Title:
METHOD FOR PREPARING RECOMBINANT ESCHERICHIA COLI TO PRODUCE SUCCINIC ACID THROUGH FERMENTATION
Document Type and Number:
WIPO Patent Application WO/2012/119546
Kind Code:
A3
Abstract:
A method for preparing recombinant Escherichia coli to produce succinic acid through fermentation. The method mainly involves increasing the synthesis rate of succinic acid by overexpressing NAD+ biosynthesis-related genes in Escherichia coli, and adding a certain concentration of nicotinic acid to the anaerobic culture medium as the precursor material of NAD+ biosynthesis, thus increasing the strain growth rate in anaerobic conditions and the synthesis rate of succinic acid.
Inventors:
JIANG MIN (CN)
MA JIANGFENG (CN)
CHEN KEQUAN (CN)
GUO TING (CN)
WEI PING (CN)
OUYANG PINGKAI (CN)
MA JIANGFENG (CN)
CHEN KEQUAN (CN)
GUO TING (CN)
WEI PING (CN)
OUYANG PINGKAI (CN)
Application Number:
PCT/CN2012/072012
Publication Date:
November 01, 2012
Filing Date:
March 06, 2012
Export Citation:
Assignee:
UNIV NANJING (CN)
JIANG MIN (CN)
MA JIANGFENG (CN)
CHEN KEQUAN (CN)
GUO TING (CN)
WEI PING (CN)
OUYANG PINGKAI (CN)
JIANG MIN (CN)
MA JIANGFENG (CN)
CHEN KEQUAN (CN)
GUO TING (CN)
WEI PING (CN)
OUYANG PINGKAI (CN)
International Classes:
C12N1/21; C12N15/52; C12N15/54; C12N15/63; C12P7/46; C12R1/19
Foreign References:
| CN102174458A | 2011-09-07 | |||
| CN102154339A | 2011-08-17 | |||
| CN101044245A | 2007-09-26 | |||
| CN1202930A | 1998-12-23 |
Other References:
LIU, RONGMING ET AL.: "Effect of overexpression of nicotinic acid phosphoribosyl transferase on succinic acid production in Escherichia coli NZN111", CHINESE JOURNAL OF BIOTECHNOLOGY, 25 October 2011 (2011-10-25), pages 1438 - 1447
VEMURI, G.N. ET AL.: "Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 4, 28 April 2002 (2002-04-28), pages 1715 - 1727
JIANG, MIN ET AL.: "The Progress of Recombinant Escherichia coli for Production of Succinic Acid", MICROBIOLOGY, vol. 36, no. 1, 20 January 2009 (2009-01-20), pages 120 - 124
QIN, YI ET AL.: "Manipulation of NADH metabolism in industrial strains", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 25, no. 2, 25 February 2009 (2009-02-25), pages 161 - 169
VEMURI, G.N. ET AL.: "Effects of Growth Mode and Pyruvate Carboxylase on Succinic Acid Production by Metabolically Engineered Strains of Escherichia coli", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 68, no. 4, 28 April 2002 (2002-04-28), pages 1715 - 1727
JIANG, MIN ET AL.: "The Progress of Recombinant Escherichia coli for Production of Succinic Acid", MICROBIOLOGY, vol. 36, no. 1, 20 January 2009 (2009-01-20), pages 120 - 124
QIN, YI ET AL.: "Manipulation of NADH metabolism in industrial strains", CHINESE JOURNAL OF BIOTECHNOLOGY, vol. 25, no. 2, 25 February 2009 (2009-02-25), pages 161 - 169
Attorney, Agent or Firm:
JIANGSU CO-FAR LAW FIRM (No.6 Shitoucheng Rd. Gulo, Nanjing Jiangsu 3, CN)
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