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Title:
METHOD FOR PREPARING MESO-2,3-BUTANEDIOL
Document Type and Number:
WIPO Patent Application WO/2012/124890
Kind Code:
A3
Abstract:
The present invention relates to a method for preparing meso-2,3-butanediol. More particularly, the present invention relates to colon bacillus for overexpressing meso-2,3-butaediol, which is cotransformed to an expression vector including at least one nucleotide SEQ ID selected from the group consisting of the following nucleotide SEQ IDs: (a) nucleotide-coding acetoin reductase having an amino acid sequence described in SEQ ID 5 of a sequence listing; (b) nucleotide-coding acetolactate decarboxylase; and (c) nucleotide-coding alcohol dehydrogenase. The cotransformed colon bacillus of the present invention can produce 2,3-butanediol, which could not be obtained from wild species. Therefore, it is possible to biosynthesize a large amount of meso-2,3-butanediol through the biosynthesis path expression of meso-2,3-butanediol from glucose, and through metabolic flux change by means of the insertion of the genes of other species.

Inventors:
LEE JIN WON (KR)
LEE SOO JIN (KR)
KIM BO-RIM (KR)
CHOI WOO JOO (KR)
Application Number:
PCT/KR2012/000436
Publication Date:
December 27, 2012
Filing Date:
January 18, 2012
Export Citation:
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Assignee:
UNIV SOGANG IND UNIV COOP FOUN (KR)
LEE JIN WON (KR)
LEE SOO JIN (KR)
KIM BO-RIM (KR)
CHOI WOO JOO (KR)
International Classes:
C12N1/21; C12N15/53; C12N15/60; C12N15/70; C12P7/16
Other References:
YAJUN YAN ET AL.: "Enantioselective synthesis of pure(R,R)-2,3-butane-diol in Escherichia coli with stereospecific secondary alcohol dehydro-genases", ORGANIC AND BIOMOLECULAR CHEMISTRY, vol. 7, no. 19, 2009, pages 3914 - 3917
SADAHARU UI ET AL.: "Molecular generation of an Escherichia coli strain producing only the meso-isomer of 2,3-butanediol", JOURNAL OF FERMENTATION AND BIOENGINEERING., vol. 84, no. 3, 1997, pages 185 - 189
KRISTINA BLOMQVIST ET AL.: "Characterization of the genes of the 2,3-butanediol operons from Klebsiella terrigena and Enterobacter aerogenes", JOURNAL OF BACTERIOLOGY, vol. 175, no. 5, March 1993 (1993-03-01), pages 1392 - 1404
DAVID R. NIELSEN ET AL.: "Metabolic engineering of acetoin and meso-2,3-butanediol biosynthesis in E. coli", BIOTECHNOLOGY JOURNAL., vol. 5, no. 3, March 2010 (2010-03-01), pages 274 - 284
DATABASE GENBANK 2 August 2009 (2009-08-02), "Klebsiella oxytoca strain M5al alpha- acetolactate decarboxylase(budA) and alpha-actolactate synthase (budB) genes, complete cds; and acetoin (diacetyl) reductase (budC) gene, partial cds", accession no. Q253371
DATABASE GENBANK 6 January 2011 (2011-01-06), "Acetolactate decarboxylase [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]", accession no. P 002919835
DATABASE GENBANK 6 January 2011 (2011-01-06), "Acetoin reductase [Klebsiella pneumoniae subsp. pneumoniae NTUH-K2044]", accession no. P 002919837
Attorney, Agent or Firm:
YANG, Boo-Hyun (1659-2 Inhundong, Gwanak-gu, Seoul 151-832, KR)
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Claims: