Title:
METHOD FOR PREPARING HETERODIMERIC SNAKE VENOM PROTEIN
Document Type and Number:
WIPO Patent Application WO/2018/068347
Kind Code:
A1
Abstract:
Provided are recombinant plasmids containing the heterodimeric snake venom protein Agkisacutacin A chain gene and Agkisacutacin B chain gene, respectively, cell strains containing the recombinant plasmids, and a method for expressing the heterodimeric snake venom protein Agkisacutacin. The expression level of Agkisacutacin in the present method exceeds 10mg/L, and the purity level can reach more than 95% by means of two steps of purification.
Inventors:
DAI XIANGRONG (CN)
LI XIAOYI (CN)
QIAN FANG (CN)
LI XIAOYI (CN)
QIAN FANG (CN)
Application Number:
PCT/CN2016/104175
Publication Date:
April 19, 2018
Filing Date:
November 01, 2016
Export Citation:
Assignee:
ZHAOKE PHARMACEUTICAL HEFEI COMPANY LTD (CN)
International Classes:
C12N15/81; A61K38/17; A61P9/10; C07K1/18; C07K14/46; C12N1/19; C12N15/64
Foreign References:
| CN103263663A | 2013-08-28 | |||
| CN103263662A | 2013-08-28 | |||
| CN101838323A | 2010-09-22 |
Other References:
DATABASE Genbank 13 May 2002 (2002-05-13), ANONYMOUS, XP055592110, retrieved from NUCLEOTIDE Database accession no. AY091759.1
DATABASE Genbank 21 January 2002 (2002-01-21), ANONYMOUS, XP055592113, retrieved from NUCLEOTIDE Database accession no. AF463521.1
DATABASE Genbank 13 May 2002 (2002-05-13), ANONYMOUS, XP055592117, retrieved from PROTEIN Database accession no. AAM22787.1
DATABASE Genbank 21 January 2002 (2002-01-21), ANONYMOUS, XP055592120, retrieved from PROTEIN Database accession no. AAL66390.1
GUO, YUGANG ET AL.: "Balancing the Expression and Production of a Heterodimeric Protein: Recombinant Agkisacutacin as a Novel Antithrombotic Drug Candidate", SCIENTIFIC REPORTS, vol. 5, 6 July 2015 (2015-07-06), pages 11730, XP055475490, ISSN: 2045-2322, Retrieved from the Internet
HU , SIYI: "Expression and structural function of a smoked group of genetically engineered antibodies and snake venom C-type lectin-like proteins in Pichia pastoris", DOCTORAL DISSERTATION AND MASTER'S THESES FULL-TEXT DATABASES (DOCTORAL), 15 April 2007 (2007-04-15), pages 1 - 118, XP009515779, ISSN: 1671-6779
See also references of EP 3536791A4
DATABASE Genbank 21 January 2002 (2002-01-21), ANONYMOUS, XP055592113, retrieved from NUCLEOTIDE Database accession no. AF463521.1
DATABASE Genbank 13 May 2002 (2002-05-13), ANONYMOUS, XP055592117, retrieved from PROTEIN Database accession no. AAM22787.1
DATABASE Genbank 21 January 2002 (2002-01-21), ANONYMOUS, XP055592120, retrieved from PROTEIN Database accession no. AAL66390.1
GUO, YUGANG ET AL.: "Balancing the Expression and Production of a Heterodimeric Protein: Recombinant Agkisacutacin as a Novel Antithrombotic Drug Candidate", SCIENTIFIC REPORTS, vol. 5, 6 July 2015 (2015-07-06), pages 11730, XP055475490, ISSN: 2045-2322, Retrieved from the Internet
HU , SIYI: "Expression and structural function of a smoked group of genetically engineered antibodies and snake venom C-type lectin-like proteins in Pichia pastoris", DOCTORAL DISSERTATION AND MASTER'S THESES FULL-TEXT DATABASES (DOCTORAL), 15 April 2007 (2007-04-15), pages 1 - 118, XP009515779, ISSN: 1671-6779
See also references of EP 3536791A4
Attorney, Agent or Firm:
BEIJING TONGHUI INTELLECTUAL PROPERTY OFFICE (GENERAL PARTNERSHIP) (CN)
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