Title:
METHOD FOR OVEREXPRESSING PROTEIN IN YEAST
Document Type and Number:
WIPO Patent Application WO/2018/110641
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for stably causing high expression of a protein in yeast, and relates specifically to a protein production method including a step for culturing a yeast transformant in which a 2 µm plasmid is reconstructed in a yeast not maintaining the 2 µm plasmid, the reconstructed 2 µm plasmid containing a gene that codes for a desired protein.
Inventors:
AKADA RINJI (JP)
HOSHIDA HISASHI (JP)
MISUMI YUKIE (JP)
MATSUDA HITOSHI (JP)
HOSHIDA HISASHI (JP)
MISUMI YUKIE (JP)
MATSUDA HITOSHI (JP)
Application Number:
PCT/JP2017/044879
Publication Date:
June 21, 2018
Filing Date:
December 14, 2017
Export Citation:
Assignee:
UNIV YAMAGUCHI (JP)
JXTG NIPPON OIL & ENERGY CORP (JP)
JXTG NIPPON OIL & ENERGY CORP (JP)
International Classes:
C12P21/00; C12N1/19; C12N15/09
Domestic Patent References:
| WO2008139591A1 | 2008-11-20 | |||
| WO2015156369A1 | 2015-10-15 |
Foreign References:
| US20050084972A1 | 2005-04-21 | |||
| JPH0841098A | 1996-02-13 | |||
| JPH01503275A | 1989-11-09 | |||
| JP2017036223A | 2017-02-16 |
Other References:
FUTCHER, A. B.: "The 2 µm circle plasmid of Saccharomyces cerevisiae", YEAST, vol. 4, 1988, pages 27 - 40, XP055493050
CHINO, A. ET AL.: "Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe", PLOS ONE, vol. 5, no. 3, 2010, pages e9652, XP055095577
MISUMI, SACHIE ET AL.: "YHp Plasmid: Protein Overproduction using 2 µrn Plasmid Reconstruction on Brewer's Yeast", 69TH SBJ ANNUAL MEETING, PROCEEDINGS, 8 August 2017 (2017-08-08), pages 202
CHINO, A. ET AL.: "Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe", PLOS ONE, vol. 5, no. 3, 2010, pages e9652, XP055095577
MISUMI, SACHIE ET AL.: "YHp Plasmid: Protein Overproduction using 2 µrn Plasmid Reconstruction on Brewer's Yeast", 69TH SBJ ANNUAL MEETING, PROCEEDINGS, 8 August 2017 (2017-08-08), pages 202
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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