Title:
METHOD FOR THE PREPARATION OF CALCIUM-CHELATING PEPTIDE USING GINGKO NUT SHELLS
Document Type and Number:
WIPO Patent Application WO/2017/215312
Kind Code:
A1
Abstract:
A method using gingko nut shells for the preparation of calcium-chelating peptide comprises the following steps: (1) placing the gingko nut shells in an aqueous solution of NaOH, agitating same and, after settling, removing the supernatant liquid, adjusting the pH value of said supernatant liquid to between 2 and 3, then again settling, the precipitate being thus a crude protein of the gingko nut shells; (2) preparing the crude protein of the gingko nut shells as an aqueous solution, adding thereto a proteinase to cause a hydrolysis reaction, then finally inactivating the enzyme and obtaining a hydrolysed solution; (3) precipitating the hydrolysed solution by centrifuging, extracting the supernatant and vacuum-lyophilizing same to prepare a peptide; (4) preparing a solution of calcium salts and the peptide of step (3), conducting a chelation reaction; when the reaction is complete, centrifuging and precipitating to obtain a supernatant, adding ethanol to same and leaving to still, then centrifuging and precipitating and drying the precipitate to obtain the calcium-chelating peptide. The obtained calcium-chelating peptide can be used as an antioxidant, and shows a high removal rate for DPPH free radicals.
Inventors:
WANG HANG (CN)
WANG DAPING (CN)
WANG DAPING (CN)
Application Number:
PCT/CN2017/078553
Publication Date:
December 21, 2017
Filing Date:
March 29, 2017
Export Citation:
Assignee:
RUGAO FUDA ENGINEERING AND TECH RESEARCH INSTITUTE CO LTD (CN)
International Classes:
C12P21/06; C07K1/14; C07K1/30
Foreign References:
| CN106119324A | 2016-11-16 | |||
| CN102337318A | 2012-02-01 | |||
| CN104232719A | 2014-12-24 | |||
| CN103609831A | 2014-03-05 | |||
| CN105567775A | 2016-05-11 | |||
| CN103880919A | 2014-06-25 |
Attorney, Agent or Firm:
PACIFIC CHINA PATENT AGENT CO., LTD. (CN)
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