Title:
METHOD FOR PREPARING FECAL SAMPLE, FECAL SAMPLE PREPARATION SOLUTION, AND FECES COLLECTION KIT
Document Type and Number:
WIPO Patent Application WO/2010/024251
Kind Code:
A1
Abstract:
Disclosed is a method for preparing a fecal sample which can stably store a nucleic acid contained in feces without the need of any complicated procedure. Also disclosed is a fecal sample preparation solution to be used in the method. Further disclosed is a feces collection kit. Still further disclosed is a method for collecting a nucleic acid from feces by using a fecal sample prepared by the above-mentioned method and analyzing the nucleic acid. Feces collected is mixed with a fecal sample preparation solution containing, as an active ingredient, a water-soluble organic solvent containing an organic acid, thereby preparing a fecal sample which can store a nucleic acid contained therein satisfactorily.
Inventors:
TANIGAMI YASUO (JP)
NAGAOKA TOMONORI (JP)
NAGAOKA TOMONORI (JP)
Application Number:
PCT/JP2009/064789
Publication Date:
March 04, 2010
Filing Date:
August 25, 2009
Export Citation:
Assignee:
OLYMPUS CORP (JP)
TANIGAMI YASUO (JP)
NAGAOKA TOMONORI (JP)
TANIGAMI YASUO (JP)
NAGAOKA TOMONORI (JP)
International Classes:
C12N15/09; C12Q1/68
Domestic Patent References:
| WO2009066695A1 | 2009-05-28 |
Foreign References:
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| DE19928820A1 | 2000-12-21 | |||
| JPS5823796A | 1983-02-12 | |||
| JP2002511585A | 2002-04-16 | |||
| DE10021390A1 | 2001-11-15 | |||
| JP2008217019A | 2008-09-18 | |||
| JPH0511982A | 1993-01-22 | |||
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| JP2003153688A | 2003-05-27 | |||
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| JP2009064789A | 2009-03-26 |
Other References:
OLSON, J. ET AL.: "DNA stabilization is critical for maximizing performance of fecal DNA-based colorectal cancer tests", DIAGN. MOL. PATHOL., vol. 14, no. 3, 2005, pages 183 - 191, XP009150256
ITZKOWITZ, S.H. ET AL.: "Improved fecal DNA test for colorectal cancer screening", CLIN. GASTRO. HEPATOL., vol. 5, 2007, pages 111 - 117, XP005920787
LEBBAD, M. ET AL.: "PCR differentiation of Entamoeba histolytica and Entamoeba dispar from patients with amoeba infection initially diagnosed by microscopy", SCAND. J. INFECT. DIS., vol. 37, 2005, pages 680 - 685, XP008146340
VELINOV, M. ET AL.: "PCR-based methylation testing for Prader-Willi or Angelman syndromes using archived fixed-cell suspensions", GENETIC TESTING, vol. 5, no. 2, 2001, pages 153 - 155, XP008146335
MIETHING, F. ET AL.: "Effect of fixation to the degradation of nuclear and mitochondrial DNA in different tissues", J. HISTOCHEM. CYTOCHEM., vol. 54, no. 3, 2006, pages 371 - 374, XP008146309
See also references of EP 2338989A4
LIND ET AL.: "Molecular Cancer", vol. 3, 2004, article "A CpG island hypermethylation profile of primary colorectal carcinomas and colon cancer cell lines"
NILSSON ET AL., JOURNAL OF CLINICAL MICROBIOLOGY, vol. 42, no. 8, 2004, pages 3781 - 8
ITZKOWITZ, S.H. ET AL.: "Improved fecal DNA test for colorectal cancer screening", CLIN. GASTRO. HEPATOL., vol. 5, 2007, pages 111 - 117, XP005920787
LEBBAD, M. ET AL.: "PCR differentiation of Entamoeba histolytica and Entamoeba dispar from patients with amoeba infection initially diagnosed by microscopy", SCAND. J. INFECT. DIS., vol. 37, 2005, pages 680 - 685, XP008146340
VELINOV, M. ET AL.: "PCR-based methylation testing for Prader-Willi or Angelman syndromes using archived fixed-cell suspensions", GENETIC TESTING, vol. 5, no. 2, 2001, pages 153 - 155, XP008146335
MIETHING, F. ET AL.: "Effect of fixation to the degradation of nuclear and mitochondrial DNA in different tissues", J. HISTOCHEM. CYTOCHEM., vol. 54, no. 3, 2006, pages 371 - 374, XP008146309
See also references of EP 2338989A4
LIND ET AL.: "Molecular Cancer", vol. 3, 2004, article "A CpG island hypermethylation profile of primary colorectal carcinomas and colon cancer cell lines"
NILSSON ET AL., JOURNAL OF CLINICAL MICROBIOLOGY, vol. 42, no. 8, 2004, pages 3781 - 8
Attorney, Agent or Firm:
TANAI Sumio et al. (JP)
Sumio Tanai (JP)
Sumio Tanai (JP)
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