a.

In particular, the signal generator 3 is configured to generate an operating signal "C" having a frequency comprised between 30 kHz and 300 kHz (preferably comprised between 100 and 200 kHz) , as required for carrying out the method, and a sinusoidal or triangular or square or sawtooth waveform by which the electromagnetic field is modulable by controlling the plurality of coils 2a.

The signal generator 3 may be also configured to generate an operating signal "C" comprising a carrier wave modulated in amplitude and having a frequency comprised between 30 kHz and 300 kHz (preferably comprised between 100 and 200 kHz) , as required for carrying out the method, by which the electromagnetic field is modulable by controlling the plurality of coils 2a.

In particular, a greater biological effectiveness in the exposure to a carrier wave modulated in amplitude at a low or very low frequency rather than directly to the waves at a low frequency has been observed, as discussed for example in the following publications :

- Bawin SM, Adey WR, Sabbot IM (USA 1978) . Ionic factors in release of 45Ca2+ from chicken cerebral tissue by electromagnetic fields. PNAS 1978 Dec; 75 (12 ) : 6314-8 ;

- Lyle DB, Schechter P, Adey WR, Lundak RL . Suppression of T- lymphocyte cytotoxicity following exposure to sinusoidally amplitude-modulated fields. Bioelectromagnetics . 1983 ; 4 (3 ) : 281-92 ;

- Blackman CF, Benane SG, House DE, Joines WT (USA 1985) . Effects of ELF (1-120 Hz) and modulated (50 Hz) RF fields on the efflux of calcium ions from brain tissue in vitro. Bioelectromagnetics. 1985; 6 (1) : 1-11;

- Vignati M, Giuliani L. radiofrequency near high voltage power lines . Environ Health Persp 105 (S6) :1535.

The device 1 may further comprise a plurality of sensors configured to detect a respective plurality of biological parameters and for controlling the microclimate and the sample cultures .

In particular, the microclimatic control signal could detect an excessive stress condition of the sample when using the device 1 and thus determine an immediate interruption of the procedure (for example blocking the generation of the operating signal "C") or generate an error/warning signal of an acoustical and/or optical type so as to warn an operator about the occurrence of a situation critical for the experiment .

Furthermore, as already mentioned in order to reduce the interference of the geomagnetic field, the device 1 may comprise at least one protective casing 6 disposed around the plurality of coils 2a and cooperating therewith to define the treatment volume

In other words, the protective casing 6 may define a physical wall for the treatment volume "T" which covers in particular the wall portions of the treatment volume "T" left free from the coils 2a. Such protective casing 6 is configured to shield the treatment volume "T" from the geomagnetic field, thus preventing that the latter can disturb each variable magnetic field defined according to the formula within the treatment volume "T" .

Advantageously, using the above-illustrated devices 1 allows, in the cell cultures, in at least one of the above-discussed aspects, to partially destructure the cell cytoskeleton, due to the electromagnetic action at 50 Hz of the actin which causes the collapse thereof inside the cytoskeleton in cells and viruses provided with pseudo-villi.

In the case of picoRNA infections, preferably coronavirus, even more preferably SARS 2 infections, the infection mechanism has been identified in the spike protein (S) , consisting of two domains (S1-S2) , and the binding function thereof would be in the domain SI of the same spike protein while in the domain S2 there would be the molecule which lyses the membrane of the host cell and allows the virus to penetrate.

In the case of coronavirus, the infection mechanism has been identified in the spike protein (S) , consisting of two domains (S1-S2) , as in SARS1-CoVid09 and the binding function thereof would be in the domain SI of the same spike protein while in the domain S2 there would be the molecule which lyses the membrane of the host cell and allows the virus to penetrate.

In SARS2-Covid19 the domain SI would be activated by the furin enzyme, as by the receptor ACE2 of the host cell, a receptor similar to the receptor of SARS1-CoVid09, ACE.

In particular, the spike protein rests, outside the virus envelope, on an actin-like filament which could collapse by the exposure to the frequency of 50 Hz, or more within 100 Hz, with an amplitude in the order of milliteslas, as the actin filaments collapse in certain eukaryotic cells.

In this case, the spike proteins of the coronavirus would collapse all together finishing in the virus envelope (the physical interactions of sufficient amplitude affect all the target population, at the same instant) ; and they could no longer bind the furin or ACE2 of any host cell.

Generally, the effects set forth above have a duration in the order of hour and however persisting for the entire time of the exposure, which could be continuous, since the exposure to the industrial magnetic field at a few milliteslas is comparable with the exposure limit for workers valid in Italy and in other European Countries and the potential use of higher exposure values would however fall, for the purposes of the present invention, within the higher LA (6 ml at the industrial frequency) , for the respect of which the electrical ground would be sufficient.

The treatment which can be carried out by the present device 1 is aimed at destructuring the pseudo-actinic filaments of the viruses and thus would be efficient to block the reproductive (and thus infective) capability of the viruses.

In a clinical applicative context, the potential weakness due to a smaller mobility of lymphocytes B could be modulated with a specific exposure on the target organs, also through an exhibitor- probe, in the cases of intubations, which does not involve if not marginally the circulating blood.

