Title:
METHOD FOR PREPARING HEMATOPOIETIC CELLS
Document Type and Number:
WIPO Patent Application WO/2017/038958
Kind Code:
A1
Abstract:
The invention pertains to a method for preparing hematopoietic cells of primates, characterized by including: a first step for culturing a cell group of pluripotent stem cells of a primate under conditions suited to inducing differentiation into hematopoietic cells, and obtaining a cell group including CD-34 negative cells; a second step for transplanting at least some of the cell group obtained in the first step into the fetus of an animal different from the primate; and a third step for obtaining hematopoietic cells of the primate from the body of the animal obtained by raising the offspring obtained by the birth of the fetus.
Inventors:
HANAZONO YUTAKA (JP)
ABE TOMOYUKI (JP)
NAGAO YOSHIKAZU (JP)
ABE TOMOYUKI (JP)
NAGAO YOSHIKAZU (JP)
Application Number:
PCT/JP2016/075743
Publication Date:
March 09, 2017
Filing Date:
August 26, 2016
Export Citation:
Assignee:
UNIV JICHI MEDICAL (JP)
UNIV UTSUNOMIYA (JP)
UNIV UTSUNOMIYA (JP)
International Classes:
C12N5/22; A01K67/027; C12N15/00; C12N15/09
Other References:
SASAKI, KYOKO ET AL.: "Primate Embryonic Stem Cells Differentiated In Vitro Engraft and Constitute Long-Term Hematopoiesis after Injection into Fetal Sheep", BLOOD, vol. 102, no. 11, 2003, pages 341a, XP002904155
NAGAO, YOSHIKAZU ET AL.: "Improved Efficacy and Safety of In Utero Cell Transplantation in Sheep Using an Ultrasound-Guided Method", CLONING AND STEM CELLS, vol. 11, no. 2, 2009, pages 281 - 285, XP055372637
NORIMASA IHARA ET AL.: "Shikyunai Zoketsu Kansaibo Ishokugo no Donor Lymph-kyu Yuchu de 100% Chimera to Naru Mechanism no Kento", ACTA OBSTETRICA ET GYNAECOLOGIA JAPONICA, vol. 61, no. 2, 2009, pages 664, ISSN: 0300 9165
TOMOYUKI ABE ET AL.: "Hitsuji Shikyunai Ishoku -kei ni Okeru Hito Zoketsu Saibo no Seichaku· Zofuku Gijutsu no Kaihatsu", JAPANESE ASSOCIATION FOR LABORATORY ANIMAL SCIENCE SOKAI KOEN YOSHISHU, vol. 61, 2014, pages 153
NAGAO, YOSHIKAZU ET AL.: "Improved Efficacy and Safety of In Utero Cell Transplantation in Sheep Using an Ultrasound-Guided Method", CLONING AND STEM CELLS, vol. 11, no. 2, 2009, pages 281 - 285, XP055372637
NORIMASA IHARA ET AL.: "Shikyunai Zoketsu Kansaibo Ishokugo no Donor Lymph-kyu Yuchu de 100% Chimera to Naru Mechanism no Kento", ACTA OBSTETRICA ET GYNAECOLOGIA JAPONICA, vol. 61, no. 2, 2009, pages 664, ISSN: 0300 9165
TOMOYUKI ABE ET AL.: "Hitsuji Shikyunai Ishoku -kei ni Okeru Hito Zoketsu Saibo no Seichaku· Zofuku Gijutsu no Kaihatsu", JAPANESE ASSOCIATION FOR LABORATORY ANIMAL SCIENCE SOKAI KOEN YOSHISHU, vol. 61, 2014, pages 153
Attorney, Agent or Firm:
ONO, Shinjiro et al. (JP)
Download PDF:
Previous Patent: PHOTOCHROMIC COATING COMPOSITION
Next Patent: KIT PREPARATION AND DOSE ADJUSTMENT METHOD
Next Patent: KIT PREPARATION AND DOSE ADJUSTMENT METHOD