Title:
METHOD FOR PREPARING BILE ACID COMPOUND VIA ENZYMATIC CATALYSIS
Document Type and Number:
WIPO Patent Application WO/2019/025029
Kind Code:
A3
Abstract:
The present invention relates to the provision of an enzymatic method for the preparation of 1 β, 3α,7α,12α-tetrahydroxy bile acid (Formula I). The enzymatic method of the present invention is advantageous over conventional synthetic preparations, providing the selective insertion of a hydroxyl group without the need of employing protective groups or other synthetic maneuvers.
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Inventors:
KOFTIS THEOCHARIS (GR)
NEOKOSMIDIS EFSTRATIOS (GR)
TERZIDIS MICHAEL (GR)
ALEXANDRAKI ELLI (GR)
GIOTI EFTHYMIA (GR)
TRAKOSSAS SAKELLARIOS (GR)
ANDREOU THANOS (GR)
VASTARDI ELLI (GR)
DRIMAROPOULOU GARYFALLIA (GR)
NEOKOSMIDIS EFSTRATIOS (GR)
TERZIDIS MICHAEL (GR)
ALEXANDRAKI ELLI (GR)
GIOTI EFTHYMIA (GR)
TRAKOSSAS SAKELLARIOS (GR)
ANDREOU THANOS (GR)
VASTARDI ELLI (GR)
DRIMAROPOULOU GARYFALLIA (GR)
Application Number:
PCT/EP2018/025208
Publication Date:
March 14, 2019
Filing Date:
August 01, 2018
Export Citation:
Assignee:
VIO CHEMICALS AG (CH)
International Classes:
C12P33/06
Other References:
K CARLSTROM ET AL: "Microbial synthesis of 1 beta-and 15 beta-hydroxylated bile acids", JOURNAL OF LIPID RESEARCH, vol. 22, 1981, pages 1225 - 1234, XP055545143
M. A. HAYES ET AL: "CYP3A Specifically Catalyzes 1[beta]-Hydroxylation of Deoxycholic Acid: Characterization and Enzymatic Synthesis of a Potential Novel Urinary Biomarker for CYP3A Activity", DRUG METABOLISM AND DISPOSITION, vol. 44, no. 9, 11 July 2016 (2016-07-11), pages 1480 - 1489, XP055545337, DOI: 10.1124/dmd.116.070805
BODIN K ET AL: "Novel pathways of bile acid metabolism involving CYP3A4", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - MOLECULAR AND CELL BIOLOGY OF LIPIDS, ELSEVIER, AMSTERDAM, NL, vol. 1687, no. 1-3, 21 February 2005 (2005-02-21), pages 84 - 93, XP027705804, ISSN: 1388-1981, [retrieved on 20050221]
MASHIKO TOHMA ET AL: "Synthesis of the 1[beta]-Hydroxylated Bile Acids, Unusual Bile Acids in Human Biological Fluids", CHEMICAL AND PHARMACEUTICAL BULLETIN, 1986, pages 2890 - 2899, XP055545305, DOI: 10.1248/cpb.34.2890
M. A. HAYES ET AL: "CYP3A Specifically Catalyzes 1[beta]-Hydroxylation of Deoxycholic Acid: Characterization and Enzymatic Synthesis of a Potential Novel Urinary Biomarker for CYP3A Activity", DRUG METABOLISM AND DISPOSITION, vol. 44, no. 9, 11 July 2016 (2016-07-11), pages 1480 - 1489, XP055545337, DOI: 10.1124/dmd.116.070805
BODIN K ET AL: "Novel pathways of bile acid metabolism involving CYP3A4", BIOCHIMICA ET BIOPHYSICA ACTA (BBA) - MOLECULAR AND CELL BIOLOGY OF LIPIDS, ELSEVIER, AMSTERDAM, NL, vol. 1687, no. 1-3, 21 February 2005 (2005-02-21), pages 84 - 93, XP027705804, ISSN: 1388-1981, [retrieved on 20050221]
MASHIKO TOHMA ET AL: "Synthesis of the 1[beta]-Hydroxylated Bile Acids, Unusual Bile Acids in Human Biological Fluids", CHEMICAL AND PHARMACEUTICAL BULLETIN, 1986, pages 2890 - 2899, XP055545305, DOI: 10.1248/cpb.34.2890
Attorney, Agent or Firm:
AIVALIOTIS, Apostolos (GR)
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