On the other hand, is the exposure promotes the release of angiotensin, this could, contribute to soften the inflammatory effect of the cytokine storm, caused by the virus, and would result synergic with heparin treatment, the high-dose treatment thereof has been authorized, on an experimental basis, by AIFA.

The treatment of CoViD19, as of SARS, in severe and critical phase could be assisted by the exposure to ELF/SLF, at an ion cyclotron resonance frequency, related to interleukin 6 and interleukin 10, in order to limit the cytokine storm caused by the coronavirus.

The same ELF/SLF treatment may be adopted with other coronaviruses, with influenza viruses, with syncytial pneumonia virus and generally with picoRNA viruses and in virus diseases having binding proteins on extruded filaments of the envelope .

In particular, the two parts S1 and S2 of the Spike protein S are mutually movable and a potential difference up to 0.5 V is established therebetween, in the binding step.

The frequencies between 100 and 200 kHz (referred to as preferential for the device described here) are able to alter the charge distribution of the protein -s (which would rise from the opposition of homologous charges within the cell membrane during the mitosis) , thus preventing the cell reproduction, which is, as known, much more frequent in tumour cells than in healthy cells. Similarly, the indicated frequencies would alter the charge distribution between the subset S1-S2 of the Spike protein preventing the operativity of the two subsets, especially of S2 in the lysis of the membrane of the host cell for pouring the viral material therein.

The method object of the present invention is particularly suitable for being applied for morphologically altering and inhibiting the functionality of the CoVid-19 virus and is explicitly carried out by providing a device 1 having one or more characteristics among those indicated above, in particular provided with a plurality of coils controllable according to the operative requirements of the method. In detail, the method is carried out by activating the device 1 at least one electromagnetic field in accordance with the following specifications : i) an electromagnetic field in the longwave radio band, for interference with the electrostatic configurations of the viral surface, and at least three magnetic fields in the extremely/ultra-low frequency -ELF/ULF- band: ii) an electromagnetic field at an industrial frequency or greater, up to 3 KHz and with an intensity in the order of milliteslas, so as to generate a thermal effect suitable for inducing a morphological alteration of a virus; iii) an electromagnetic field at a frequency lower than 100 Hz, with an intensity in the order of microtesla suitable for inducing an ion cyclotron resonance which controls the movement of a specific molecule within the virus surface protein complex; iv) an electromagnetic field at a frequency lower than 30 Hz, with an intensity in the order of microtesla, suitable for mobilising the hydrates of the hydronium ion in the same protein complex and in the virus nucleus, as in the cell substrate, increasing the conductance in modulus thereof .

In more detail, the above-described deliver of the magnetic fields is controlled by generating an operating signal "C" at a frequency comprised between 30 kHz and 300 kHz (preferably comprised between 100 and 200 kHz) , preferably having a sinusoidal or triangular or square or sawtooth waveform, or comprising a carrier wave modulated in amplitude having a frequency comprised between 30 kHz and 300 kHz (preferably comprised between 100 and 200 kHz) , with double modulation in ELF band, so as to alter a charge distribution of a protein -s of a virus, thereby causing the inhibition of viral reproduction.

Synergically the operating signal can be generated at a frequency comprised between 30 Hz and 3000 Hz, preferably comprised between 50 Hz and 100 Hz, so as to morphologically alter the virus, thereby causing the inhibition of viral reproduction.

Both the exposures may occur at intensities lower than those of the exposure limits for workers, thereby not needing particular expedients in an operative environment, except for the electrical ground .

Preferably, the viral infection is a coronavirus infection and the step to be controlled causes the deactivation of the Spike S1-S2 protein by inhibiting a cell membrane lysis capability by said Spike S1-S2 protein.

Figure 2 shows a picture confirming such inhibiting effect.

In particular, fig. 2A shows a pre-exposure virus, fig. 2B shows the virus after being exposed to 50 Hz, 1 mT for 64 hours.

As can be seen from the figures, in fig. 2A structures similar to microvilli are detectable, while in fig. 2B such structures disappear and a greater amplitude of the membrane is observed, probably due to the introf lection of the structures similar to microvilli .

The method also involves detecting a plurality of parameters for controlling the microclimate of the sample and for evaluating the static magnetic field in the treatment volume "T" .

In particular, the shielding is carried out by applying to the device 1 a protective casing (6) , preferably a protective casing 6 consisting of a high mu metal material or a metal glass, preferably having the structure/composition described above.

Advantageously, furthermore, the operation of the plurality of coils may be controlled using an operating signal comprising a doubly carrier wave modulated in amplitude preferably with ion cyclotron frequencies (lower than 100 Hz) .

In this way, it is possible to extend the interference spectrum of the signal emitted by the device (1) by disposing the electrostatic configurations at the interface of the complexes SI and S2 of coronaviruses and faces or of other surface proteins of other viruses, having similar conformation and function.

The experimented possibility to operate both with a radio emission coupled to the emission of two magnetic fields at a cyclotron frequency and with a doubly amplitude modulated radio emission with modulating elements at the desired cyclotron frequencies rests on the fact that in eukaryotic cells many proteins operate as diodes and separate the carrier frequency from the modulating frequency allowing them to act in a biologically independent manner .

Separately and in addition to using a radio frequency, alone or amplitude modulated, respectively coupled or modulated with frequencies for the ion cyclotron resonance, for the above- described purposes of mobilising an electrified molecule for interference with a viral surface protein in addition to increasing the conductivity in the cultures and tissues, the method involves using an industrial frequency or however between 20 and 3000 Hz, preferably between 50 and 100 Hz, promoting the destructuring of the envelope and the actin-like pseudo-villi of the viruses .

In conclusion, the present method concerns the use of long radio waves modulated at an ELF frequency or coupled to two ripples in ELF band at an ion cyclotron resonance frequency for the inhibition of viral functions and using ELF magnetic fields, preferably at industrial frequencies at such an intensity to cause a thermal effect able to destructure the viral envelope and the pseudo-villi structure, the spike proteins in the case of coronaviruses, by inhibiting in both cases the reproductive capability of the viruses .

Preferably the use of such electromagnetic waves is performed by using the above-described device 1.

The invention further provides using electromagnetic waves modulated at an ion cyclotron resonance frequency for treating viral infections, preferably picoRNA infections, preferably coronavirus, even more preferably SARS 2 infections.

The method, of the invention causes a deactivation of the Spike Sl- S2 protein by inhibiting a cell membrane lysis capability by the Spike S1-S2 protein.

In an embodiment, the invention provides a method for treating and/or inhibiting viral infections, preferably picoRNA infections, comprising the steps of :

- providing the device 1 for the ion cyclotron resonance configured or can be configured to generate electromagnetic fields in an extremely low frequency - ELF - mode;

- activating the device 1 generating an operating signal C:

- at a frequency comprised between 30 kHz and 300 kHz or comprising a carrier wave with a frequency comprised between 30 kHz and 300 kHz and amplitude modulated in an ELF band with an amplitude greater than 1 mT, so as to alter a charge distribution of a spike protein of a virus, thereby causing the inhibition of viral reproduction, or

- at a frequency comprised between 30 Hz and 3000 Hz, preferably comprised between 50 Hz and 100 Hz, so as to morphologically alter the virus, thereby causing the inhibition of viral reproduction;

- at a frequency lower than 300 Hz, preferably comprised between 1 and 100 Hz, in order to generate ion cyclotron frequencies so as to mobilise ions within cells and biological tissues, in particular for antioxidant, anti-inflammatory and/or probiotic purposes.

Preferably, the viral infection is a coronavirus infection, preferably SARS 2 infection, and the step of activating the device causes the deactivation of the Spike S1-S2 protein by inhibiting a cell membrane lysis capability by said Spike S1-S2 protein. Preferably, the step of providing a device for the ion cyclotron resonance is carried out by providing a device comprising:

- an exposure system 2 comprising a plurality of coils 2a having respective inner surfaces defining a treatment volume T suitable for accommodating at least partially a user and configured to generate an electromagnetic field in the treatment volume T;

- an impedance meter 4 configured to measure a specific impedance value of a user when exposed to the electromagnetic field within the treatment volume T and to generate a specific impedance signal I representative of the specific impedance value;

- at least one gaussmeter 5 configured to measure an intensity value of a spatial distribution of the electromagnetic field within the treatment volume T and to generate a reference signal R representative of the intensity value;

- a signal generator 3 configured to control the plurality of coils 2a by modulating one or more characteristics of the electromagnetic field simultaneously as a function of the specific impedance signal I and the reference signal R.

Preferably, the step of providing a device for the ion cyclotron resonance is carried out by providing a device 1 that comprises a waveguide or a TEM cell configured to contain a sample to be analysed and insertable into an industrial incubator.

Preferably, the method comprises the steps of :

- detecting a plurality of physiological parameters;

- generating a physiological signal representative of at least one of said physiological parameters; said plurality of physiological parameters preferably comprising at least one of : a blood pressure value, a blood oxygen value, a blood glucose value .

Preferably, the activation of the device 1 is carried out by modulating one or more characteristics of the operating signal C also as a function of the physiological signal.

Preferably, the operating signal C has a sinusoidal or triangular or square or sawtooth waveform.

Preferably, a step of shielding a treatment volume T of the device 1 from a geomagnetic field is provided. Preferably, the shielding step is carried out by applying to the device 1 a protective casing 6.

Preferably, the operating signal C comprises a carrier wave modulated in amplitude at an ion cyclotron frequency so as to promote cell repairing actions by ion cyclotron resonance.

Preferably, the operating signal C is generated at a frequency comprised between 100 kHz and 200 kHz and is modulated in amplitude with two modulating waves comprising a first modulating wave at a cyclotron frequency of a hydronium ion hydrate and a second modulating wave at a cyclotron frequency of a biomolecule, preferably an antioxidant or anti-inflammatory biomolecule.