Title:
METHOD FOR PREPARING IMMOBILIZED ARGININE DEIMINASE AND PRODUCING [14/15N]-L-CITRULLINE
Document Type and Number:
WIPO Patent Application WO/2021/088603
Kind Code:
A1
Abstract:
Provided is an arginine deiminase-containing genetic engineering corynebacterium glutamicum having a preservation number of CGMCC No.19404, which expresses a cipA-arc fusion protein. The engineering bacteria and the cipA-arc fusion protein can both be used for converting [14/15N]-L-arginine for producing [14/15N]-L-citrulline.
Inventors:
HANG GANG (CN)
LI BIN (CN)
LI YUE (CN)
LI BIN (CN)
LI YUE (CN)
Application Number:
PCT/CN2020/120719
Publication Date:
May 14, 2021
Filing Date:
October 13, 2020
Export Citation:
Assignee:
SHANGHAI UNIV OF MEDICINE&HEALTH SCIENCES (CN)
International Classes:
C12N1/21; C07K19/00; C12N9/78; C12N15/77; C12P13/10; C12P21/02; C12R1/15
Domestic Patent References:
| WO2014138319A2 | 2014-09-12 |
Foreign References:
| CN101993867A | 2011-03-30 |
Other References:
MA YUE , SU LINGQIA , WU DAN , WU JING: "Optimization of Preparing L-citrulline by Recombinant Arginine Deiminase", BIOTECHNOLOGY BULLETIN, vol. 31, no. 8, 31 December 2015 (2015-12-31), pages 180 - 185, XP055811410, ISSN: 1002-5464, DOI: 10.13560/j.cnki.biotech.bull.1985.2015.08.026
DATABASE Protein 11 April 2001 (2001-04-11), ANONYMOUS: "crystalline inclusion protein type II [Photorhabdus luminescens subsp. luminescens]", XP055811387, retrieved from NCBI Database accession no. AAA27616
DATABASE Protein 16 March 2007 (2007-03-16), ANONYMOUS: "arginine deiminase [Lactococcus lactis subsp. lactis", XP055811394, retrieved from NCBI Database accession no. ABC88383
CAI XUE, JIANG HANGYU, ZHANG TAO, JIANG BO, MU WANMENG, MIAO MING: "Thermostability and specific-activity enhancement of an arginine deiminase from Enterococcus faecalis SK23.001 via semirational design for L-citrulline production.", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 66, no. 33, 31 December 2018 (2018-12-31), pages 8841 - 8850, XP055811400, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.8b02858
DATABASE Protein 11 April 2001 (2001-04-11), ANONYMOUS: "crystalline inclusion protein type II [Photorhabdus luminescens subsp. luminescens]", XP055811387, retrieved from NCBI Database accession no. AAA27616
DATABASE Protein 16 March 2007 (2007-03-16), ANONYMOUS: "arginine deiminase [Lactococcus lactis subsp. lactis", XP055811394, retrieved from NCBI Database accession no. ABC88383
CAI XUE, JIANG HANGYU, ZHANG TAO, JIANG BO, MU WANMENG, MIAO MING: "Thermostability and specific-activity enhancement of an arginine deiminase from Enterococcus faecalis SK23.001 via semirational design for L-citrulline production.", JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol. 66, no. 33, 31 December 2018 (2018-12-31), pages 8841 - 8850, XP055811400, ISSN: 0021-8561, DOI: 10.1021/acs.jafc.8b02858
Attorney, Agent or Firm:
D&S IP LAW FIRM (CN)
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