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Title:
METHOD FOR PREPARING CHONDROCYTES
Document Type and Number:
WIPO Patent Application WO/2014/148592
Kind Code:
A1
Abstract:
Provided is a method for preparing chondrocytes that makes it possible to solve problems involved in conventional methods and to construct cartilage tissue. A method for preparing chondrocytes including the co-culture of chondrogenic cells with vascular cells. A composition for cartilage regenerative medicine including chondrocytes prepared by this method. A method for screening agents effective as pharmaceuticals using chondrocytes prepared by this method, cartilage tissue formed from these chondrocytes and/or cells derived from this cartilage tissue. A method for preparing a chondrocyte-producing substrate using chondrocytes prepared by this method, cartilage tissue formed from these chondrocytes and/or cells derived from this cartilage tissue. A method for cartilage regeneration including the transplantation of chondrocytes prepared by this method into a living body and the formation of cartilage tissue.

Inventors:
TANIGUCHI HIDEKI (JP)
TAKEBE TAKANORI (JP)
KOBAYASHI SHINJI (JP)
Application Number:
PCT/JP2014/057673
Publication Date:
September 25, 2014
Filing Date:
March 20, 2014
Export Citation:
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Assignee:
PUBLIC UNIV CORP YOKOHAMA CITY (JP)
International Classes:
C12N5/077; A61K35/12; A61K35/32; A61L27/00; A61P19/08; A61P43/00; G01N33/15
Domestic Patent References:
WO2002012451A12002-02-14
WO2008091013A12008-07-31
Foreign References:
JP2012031127A2012-02-16
JP4748222B22011-08-17
JP2012000262A2012-01-05
JP2011078710A2011-04-21
JP2009226221A2009-10-08
JP2009106214A2009-05-21
Other References:
BITTNER K. ET AL.: "Role of the subchondral vascular system in endochondral ossification: endothelial cells specifically derepress late differentiation in resting chondrocytes in vitro", EXP. CELL RES., vol. 238, no. 2, pages 491 - 497, XP055283036
SAYED K.E ET AL.: "Stimulated Chondrogenesis via Chondrocytes Co-culturing", J. BIOCHIP TISSUE CHIP., vol. S2, 2012, pages 1 - 11, XP055283037
HENDRIKS J. ET AL.: "Co-culture in cartilage tissue engineering", J. TISSUE ENG. REGEN. MED., vol. 1, no. 3, June 2007 (2007-06-01), pages 170 - 178, XP055283040
KOBAYASHI ET AL., PROC. NATL. ACAD. SCI. USA, vol. 108, 2011, pages 14479 - 14484
S FANG ET AL., PLOS BIOLOGY, vol. 10, no. 10, 2012, pages E1001407
HJ. JOO ET AL., BLOOD, vol. 118, no. 8, 25 December 2010 (2010-12-25), pages 2094 - 104
CHANG, S.C.; TOBIAS, G.; ROY, A.K.; VACANTI, C.A.; BONASSAR, L.J.: "Tissue engineering of autologous cartilage for craniofacial reconstruction by injection molding", PLAST RECONSTR SURG, vol. 112, 2003, pages 793 - 799
BEAHM, E.K.; WALTON, R.L.: "Auricular reconstruction for microtia: part 1. Anatomy, embryology, and clinical evaluation", PLAST RECONSTR SURG, vol. 109, 2002, pages 2473 - 2482
FIRMIN, F.; SANGER, C.; O'TOOLE: "G. Ear reconstruction following severe complications of otoplasty", J PLAST RECONSTR AESTHET SURG, 2008
KLINE, R.M., JR.; WOLFE, S.A.: "Complications associated with the harvesting of cranial bone grafts", PLAST RECONSTR SURG, vol. 95, 1995, pages 5 - 13,14-20
