Title:
METHOD FOR PREPARING LIBRARY FOR HIGHLY PARALLEL SEQUENCING BY USING MOLECULAR BARCODING, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/204572
Kind Code:
A1
Abstract:
Provided is a method for preparing a library for highly parallel sequencing, comprising the steps of: providing two or more double stranded nucleic acid molecules; attaching adaptors to both ends of each of the nucleic acid molecules; providing a primer pair for amplifying each of the nucleic acid molecules, wherein each of the primers constituting the primer pair comprises i) a 3'-end region comprising a nucleotide sequence complementary to the adaptor, ii) a 5'-end region comprising a universal primer sequence for a highly parallel sequencing, and iii) an index sequence region positioned between the 3'- and 5'-end regions, and an index sequence of one of the primer pair is a unique molecular sequence unique to each of the nucleic acid molecules and the index sequence of the other primer is a sample indication sequence for indicating a sample from which the nucleic acid molecule is derived; and performing an amplification reaction by using the primer pair, so as to produce an amplification product of each of the nucleic acid molecules comprising the unique molecular sequence and the sample indication sequence.
Inventors:
KIM HYO KI (KR)
HAN HYO JUN (KR)
SEO SUNG HYUN (KR)
JANG HOON (KR)
HAN HYO JUN (KR)
SEO SUNG HYUN (KR)
JANG HOON (KR)
Application Number:
PCT/KR2017/005455
Publication Date:
November 30, 2017
Filing Date:
May 25, 2017
Export Citation:
Assignee:
CELEMICS INC (KR)
International Classes:
C12Q1/68; G16B30/10
Domestic Patent References:
| WO2014071361A1 | 2014-05-08 | |||
| WO2015097030A1 | 2015-07-02 |
Foreign References:
| US20130231253A1 | 2013-09-05 |
Other References:
HEAD ET AL.: "Library Construction for Next-generation Sequencing: Overviews and Challenges", BIOTECHNIQUES, vol. 56, no. 2, February 2014 (2014-02-01), pages 61 - 64,66,68-70,72-74,76-77, XP002740381
FU ET AL.: "Molecular Indexing Enables Quantitative Targeted RNA Sequencing and Reveals Poor Efficiencies in Standard Library Preparations", PNAS, vol. 111, no. 5, 4 February 2014 (2014-02-04), pages 1891 - 1896, XP055164122
FU ET AL.: "Molecular Indexing Enables Quantitative Targeted RNA Sequencing and Reveals Poor Efficiencies in Standard Library Preparations", PNAS, vol. 111, no. 5, 4 February 2014 (2014-02-04), pages 1891 - 1896, XP055164122
Attorney, Agent or Firm:
KWAK, Hyun Kyu et al. (KR)
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