Title:
METHOD FOR OBTAINING ENZYME MUTANT HAVING HIGH EXPRESSION, HIGH VIABILITY AND HIGH STABILITY
Document Type and Number:
WIPO Patent Application WO/2019/169689
Kind Code:
A1
Abstract:
Provided are an alkaline pectinase mutant and a preparation method therefor. The enzyme mutant is obtained from a library of functional polypeptides constructed on the basis of SAPs.
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Inventors:
LIU SONG (CN)
CHEN JIAN (CN)
DU GUOCHENG (CN)
ZHAO WEIXIN (CN)
CHEN JIAN (CN)
DU GUOCHENG (CN)
ZHAO WEIXIN (CN)
Application Number:
PCT/CN2018/082642
Publication Date:
September 12, 2019
Filing Date:
April 11, 2018
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N15/70; C07K19/00; C12N1/21; C12N9/02; C12N9/82; C12N9/88; C12N15/62; C12N15/65; C12R1/19
Other References:
DATABASE Protein 6 February 2018 (2018-02-06), SUNEJA, G.: "pectate lyase [Bacillus sp. Ru63]", XP055637567, retrieved from NCBI Database accession no. POX35456. 1
ZHAO, WEIXIN ET AL.: "Analysis of the Factors Influencing the Expression of Enzymes fused with Self-Assembling Amphipathic Peptides", FOOD AND FERMENTATION INDUSTRIES, vol. 43, no. 12, 25 December 2017 (2017-12-25)
WANG, MINGXING ET AL.: "Fusions of Amphipathic Peptide to the Alkaline Polygalacturonate Lyase from Bacillus sp. WSHB04-02 Improves the Production", JOURNAL OF FOOD SCIECE AND BIOTECHNOLOGY, vol. 35, no. 5, 31 May 2016 (2016-05-31)
ZHAO, WEIXIN ET AL.: "Analysis of the Factors Influencing the Expression of Enzymes fused with Self-Assembling Amphipathic Peptides", FOOD AND FERMENTATION INDUSTRIES, vol. 43, no. 12, 25 December 2017 (2017-12-25)
WANG, MINGXING ET AL.: "Fusions of Amphipathic Peptide to the Alkaline Polygalacturonate Lyase from Bacillus sp. WSHB04-02 Improves the Production", JOURNAL OF FOOD SCIECE AND BIOTECHNOLOGY, vol. 35, no. 5, 31 May 2016 (2016-05-31)
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO.,LTD. (CN)
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