LAURIE, S.W.; KABAN, L.B.; MULLIKEN, J.B.; MURRAY, J.E.: "Donor-site morbidity after harvesting rib and iliac bone", PLAST RECONSTR SURG, vol. 73, 1984, pages 933 - 938
SKOUTERIS, C.A.; SOTEREANOS, G.C.: "Donor site morbidity following harvesting of autogenous rib grafts", J ORAL MAXILLOFAC SURG, vol. 47, 1989, pages 808 - 812, XP027055056
WHITAKER, L.A. ET AL.: "Combined report of problems and complications in 793 craniofacial operations", PLAST RECONSTR SURG, vol. 64, 1979, pages 198 - 203
EPPLEY, B.L.; DADVAND, B.: "Injectable soft-tissue fillers: clinical overview", PLAST RECONSTR SURG 118, 2006, pages 98E - 106E
MATTON, G.; ANSEEUW, A.; DE KEYSER, F.: "The history of injectable biomaterials and the biology of collagen", AESTHETIC PLAST SURG, vol. 9, 1985, pages 133 - 140
NAGATA, S.: "Modification of the stages in total reconstruction of the auricle: Part I. Grafting the three-dimensional costal cartilage framework for lobule-type microtia", PLAST RECONSTR SURG, vol. 93, 1994, pages 221 - 230
MAAS, C.S.; MONHIAN, N.; SHAH, S.B.: "Implants in rhinoplasty", FACIAL PLAST SURG, vol. 13, 1997, pages 279 - 290
MATARASSO, A.; ELIAS, A.C.; ELIAS, R.L.: "Labial incompetence: a marker for progressive bone resorption in silastic chin augmentation", PLAST RECONSTR SURG, vol. 98, 1996, pages 1007 - 1014,1015
ZENG, Y.; WU, W.; YU, H.; YANG, J.; CHEN, G.: "Silicone implants in augmentation rhinoplasty", AESTHETIC PLAST SURG, vol. 26, 2002, pages 85 - 88
BERRY, L.; GRANT, M.E.; MCCLURE, J.; ROONEY, P: "Bone-marrow-derived chondrogenesis in vitro", J CELL SCI, vol. 101, 1992, pages 333 - 342
MA, H.L.; HUNG, S.C.; LIN, S.Y.; CHEN, Y.L.; LO, W.H.: "Chondrogenesis of human mesenchymal stem cells encapsulated in alginate beads", J BIOMED MATER RES A, vol. 64, 2003, pages 273 - 281, XP002410518, DOI: doi:10.1002/jbm.a.10370
TERADA, S.; FUCHS, J.R.; YOSHIMOTO, H.; FAUZA, D.O.; VACANTI, J.P.: "In vitro cartilage regeneration from proliferated adult elastic chondrocytes", ANN PLAST SURG, vol. 55, 2005, pages 196 - 201
GOESSLER, U. R. ET AL.: "Tissue engineering in head and neck reconstructive surgery: what type of tissue do we need?", EUR ARCH OTORHINOLARYNGOL, vol. 264, 2007, pages 1343 - 1356, XP019541797, DOI: doi:10.1007/s00405-007-0369-y
CAPLAN, A. I.: "Review: mesenchymal stem cells: cell-based reconstructive therapy in orthopedics", TISSUE ENG, vol. 11, 2005, pages 1198 - 1211
SHIEH, S.J.; TERADA, S.; VACANTI, J.P: "Tissue engineering auricular reconstruction: in vitro and in vivo studies", BIOMATERIALS, vol. 25, 2004, pages 1545 - 1557, XP004481687, DOI: doi:10.1016/S0142-9612(03)00501-5
TOGO, T. ET AL.: "Identification of cartilage progenitor cells in the adult ear perichondrium: utilization for cartilage reconstruction", LAB INVEST, vol. 86, 2006, pages 445 - 457, XP003024423, DOI: doi:10.1038/labinvest.3700409
DICKHUT, A. ET AL.: "Calcification or dedifferentiation: requirement to lock mesenchymal stem cells in a desired differentiation stage", J CELL PHYSIOL, vol. 219, 2009, pages 219 - 226
