Title:
METHOD FOR PREPARING ALPHA-KETOPIMELIC ACID BY C1-ELONGATION
Document Type and Number:
WIPO Patent Application WO/2012/031910
Kind Code:
A3
Abstract:
The invention relates to a method for preparing alpha-ketopimelic acid, comprising converting alpha-ketoglutaric acid into alpha-ketoadipic acid and converting alpha-ketoadipic acid into alpha-ketopimelic acid, wherein at least one of these conversions is carried out using a heterologous biocatalyst catalysing at least one of these conversions, wherein the heterologous biocatalyst comprises a. an NifV enzyme or another Aks enzyme having homo(n)citrate activity or a homologue thereof having homo(n)citrate activity; b. an AksD enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, c. an AksE enzyme having homon-aconitase activity or a homologue thereof having homon-aconitase activity, d. an AksF enzyme having homon- isocitrate dehydrogenase or a homologue thereof having homon-aconitase activity.
More Like This:
Inventors:
TREFZER AXEL CHRISTOPH (NL)
TURK STEFANUS CORNELIS HENDRIKUS JOZEF (NL)
TURK STEFANUS CORNELIS HENDRIKUS JOZEF (NL)
Application Number:
PCT/EP2011/064718
Publication Date:
June 07, 2012
Filing Date:
August 26, 2011
Export Citation:
Assignee:
DSM IP ASSETS BV (NL)
TREFZER AXEL CHRISTOPH (NL)
TURK STEFANUS CORNELIS HENDRIKUS JOZEF (NL)
TREFZER AXEL CHRISTOPH (NL)
TURK STEFANUS CORNELIS HENDRIKUS JOZEF (NL)
International Classes:
C12P7/50; C12N9/02; C12N9/10; C12N9/88; C12P13/00; C12P7/44; C12P13/02; C12P17/10
Domestic Patent References:
| WO2010068944A2 | 2010-06-17 | |||
| WO2009113855A2 | 2009-09-17 | |||
| WO2008000632A1 | 2008-01-03 | |||
| WO2010104390A2 | 2010-09-16 | |||
| WO2011031147A1 | 2011-03-17 |
Other References:
HOWELL DAVID M ET AL: "alpha-Keto acid chain elongation reactions involved in the biosynthesis of coenzyme B (7-mercaptoheptanoyl threonine phosphate) in methanogenic archaea", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 37, no. 28, 14 July 1998 (1998-07-14), pages 10108 - 10117, XP002503245, ISSN: 0006-2960, DOI: 10.1021/BI980662P
DREVLAND RANDY M ET AL: "Methanogen Homoaconitase Catalyzes Both Hydrolyase Reactions in Coenzyme B Biosynthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 283, no. 43, 24 October 2008 (2008-10-24), pages 28888 - 28896, XP002570860, ISSN: 0021-9258, [retrieved on 20080902], DOI: 10.1074/JBC.M802159200
GRAHAM DAVID E ET AL: "Elucidation of methanogenic coenzyme biosyntheses: from spectroscopy to genomics", NATURAL PRODUCT REPORTS, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 19, no. 2, 1 April 2002 (2002-04-01), pages 133 - 147, XP002570861, ISSN: 0265-0568, DOI: 10.1039/B103714P
DATABASE EMBL [online] 30 June 2007 (2007-06-30), "Methanococcus aeolicus Nankai-3, complete genome.", XP002639544, retrieved from EBI accession no. EMBL:CP000743 Database accession no. CP000743
DREVLAND RANDY M ET AL: "Methanogen Homoaconitase Catalyzes Both Hydrolyase Reactions in Coenzyme B Biosynthesis", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 283, no. 43, 24 October 2008 (2008-10-24), pages 28888 - 28896, XP002570860, ISSN: 0021-9258, [retrieved on 20080902], DOI: 10.1074/JBC.M802159200
GRAHAM DAVID E ET AL: "Elucidation of methanogenic coenzyme biosyntheses: from spectroscopy to genomics", NATURAL PRODUCT REPORTS, ROYAL SOCIETY OF CHEMISTRY, GB, vol. 19, no. 2, 1 April 2002 (2002-04-01), pages 133 - 147, XP002570861, ISSN: 0265-0568, DOI: 10.1039/B103714P
DATABASE EMBL [online] 30 June 2007 (2007-06-30), "Methanococcus aeolicus Nankai-3, complete genome.", XP002639544, retrieved from EBI accession no. EMBL:CP000743 Database accession no. CP000743
Attorney, Agent or Firm:
BREEPOEL, Peter, Maria (AA Echt, NL)
Download PDF: