Title:
METHOD FOR PREPARING NANOPARTICLE WITH HYDROPHILIC LIGAND AND ANTIBODY CONJUGATED TO SURFACE THEREOF, NANOPARTICLE PREPARED THEREBY, COMPOSITE INCLUDING SAME NANOPARTICLE, AND DIAGNOSTIC KIT INCLUDING SAME NANOPARTICLE
Document Type and Number:
WIPO Patent Application WO/2022/177041
Kind Code:
A1
Abstract:
The present invention relates to a method for preparing nanoparticles having a hydrophilic ligand and an antibody conjugated to the surface thereof, the method comprising the steps of: preparing nanoparticles having a hydrophilic ligand conjugated to the surface thereof; diluting the nanoparticles in a buffer to form a nanoparticle mixed buffer; mixing an antibody in the nanoparticle mixed buffer and stirring same to form a nanoparticle-antibody conjugate mixed buffer; and adding a blocking solution to the nanoparticle-antibody conjugate mixed buffer and stirring same to form a blocked nanoparticle-antibody conjugate mixed buffer, whereby an antibody can be directly conjugated without needing an additional chemical linker, with the consequent improvement of efficiency and reproducibility in production process.
Inventors:
JEON JIN WOO (KR)
HA NA RUM (KR)
KIM TAE YONG (KR)
SONG AH YOUNG (KR)
HA NA RUM (KR)
KIM TAE YONG (KR)
SONG AH YOUNG (KR)
Application Number:
PCT/KR2021/002143
Publication Date:
August 25, 2022
Filing Date:
February 19, 2021
Export Citation:
Assignee:
GREEN CROSS MEDICAL SCIENCE (KR)
International Classes:
G01N33/531; G01N33/543
Foreign References:
| KR20170097520A | 2017-08-28 | |||
| KR20180072014A | 2018-06-29 | |||
| KR20070088393A | 2007-08-29 | |||
| KR20210100848A | 2021-08-18 |
Other References:
KUMAR S, AARON J, SOKOLOV K: "Directional conjugation of antibodies to nanoparticles for synthesis of multiplexed optical contrast agents with both delivery and targeting moieties.", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 3, no. 2, 1 January 2008 (2008-01-01), GB , pages 314 - 320, XP008102097, ISSN: 1750-2799, DOI: 10.1038/NPROT.2008.1
ZHANG BINGBO, WANG XIAOHUI, LIU FENGJUN, CHENG YINGSHENG, SHI DONGLU: "Effective Reduction of Nonspecific Binding by Surface Engineering of Quantum Dots with Bovine Serum Albumin for Cell-Targeted Imaging", LANGMUIR, AMERICAN CHEMICAL SOCIETY, US, vol. 28, no. 48, 4 December 2012 (2012-12-04), US , pages 16605 - 16613, XP055962383, ISSN: 0743-7463, DOI: 10.1021/la302758g
American Scientific Publishers ; 1 January 2016 (2016-01-01), SINGH GURPAL, KUMAR MANOJ, SONI UDIT, ARORA VIKAS, BANSAL VIVEK, GUPTA DIKSHI, BHAT MADHUSUDAN, DINDA AMIT K, SAPRA SAMEER, SINGH : "Cancer Cell Targeting Using Folic Acid/Anti-HER2 Antibody Conjugated Fluorescent CdSe/CdS/ZnS-Mercaptopropionic Acid and CdTe-Mercaptosuccinic Acid Quantum Dots", XP009539249
ZHANG BINGBO, WANG XIAOHUI, LIU FENGJUN, CHENG YINGSHENG, SHI DONGLU: "Effective Reduction of Nonspecific Binding by Surface Engineering of Quantum Dots with Bovine Serum Albumin for Cell-Targeted Imaging", LANGMUIR, AMERICAN CHEMICAL SOCIETY, US, vol. 28, no. 48, 4 December 2012 (2012-12-04), US , pages 16605 - 16613, XP055962383, ISSN: 0743-7463, DOI: 10.1021/la302758g
American Scientific Publishers ; 1 January 2016 (2016-01-01), SINGH GURPAL, KUMAR MANOJ, SONI UDIT, ARORA VIKAS, BANSAL VIVEK, GUPTA DIKSHI, BHAT MADHUSUDAN, DINDA AMIT K, SAPRA SAMEER, SINGH : "Cancer Cell Targeting Using Folic Acid/Anti-HER2 Antibody Conjugated Fluorescent CdSe/CdS/ZnS-Mercaptopropionic Acid and CdTe-Mercaptosuccinic Acid Quantum Dots", XP009539249
Attorney, Agent or Firm:
JUNG, Young Hoon (KR)
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