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Title:
METHOD FOR PREPARING FLAVORED FERMENTED SOYBEAN MEAL BY STEP FERMENTATION WITH MULTIPLE STRAINS
Document Type and Number:
WIPO Patent Application WO/2020/133194
Kind Code:
A1
Abstract:
Disclosed is a method for preparing a flavored fermented soybean meal by step fermentation with multiple strains, comprising the following steps: 1) aerobic fermentation with Aspergillus oryzae of adding soybean meal into water, inoculating Aspergillus oryzae in an amount of 10% to 100% on a dry solids basis, and fermenting for 12-20h; 2) fermentation with Saccharomyces cerevisiae of inoculating, after aerobic fermentation with Aspergillus oryzae is complete, a broth of Saccharomyces cerevisiae in an amount of 10% to 100% by weight on a dry solids basis, then placing layers of the material into an airtight fermentation device with the surface layer being closed for oxygen-proof operation, and fermenting for 18-48h, a viable cell count at the end of aerobic fermentation with Saccharomyces cerevisiae exceeding 10^10 cfu/g; and 3) fermentation with a lactic acid bacterium of inoculating, after fermentation with Saccharomyces cerevisiae is complete, a broth of Streptococcus thermophilus in an amount of 10% to 100% by weight on a dry solids basis, and fermenting for 18-80h until the fermentation temperature reaches 50ºC. The method solves the problems associated with existing soybean meal fermentation in which different microbial strains do not exhibit separate or excellent function, resulting in poor taste and flavor of the soybean meal.

Inventors:
SUN ZHONGCHAO (CN)
JI CHUNYUAN (CN)
SHI ZEXU (CN)
LIU JIANGYING (CN)
LI SHICHAO (CN)
Application Number:
PCT/CN2018/124799
Publication Date:
July 02, 2020
Filing Date:
December 28, 2018
Export Citation:
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Assignee:
SHANGHAI YUANYAO BIOLOGICAL CO LTD (CN)
International Classes:
A23K10/10
Foreign References:
CN107343561A2017-11-14
CN103478417A2014-01-01
CN104012755A2014-09-03
CN108056272A2018-05-22
CN104186935A2014-12-10
CN104000006A2014-08-27
Attorney, Agent or Firm:
CO-HORIZON INTELLECTUAL PROPERTY INC. (CN)
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