Title:
METHOD FOR PREPARING ADHERENT-TYPE CELLS ACCLIMATED TO SUSPENSION CULTURE, METHOD FOR INDUCING EPITHELIAL-MESENCHYMAL TRANSITION IN ADHERENT-TYPE EPITHELIAL CELLS, AND USE FOR METHODS
Document Type and Number:
WIPO Patent Application WO/2017/104821
Kind Code:
A1
Abstract:
The present invention is: a method for preparing adherent-type cells acclimated to suspension culture, the method having a step for culturing adherent-type cells in a state of contact with a molded article of a polymer containing an alicyclic structure; a method for producing a nucleic acid or a protein coded by a foreign gene, by culturing of the adherent-type cells acclimated to suspension culture by the aforementioned method; an agent for promoting acclimation of adherent-type cells to suspension culture, comprising a molded article of a polymer containing an alicyclic structure; the use of a molded article of a polymer containing an alicyclic structure, for causing acclimation of adherent-type cells to suspension culture in a liquid medium; a container for acclimation of adherent-type cells to suspension culture, at least a bottom surface of the container comprising a molded article of a polymer containing an alicyclic structure; a method for inducing epithelial-mesenchymal transition, the method having a step for culturing adherent-type epithelial cells in a state of contact with a molded article of a polymer containing an alicyclic structure; and a method for producing a nucleic acid or a protein coded by a foreign gene, by culturing of adherent-type cells in which epithelial-mesenchymal transition is induced by the aforementioned method.
Inventors:
HIRANO TAKAAKI (JP)
MIMURA YUMIKO (JP)
MIMURA YUMIKO (JP)
Application Number:
PCT/JP2016/087606
Publication Date:
June 22, 2017
Filing Date:
December 16, 2016
Export Citation:
Assignee:
ZEON CORP (JP)
International Classes:
C12N5/07; C08F232/00; C08G61/08; C12M3/00; C12N5/16
Domestic Patent References:
| WO2014138455A1 | 2014-09-12 | |||
| WO2014038025A1 | 2014-03-13 | |||
| WO2012081629A1 | 2012-06-21 | |||
| WO2015199119A1 | 2015-12-30 | |||
| WO2015199117A1 | 2015-12-30 | |||
| WO2006101925A2 | 2006-09-28 |
Foreign References:
| JPH029388A | 1990-01-12 | |||
| JP2005073509A | 2005-03-24 |
Other References:
RAZA A. ET AL.: "The influence of matrix properties on growth and morphogenesis of human pancreatic ductal epithelial cells in 3D", BIOMATERIALS, vol. 34, 2013, pages 5117 - 5127, XP028593862, DOI: doi:10.1016/j.biomaterials.2013.03.086
KI C.S. ET AL.: "Thiol-ene hydrogels as desmoplasia-mimetic matrices for modeling pancreatic cancer cell growth, invasion, and drug resistance", BIOMATERIALS, vol. 35, 2014, pages 9668 - 9677, XP029055289, DOI: doi:10.1016/j.biomaterials.2014.08.014
TOKUZO ARAO ET AL.: "EMT Kenkyu", MOLECULAR TARGETED THERAPY FOR CANCER, vol. 9, no. 1, 2011, pages 61 - 64
MASSAGUE J, CELL, vol. 134, July 2008 (2008-07-01), pages 215 - 230
See also references of EP 3392334A4
KI C.S. ET AL.: "Thiol-ene hydrogels as desmoplasia-mimetic matrices for modeling pancreatic cancer cell growth, invasion, and drug resistance", BIOMATERIALS, vol. 35, 2014, pages 9668 - 9677, XP029055289, DOI: doi:10.1016/j.biomaterials.2014.08.014
TOKUZO ARAO ET AL.: "EMT Kenkyu", MOLECULAR TARGETED THERAPY FOR CANCER, vol. 9, no. 1, 2011, pages 61 - 64
MASSAGUE J, CELL, vol. 134, July 2008 (2008-07-01), pages 215 - 230
See also references of EP 3392334A4
Attorney, Agent or Firm:
OHISHI Haruhito (JP)
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