Title:
METHOD FOR PREPARING PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2015/033558
Kind Code:
A1
Abstract:
The objective of the present invention is to provide a low-cost, simple method for preparing cells having pluripotency and extremely low tumorigenic risk. Human mesenchymal stem cells from bone marrow (hMSC-BM), human adipose tissue-derived mesenchymal stem cells (hAT-MSC) (also known as "human adipose-derived stem cells (hADSC)")and other such mammal mesenchymal stem cells are cultured in suspension with 7 types of human adherent mature cell (human hepatocyte cells [hHEP cells], human umbilical vein endothelial cells [HUVEC cells], human dermal microlymphatic endothelial cells [HMVEC cells], human epidermal keratinocyte cells [NHEK cells], human bronchial epithelial cells [NHBE cells], human melanocyte cells [NHEM cells], and human smooth muscle cells [UASMC cells]) and 3 types of human adherent precursor cell (human dermal fibroblast cells [NHDF], human skeletal muscle myoblast cells [HSMM cells], and human osteoblast cells [NHOst cells]) in order to form a mass of cells (spheroid), thereby enabling the preparation of cells having pluripotency and extremely low tumorigenic risk.
Inventors:
OKAIRI RISA (JP)
NISHIMURA MASUHIRO (JP)
WADA TAMAKI (JP)
NISHIMURA MASUHIRO (JP)
WADA TAMAKI (JP)
Application Number:
PCT/JP2014/004524
Publication Date:
March 12, 2015
Filing Date:
September 03, 2014
Export Citation:
Assignee:
OTSUKA PHARMA CO LTD (JP)
International Classes:
A61K35/12; C12N5/0775; A61P43/00; C12N5/02; C12N15/09
Domestic Patent References:
| WO2012133942A1 | 2012-10-04 | |||
| WO2011007900A1 | 2011-01-20 | |||
| WO2006085612A1 | 2006-08-17 | |||
| WO2008150001A1 | 2008-12-11 | |||
| WO2012063870A1 | 2012-05-18 | |||
| WO1999064565A2 | 1999-12-16 |
Foreign References:
| JP5185443B2 | 2013-04-17 | |||
| JP2002291469A | 2002-10-08 | |||
| JP2004121165A | 2004-04-22 | |||
| JP2003505006A | 2003-02-12 |
Other References:
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TAKAHASHI, K. ET AL., CELL, vol. 131, 2007, pages 861 - 872
NAKAGAWA, M. ET AL., NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
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BOHELER K.R ET AL., CIRC. RES., vol. 91, 2002, pages 189 - 201
KLUG M. G ET AL., J. CLIN. INVEST., vol. 98, 1996, pages 216 - 224
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VITTET D ET AL., PROC. NATL. ACAD. SCI. USA, vol. 94, 1997, pages 6273 - 6278
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IKEDA H ET AL., PROC. NATL. ACAD. SCI. USA, vol. 102, 2005, pages 11331 - 11336
OSAKADA F ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 215 - 224
OSAKADA F ET AL., NAT. PROTOC., vol. 4, 2009, pages 811 - 824
HIRAMI Y ET AL., NEUROSCI. LETT, vol. 458, 2009, pages 126 - 131
OSAKADA F ET AL., J CELL SCI, vol. 122, 2009, pages 3169 - 3179
SENJU S; HARUTA M; MATSUNAGA Y ET AL., STEM CELLS, vol. 27, 2009, pages 1021 - 1031
LIAN ET AL., PNAS, vol. 109, no. 27, 2012
GROPP ET AL., PLOS ONE, vol. 7, no. 9, 2012
TAKAHASHI, K. ET AL., CELL, vol. 131, 2007, pages 861 - 872
NAKAGAWA, M. ET AL., NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
WERING, M. ET AL., CELL STEM CELL, vol. 2, 2008, pages 10 - 12
KURODA, Y. ET AL., PROC NATL ACAD SCI U S A., vol. 107, 2010, pages 8639 - 8643
WADA ET AL., PLOS ONE, vol. 4, no. 8, 2009, pages E6722
BOHELER K.R ET AL., CIRC. RES., vol. 91, 2002, pages 189 - 201
KLUG M. G ET AL., J. CLIN. INVEST., vol. 98, 1996, pages 216 - 224
MULLER M ET AL., FASEB. J., vol. 14, 2000, pages 2540 - 2548
VITTET D ET AL., PROC. NATL. ACAD. SCI. USA, vol. 94, 1997, pages 6273 - 6278
BLOCH W ET AL., J. CELL BIOL., vol. 139, 1997, pages 265 - 278
YAMASHITA J ET AL., NATURE, vol. 408, 2000, pages 92 - 96
FERAUD 0 ET AL., LAB. INVEST, vol. 81, 2001, pages 1669 - 1681
IKEDA H ET AL., PROC. NATL. ACAD. SCI. USA, vol. 102, 2005, pages 11331 - 11336
OSAKADA F ET AL., NAT. BIOTECHNOL., vol. 26, 2008, pages 215 - 224
OSAKADA F ET AL., NAT. PROTOC., vol. 4, 2009, pages 811 - 824
HIRAMI Y ET AL., NEUROSCI. LETT, vol. 458, 2009, pages 126 - 131
OSAKADA F ET AL., J CELL SCI, vol. 122, 2009, pages 3169 - 3179
SENJU S; HARUTA M; MATSUNAGA Y ET AL., STEM CELLS, vol. 27, 2009, pages 1021 - 1031
LIAN ET AL., PNAS, vol. 109, no. 27, 2012
GROPP ET AL., PLOS ONE, vol. 7, no. 9, 2012
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
Masaki Hirota (JP)
Masaki Hirota (JP)
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