Title:
METHOD FOR PREPARING BETA CAROTENE CRYSTALS
Document Type and Number:
WIPO Patent Application WO/2018/028402
Kind Code:
A1
Abstract:
A method for preparing beta carotene crystals, comprising the following steps: (1) inoculating and fermenting a microbial strain, and (2) performing solid-liquid separation of the fermentation broth to obtain wet bacteria; (3) drying the wet bacteria to obtain dry bacteria; (4) using an organic solvent to perform extraction on the dry bacteria; (5) performing solid-liquid separation to obtain an extract solution; (6) evaporating and crystallising the extract solution to obtain crystal mother liquor; (7) performing solid-liquid separation of the crystal mother liquor to obtain a crude wet crystal; (8) washing the crude wet crystal in organic solvent, and vacuum drying to obtain the beta carotene crystals. An antioxidant is added in steps (3), (4), and (6). Antioxidant is added in some or all of the steps of drying, solid-liquid separation, and evaporating and crystallising, reducing the loss of beta carotene in the extraction process and improving the beta carotene yield; the purity and quality of the obtained beta carotene crystals is high.
Inventors:
LI XIANGYU (CN)
WANG ZHIMING (CN)
LU SHUHUAN (CN)
TIAN YONG (CN)
ZHOU QIANG (CN)
YI DEWEI (CN)
WANG ZHIMING (CN)
LU SHUHUAN (CN)
TIAN YONG (CN)
ZHOU QIANG (CN)
YI DEWEI (CN)
Application Number:
PCT/CN2017/093522
Publication Date:
February 15, 2018
Filing Date:
July 19, 2017
Export Citation:
Assignee:
CABIO BIOTECH WUHAN CO LTD (CN)
International Classes:
C07C403/24; C12P23/00; C12R1/645; C12R1/89
Domestic Patent References:
| WO2011145113A2 | 2011-11-24 |
Foreign References:
| CN106278975A | 2017-01-04 | |||
| CN106222226A | 2016-12-14 | |||
| CN106278976A | 2017-01-04 | |||
| CN106278977A | 2017-01-04 | |||
| CN106278978A | 2017-01-04 | |||
| CN101870668A | 2010-10-27 | |||
| CN104531538A | 2015-04-22 | |||
| CN104557648A | 2015-04-29 | |||
| CN102787158A | 2012-11-21 |
Other References:
DAI, DEHUI ET AL.: "Preparation Processing Study of Thalli Feed Richen beta-Carotene", FEED INDUSTRY, vol. 28, no. 12, 31 December 2007 (2007-12-31), pages 27 - 30
SHI, BIN ET AL.: "Extraction of beta-Carotene from Dunaliella Salina", FINE CHEMICALS, vol. 11, no. 5, 31 December 1994 (1994-12-31), pages 17 - 19
SHI, BIN ET AL.: "Extraction of beta-Carotene from Dunaliella Salina", FINE CHEMICALS, vol. 11, no. 5, 31 December 1994 (1994-12-31), pages 17 - 19
Attorney, Agent or Firm:
CN-KNOWHOW INTELLECTUAL PROPERTY AGENT LIMITED (CN)
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