Title:
METHOD FOR PREPARING 1,3-DIOLEOYL-2-PALMITOYLGLYCEROL IN A SUBCRITICAL SYSTEM
Document Type and Number:
WIPO Patent Application WO/2017/084241
Kind Code:
A1
Abstract:
Provided is a method for preparing 1,3-dioleoyl-2-palmitoylglycerol in a subcritical system, comprising the following steps: (1) preparation of raw materials: selecting, as a raw material A, lard having a cholesterol content of less than 0.05% by mass, or selecting, as raw material A, restructured palm oil having an sn-2 palmitic acid content of not less than 60% by mass; and, selecting as a raw material B, at least one of oleic acid and an oleic acid ester; and (2) subcritical enzyme-catalyzed transesterification: in a subcritical system, enabling raw materials A and B to undergo selective enzymatic transesterification in a reaction solvent under the catalysis of lipase to obtain 1,3-dioleoyl-2-palmitoylglycerol. With a rapid reaction rate, high selectivity, minimal enzyme usage, and no solvent residue, this method is readily adapted to large-scale continuous production.
Inventors:
HUANG FENGHONG (CN)
ZHENG MINGMING (CN)
WAN CHUYUN (CN)
TANG HU (CN)
GUO PINGMEI (CN)
SHI JIE (CN)
DENG QIANCHUN (CN)
ZHENG MINGMING (CN)
WAN CHUYUN (CN)
TANG HU (CN)
GUO PINGMEI (CN)
SHI JIE (CN)
DENG QIANCHUN (CN)
Application Number:
PCT/CN2016/079818
Publication Date:
May 26, 2017
Filing Date:
April 21, 2016
Export Citation:
Assignee:
OIL CROPS RES INST CHINESE ACAD OF AGRICULTURAL SECIENCES (CN)
International Classes:
C12P7/64
Domestic Patent References:
| WO2007029018A1 | 2007-03-15 |
Foreign References:
| CN105219813A | 2016-01-06 | |||
| CN101157945A | 2008-04-09 |
Other References:
LI, XINQIAN ET AL: "Enzymatic Acidolysis of Lard to Produce 1-10 1,3-Dioleoyl-2-Palmitoylglycerol by Candida", CHINESE JOURNAL OF BIOPROCESS ENGINEERING,, vol. 11, no. 3, 31 May 2013 (2013-05-31), pages 99 - 125, ISSN: 1672-3678
WAN, JIANCHUN ET AL: "Advances in Enzymatic Synthesis of 1,3-Dioleoyl-2-Palmitoyl 1-10 Glycerol", CHINA DAIRY INDUSTRY, vol. 39, no. 7, 31 July 2011 (2011-07-31)
WAN, JIANCHUN ET AL: "Advances in Enzymatic Synthesis of 1,3-Dioleoyl-2-Palmitoyl 1-10 Glycerol", CHINA DAIRY INDUSTRY, vol. 39, no. 7, 31 July 2011 (2011-07-31)
Attorney, Agent or Firm:
CHINA USIPF INTELLECTUAL PROPERTY LAW OFFICE (CN)
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