Title:
A METHOD FOR PREPARING ANTIGENS OF FOOT-AND-MOUTH DISEASE VIRUS
Document Type and Number:
WIPO Patent Application WO/2008/116368
Kind Code:
A1
Abstract:
A method for expressing antigens of foot-and-mouth disease virus in insect in
vivo by using recombinant baculovirus is disclosed, which comprises: cloning
different genes combination of foot-and-mouth disease virus into baculovirus
carrying vector to construct transfer vector, transfecting baculovirus with
the transfer vector to proceed DNA recombination, and thus to get recombinant
baculovirus, infecting insect host with the recombinant baculovirus, culturing
the infected insect host to express the antigens of foot-and-mouth disease virus,
and then collecting and purifying the expressed antigens. The preferred genes,
baculovirus carrying vector, baculovirus and insect host are selected from
P1-2A3C, ORF and VP1 genes of foot-and-mouth disease virus as shown in SEQ ID NO
1, 3 or 5, pVL1393, Bombyx mori Bm-NPV-ZJ8 and larvae or pupa of Bombyx mori, respectively.
Inventors:
LIU JIXING (CN)
ZHANG ZHIFANG (CN)
LI ZHIYONG (CN)
YI YONGZHU (CN)
YIN XIANGPING (CN)
BAI YINMEI (CN)
LI YINV (CN)
LI BAOYU (CN)
LI XUERUI (CN)
YANG BIN (CN)
LAN XI (CN)
SHEN GUIFANG (CN)
ZHANG ZHIFANG (CN)
LI ZHIYONG (CN)
YI YONGZHU (CN)
YIN XIANGPING (CN)
BAI YINMEI (CN)
LI YINV (CN)
LI BAOYU (CN)
LI XUERUI (CN)
YANG BIN (CN)
LAN XI (CN)
SHEN GUIFANG (CN)
Application Number:
PCT/CN2008/000126
Publication Date:
October 02, 2008
Filing Date:
January 17, 2008
Export Citation:
Assignee:
LANZHOU VETERINARY RES INST CH (CN)
BIOTECHNOLOGY RES INST CHINESE (CN)
LIU JIXING (CN)
ZHANG ZHIFANG (CN)
LI ZHIYONG (CN)
YI YONGZHU (CN)
YIN XIANGPING (CN)
BAI YINMEI (CN)
LI YINV (CN)
LI BAOYU (CN)
LI XUERUI (CN)
YANG BIN (CN)
LAN XI (CN)
SHEN GUIFANG (CN)
BIOTECHNOLOGY RES INST CHINESE (CN)
LIU JIXING (CN)
ZHANG ZHIFANG (CN)
LI ZHIYONG (CN)
YI YONGZHU (CN)
YIN XIANGPING (CN)
BAI YINMEI (CN)
LI YINV (CN)
LI BAOYU (CN)
LI XUERUI (CN)
YANG BIN (CN)
LAN XI (CN)
SHEN GUIFANG (CN)
International Classes:
C12N15/42; A61K39/135; A61P31/12; C07K14/09; C12N7/01; C12N15/866; C12R1/03
Foreign References:
| KR20060057397A | 2006-05-26 | |||
| CN1730101A | 2006-02-08 | |||
| CN101121938A | 2008-02-13 |
Other References:
LI G. ET AL.: "Eukaryotic expression of the VP1 gene of foot-and-mouth disease virus type O and characterization of expressed fusion protein", CHINES JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY, vol. 36, no. 3, 2006, pages 183 - 188
ZHANG X. ET AL.: "Cloning of whole ORF coding genes of FMDV and construction of recombinant adenoviral pAdTrack-CMV", CHINESE JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY, vol. 36, no. 2, 2006, pages 93 - 97
ZHAO Y. ET AL.: "Expression of ORF_5 Gene of Porcine Reproductive and Respiratory Syndrome Virus B_(13) Strain in a Baculovirus Expression System", JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 8, no. 3, 2000, pages 211 - 215
LIU Y. ET AL.: "Progress of studies and application on baculovirus expression vector system", ENTOMOLOGCIAL KNOWLEDGE, vol. 43, no. 1, 2006, pages 1 - 5
LI Z. ET AL.: "Immune response in cattle incoulated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae", CHINESE SCIENCE BULLETIN, vol. 52, no. 20, 31 October 2007 (2007-10-31), pages 2805 - 2810
ZHANG X. ET AL.: "Cloning of whole ORF coding genes of FMDV and construction of recombinant adenoviral pAdTrack-CMV", CHINESE JOURNAL OF VETERINARY SCIENCE AND TECHNOLOGY, vol. 36, no. 2, 2006, pages 93 - 97
ZHAO Y. ET AL.: "Expression of ORF_5 Gene of Porcine Reproductive and Respiratory Syndrome Virus B_(13) Strain in a Baculovirus Expression System", JOURNAL OF AGRICULTURAL BIOTECHNOLOGY, vol. 8, no. 3, 2000, pages 211 - 215
LIU Y. ET AL.: "Progress of studies and application on baculovirus expression vector system", ENTOMOLOGCIAL KNOWLEDGE, vol. 43, no. 1, 2006, pages 1 - 5
LI Z. ET AL.: "Immune response in cattle incoulated with the recombinant complete polyprotein of foot-and-mouth disease virus from Bombyx mori larvae", CHINESE SCIENCE BULLETIN, vol. 52, no. 20, 31 October 2007 (2007-10-31), pages 2805 - 2810
Attorney, Agent or Firm:
KELONG INTERNATIONAL PROPERTY AGENT LTD. et al. (Suite 13-3 Bldg. A,Horizon International Tower,No.6 Zhichun Roa, Haidian District Beijing 8, CN)
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