AFIZAH, H.; YANG, Z.; HUI, J.H.; OUYANG, H.W.; LEE, E.H.: "A comparison between the chondrogenic potential of human bone marrow stem cells (BMSCs) and adipose-derived stem cells (ADSCs) taken from the same donors", TISSUE ENG, vol. 13, 2007, pages 659 - 666, XP009133120
KOGA, H. ET AL.: "Comparison of mesenchymal tissues-derived stem cells for in vivo chondrogenesis: suitable conditions for cell therapy of cartilage defects in rabbit", CELL TISSUE RES, vol. 333, 2008, pages 207 - 215, XP019631219
SAKAGUCHI, Y.; SEKIYA, 1.; YAGISHITA, K.; MUNETA, T.: "Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source", ARTHRITIS RHEUM, vol. 52, 2005, pages 2521 - 2529, XP002580878, DOI: doi:10.1002/ART.21212
KOBAYASHI, S. ET AL.: "Presence of cartilage stem/progenitor cells in adult mice auricular perichondrium", PLOS ONE, vol. 6, 2011, pages E26393
KOBAYASHI, S. ET AL.: "Reconstruction of human elastic cartilage by a CD44+ CD90+ stem cell in the ear perichondrium", PROC NATL ACAD SCI U S A, vol. 108, 2011, pages 14479 - 14484, XP055012051, DOI: doi:10.1073/pnas.1109767108
GOSPODAROWICZ D; WESEMAN J; MORAN J.: "Presence in brain of a mitogenic agent promoting proliferation of myoblasts in low density culture", NATURE, vol. 256, 1975, pages 216 - 219
KLAGSBRUN M; LANGER R; LEVENSON R; SMITH S; LILLEHEI C: "The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage", EXP CELL RES, vol. 105, 1977, pages 99 - 108, XP024854784, DOI: doi:10.1016/0014-4827(77)90155-0
SHIANG R; THOMPSON LM; ZHU YZ; CHURCH DM; FIELDER TJ; BOCIAN M; WINOKUR ST; WASMUTH JJ: "Mutations in the transmembrane domain of FGFR3 cause the most common genetic form of dwarfism, achondroplasia", CELL, vol. 29, 1994, pages 335 - 342, XP024245673, DOI: doi:10.1016/0092-8674(94)90302-6
DENG C; WYNSHAW-BORIS A; ZHOU F; KUO A; LEDER P: "Fibroblast growth factor receptor 3 is a negative regulator of bone growth", CELL, vol. 22, 1996, pages 911 - 921
SERRA R; JOHNSON M; FILVAROFF EH; LABORDE J; SHEEHAN DM; DERYNCK R; MOSES HL: "Expression of a truncated, kinase-defective TGF-beta type II receptor in mouse skeletal tissue promotes terminal chondrocyte differentiation and osteoarthritis", J CELL BIOL, vol. 20, 1997, pages 541 - 552
FURUMATSU, T. ET AL.: "Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment", J. BIOL. CHEM, vol. 280, 2005, pages 8343 - 8350
GARCIA-RAMIREZ, M.; TORAN, N.; ANDALUZ, P.; CARRASCOSA, A.; AUDI, L.: "Vascular endothelial growth factor is expressed in human fetal growth cartilage", J BONE MINER RES, vol. 15, 2000, pages 534 - 540
GERBER, H. P. ET AL.: "VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation", NATURE MEDICINE, vol. 5, 1999, pages 623 - 628, XP002909369, DOI: doi:10.1038/9467
FURUKAWA, T.; EYRE, D. R.; KOIDE, S.; GLIMCHER, M. J.: "Biochemical studies on repair cartilage resurfacing experimental defects in the rabbit knee", J BONE JOINT SURG AM, vol. 62, 1980, pages 79 - 89
L. DANISOVIC; P. LESNY; V. HAVLAS; P. TEYSSLER; Z. SYROVA; M. KOPANI; G. FUJERIKOVA; T. TRC; E. SYKOVA; P. JENDELOVA: "Chondrogenic differentiation of human bone marrow and adipose tissue-derived mesenchymal stem cells", J. APPL. BIOMED, vol. 5, 2007, pages 139 - 150
HAVLAS V; KOS P; JENDELOVA P; LESNY P; TRC T; SYKOVA E: "Comparison of chondrogenic differentiation of adipose tissue-derived mesenchymal stem cells with cultured chondrocytes and bone marrow mesenchymal stem cells", ACTA CHIR. ORTHOP. TRAUMATOL. CECH, vol. 78, 2011, pages 138 - 144
K.H. PARK; K. NA.: "Effect of growth factors on chondrogenic differentiation of rabbit mesenchymal cells embedded in injectable hydrogels", J. BIOSCI. BIOENG, vol. 106, 2008, pages 74 - 79, XP023612884, DOI: doi:10.1263/jbb.106.74
YUAN F. JAIN RK ET AL.: "Vascular permeability and microcirculation of gliomas and mammary carcinomas transplanted in rat and mouse cranial windows", CANCER RES, vol. 54, 1994, pages 4564 - 8
KOIKE, N. ET AL.: "Tissue engineering: creation of long-lasting blood vessels", NATURE, vol. 428, 2004, pages 138 - 139
TAKEBE, T. ET AL.: "Generation of functional human vascular network", TRANSPLANT PROC, vol. 44, 2012, pages 1130 - 1133, XP028482436, DOI: doi:10.1016/j.transproceed.2012.03.039
HORVATIC, I. ET AL.: "Prognostic significance of glomerular and tubulointerstitial morphometry in idiopathic membranous nephropathy", PATHOL RES PRACT, vol. 208, 2012, pages 662 - 667
ARUFFO, A.; STAMENKOVIC, I.; MELNICK, M.; UNDERHILL, C. B.; SEED, B.: "CD44 is the principal cell surface receptor for hyaluronate", CELL, vol. 61, 1990, pages 1303 - 1313, XP024246260, DOI: doi:10.1016/0092-8674(90)90694-A
WANG, Y.; KIM, U. J.; BLASIOLI, D. J.; KIM, H. J.; KAPLAN, D. L.: "In vitro cartilage tissue engineering with 3D porous aqueous-derived silk scaffolds and mesenchymal stem cells", BIOMATERIALS, vol. 26, 2005, pages 7082 - 7094, XP025280275, DOI: doi:10.1016/j.biomaterials.2005.05.022
NEWMANAP: "Articular cartilage repair", AM J SPORTS MED, vol. 26, 1998, pages 309 - 324
HOLLANDER, A. P.; DICKINSON, S. C.; KAFIENAH, W.: "Stem cells and cartilage development: complexities of a simple tissue", STEM CELLS, vol. 28, 2010, pages 1992 - 1996
LANGER, R.; VACANTI, J. P, TISSUE ENGINEERING. SCIENCE, vol. 260, 1993, pages 920 - 926
KHADEMHOSSEINI, A.; VACANTI, J. P; LANGER, R.: "Progress in tissue engineering", SCI AM, vol. 300, 2009, pages 64 - 71
DING, B. S. ET AL.: "Inductive angiocrine signals from sinusoidal endothelium are required for liver regeneration", NATURE, vol. 468, 2010, pages 310 - 315, XP055174403, DOI: doi:10.1038/nature09493
DING, B. S. ET AL.: "Endothelial-derived angiocrine signals induce and sustain regenerative lung alveolarization", CELL, vol. 147, 2011, pages 539 - 553, XP028330380, DOI: doi:10.1016/j.cell.2011.10.003
LAMMERT, E.; CLEAVER, O.; MELTON, D.: "Role of endothelial cells in early pancreas and liver development", MECH DEV, vol. 120, 2003, pages 59 - 64
MATSUMOTO, K.; YOSHITOMI, H.; ROSSANT, J.; ZARET, K. S.: "Liver organogenesis promoted by endothelial cells prior to vascular function", SCIENCE, vol. 294, 2001, pages 559 - 563, XP055177629, DOI: doi:10.1126/science.1063889
TAKEBE, T. ET AL.: "Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor", TRANSPLANT PROC44, 2012, pages 1158 - 1161, XP028482444, DOI: doi:10.1016/j.transproceed.2012.03.038
See also references of EP 2977448A4
Attorney, Agent or Firm:
MAYAMA, Setsuko et al. (JP)
Setsuko Mayama (JP)
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