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Title:
METHOD FOR PREPARATION OF HUMAN PLURIPOTENT STEM CELL-DERIVED FUNCTIONAL CARDIOMYOCYTE THROUGH CELL DIFFERENTIATION AND MATURATION AND HUMAN PLURIPOTENT STEM CELL-DERIVED FUNCTIONAL CARDIOMYOCYTE PREPARED BY SAME METHOD
Document Type and Number:
WIPO Patent Application WO/2019/160193
Kind Code:
A1
Abstract:
The present invention relates to a method for preparation of human pluripotent stem cell-derived functional cardiomyocytes through cell differentiation and maturation, the method comprising: a culturing step of seeding and culturing human pluripotent stem cells (hPSC) in a Matrigel-coated culture medium; a mesoderm induction step of inducing the differentiation of the human pluripotent stem cells that have undergone the culturing step to the mesoderm by culturing the human pluripotent stem cells (hPSC) that have undergone the culturing step, in a mesodermal induction medium containing insulin-free B27 and a wnt signaling activator; a post-mesodermal induction differentiation step of seeding and culturing the cells that have undergone the mesoderm induction step, in a differentiation medium containing insulin-free B27; a myocardial differentiation inducing step of inducing myocardial differentiation of the cells that have undergone (the post-mesodermal induction differentiation step by culturing the cells that have undergone the post-mesodermal induction differentiation step, in a myocardial differentiation inducing medium containing insulin-free B27 and a wnt signaling regulator; a myocardial differentiation step of seeding and culturing the cells that have undergone the myocardial differentiation inducing step, in a differentiation medium containing insulin-free B27; and a cardiomyocyte maturation step of maturing the differentiated cardiomyocytes that have undergone the myocardial differentiation step into functional cardiomyocytes by culturing in a myocardial maturation medium containing vitamin A-free B27, interleukin-15, and nerve growth factor (NGF).

Inventors:
WOO DONGHUN (KR)
JO GUNSIK (KR)
HEO JINJU (KR)
KIM SEONGMIN (KR)
YOON JEONGHYUN (KR)
Application Number:
KR2018/004195
Publication Date:
August 22, 2019
Filing Date:
April 10, 2018
Export Citation:
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Assignee:
NEXEL CO LTD (02580, KR)
International Classes:
C12N5/077
Foreign References:
KR20170036485A2017-04-03
KR20170141995A2017-12-27
KR20090115142A2009-11-04
KR20090044247A2009-05-07
KR20090090586A2009-08-26
Attorney, Agent or Firm:
LEE, Jaeman (414ho 26-gil, 111 Digital-roGuro-gu, Seoul, 08390, KR)
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