Title:
METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELLS USING A DEDIFFERENTIATION ENHANCER
Document Type and Number:
WIPO Patent Application WO/2012/141471
Kind Code:
A3
Abstract:
The present invention relates to a method for preparing induced pluripotent stem cells (iPS cells), comprising: (a) culturing human-derived somatic cells, into which genes encoding differentiation-inducing factors are introduced, in a medium containing one or more dedifferentiation enhancers selected from a group consisting of a protein kinase C inhibitor, a histone deacetylase inhibitor, and a bone morphogenetic protein (BMP) pathway blocker; and (b) separating embryonic stem cell-like colonies from the culture obtained in step (a). The method of the present invention may enable the preparation of induced pluripotent stem cells with high efficiency, as well as the preparation of induced pluripotent stem cells under xenopathogen-free and feeder cell-free conditions.
Inventors:
HWANG DONG-YOUN (KR)
LEE KANG-IN (KR)
LEE KANG-IN (KR)
Application Number:
PCT/KR2012/002696
Publication Date:
December 06, 2012
Filing Date:
April 10, 2012
Export Citation:
Assignee:
COLLEGE MEDICINE POCHON CHA UNIV IND ACAD COOP FOUND (KR)
HWANG DONG-YOUN (KR)
LEE KANG-IN (KR)
HWANG DONG-YOUN (KR)
LEE KANG-IN (KR)
International Classes:
C12N5/0735; C12N5/02
Foreign References:
| US20100173414A1 | 2010-07-08 |
Other References:
ZHU ET AL.: "Reprogramming of human primary somatic cells by Oct4 and chemical compounds", CELL STEM CELL, vol. 7, 3 December 2010 (2010-12-03), pages 651 - 655, XP055075780, DOI: doi:10.1016/j.stem.2010.11.015
HUANGFU ET AL.: "Induction of pluripotent stem stem cells by defined factors is greatly improved by small-molecule compounds", NATURE BIOTECHNOLOGY, vol. 26, no. 7, 22 June 2008 (2008-06-22), pages 795 - 797, XP002502536, DOI: doi:10.1038/NBT1418
DUTTA ET AL.: "Self-renewal versus lineage commitment of embryonic stem cells: protein kinase C signaling shifts the balance", STEM CELLS, vol. 29, 3 February 2011 (2011-02-03), pages 618 - 628
ZHOU ET AL.: "High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors", STEM CELLS, vol. 28, 23 August 2010 (2010-08-23), pages 1741 - 1750
HAO ET AL.: "Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells", PLOS ONE, vol. 3, no. IS.8, 6 August 2008 (2008-08-06), pages 1 - 8
HUANGFU ET AL.: "Induction of pluripotent stem stem cells by defined factors is greatly improved by small-molecule compounds", NATURE BIOTECHNOLOGY, vol. 26, no. 7, 22 June 2008 (2008-06-22), pages 795 - 797, XP002502536, DOI: doi:10.1038/NBT1418
DUTTA ET AL.: "Self-renewal versus lineage commitment of embryonic stem cells: protein kinase C signaling shifts the balance", STEM CELLS, vol. 29, 3 February 2011 (2011-02-03), pages 618 - 628
ZHOU ET AL.: "High-efficiency induction of neural conversion in human ESCs and human induced pluripotent stem cells with a single chemical inhibitor of transforming growth factor beta superfamily receptors", STEM CELLS, vol. 28, 23 August 2010 (2010-08-23), pages 1741 - 1750
HAO ET AL.: "Dorsomorphin, a selective small molecule inhibitor of BMP signaling, promotes cardiomyogenesis in embryonic stem cells", PLOS ONE, vol. 3, no. IS.8, 6 August 2008 (2008-08-06), pages 1 - 8
Attorney, Agent or Firm:
OH, Kook-Jin (1708-6 Seocho-dong, Seocho-gu, Seoul 137-884, KR)
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Claims: